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Growth inhibition and morphological alterations of Trichophyton rubrum induced by essential oil from Cymbopogon winterianus Jowitt ex Bor
de Oliveira Pereira, Fillipe; Alves Wanderley, Paulo; Antônio Cavalcanti Viana, Fernando; Baltazar de Lima, Rita; Barbosa de Sousa, Frederico; de Oliveira Lima, Edeltrudes.
Afiliação
  • de Oliveira Pereira, Fillipe; Universidade Federal da Paraíba Centro de Ciências da Saúde Departamento de Ciências Farmacêuticas.
  • Alves Wanderley, Paulo; Universidade Federal da Paraíba Centro de Formação de Tecnólogos Departamento de Agricultura.
  • Antônio Cavalcanti Viana, Fernando; Universidade Federal da Paraíba Centro de Ciências da Saúde Laboratório de Tecnologia Farmacêutica Horto de Plantas Medicinais.
  • Baltazar de Lima, Rita; Universidade Federal da Paraíba Centro de Ciências Exatas e da Natureza Departamento de Sistemática e Ecologia.
  • Barbosa de Sousa, Frederico; Universidade Federal da Paraíba Centro de Ciências da Saúde Laboratório de Microscopia e Imagem Biológica.
  • de Oliveira Lima, Edeltrudes; Universidade Federal da Paraíba Centro de Ciências da Saúde Departamento de Ciências Farmacêuticas.
Article em En | VETINDEX | ID: vti-444653
Biblioteca responsável: BR68.1
ABSTRACT
Trichophyton rubrum is one of the most common fungi causer of dermatophytosis, mycosis that affect humans and animals around the world. Researches aiming new products with antifungal activity become necessary to overcome difficulties on treatment of these infections. Accordingly, this study aimed to investigate the antifungal activity of essential oil from Cymbopogon winterianus against the dermatophyte T. rubrum. The antifungal screening was performed by solid medium diffusion method with 16 T. rubrum strains, minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined using the microdilution method. The effects on mycelial dry weight and morphology were also observed. Screening showed essential oil in natura inhibited all the tested strains, with inhibition zones between 24-28 mm diameter. MIC50 and MIC90 values of the essential oil were 312 µg/mL for nearly all the essayed strains (93.75 %) while the MFC50 and MFC90 values were about eight times higher than MIC for all tested strains. All tested essential oil concentrations managed to inhibit strongly the mycelium development. Main morphological changes on the fungal strains observed under light microscopy, which were provided by the essential oil include loss of conidiation, alterations concerning form and pigmentation of hyphae. In the oil presence, colonies showed folds, cream color and slightly darker than the control, pigment production was absent on the reverse and with evident folds. It is concluded that C. winterianus essential oil showed activity against T. rubrum. Therefore, it could be known as potential antifungal compound especially for protection against dermatophytosis.
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2011 Tipo de documento: Article
Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2011 Tipo de documento: Article