Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
Cui, Lunbiao; Ge, Yiyue; Qi, Yuhua; Shi, Zhiyang; Jiao, Yongjun; Qi, Xian; Zhai, Xiangjun; Wang, Hua.
Braz. J. Microbiol.;
41(2)2010.
Artigo
em Inglês
| VETINDEX
| ID: vti-444546
Resumo
The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.
Biblioteca responsável:
BR68.1
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