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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Miyata, Marcelo; Carlos Barreto Santos, Adolfo; Helena Mendes, Natália; Atsuko Cunha, Eunice; Augusto Fiúza de Melo, Fernando; Queico Fujimura Leite, Clarice.
Braz. J. Microbiol.; 42(2)2011.
Artigo em Inglês | VETINDEX | ID: vti-444719

Resumo

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
Biblioteca responsável: BR68.1