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Biosurfactant production by Rhodococcus erythropolis and its application to oil removal

Jardim Pacheco, Graziela; Mara Prioli Ciapina, Elisa; de Barros Gomes, Edelvio; Pereira Junior, Nei.
Braz. J. Microbiol.; 41(3)2010.
Artigo em Inglês | VETINDEX | ID: vti-444565

Resumo

The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3,MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-associated production was detected. Reduction in the surface tension, which indicates the presence of biosurfactant, reached a value of 38 mN/m at the end of 35 hours. Use of the produced biosurfactant for washing crude oil-contaminated soil showed that 2 and 4 times the critical micellar concentration (CMC) were able to remove 97 and 99% of the oil, respectively, after 1 month of impregnation.
Biblioteca responsável: BR68.1