Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
de Almeida Vaucher, Rodrigo; Dezen, Diogenes; Braga Simonetti, Amauri; Rosado Spilki, Fernando; Michel Roehe, Paulo.
Braz. J. Microbiol.;
42(4)2011.
Artigo
em Inglês
| VETINDEX
| ID: vti-444804
Resumo
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Biblioteca responsável:
BR68.1
Texto completo