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A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Qiu, Shanlian; Chen, Jichen; Lin, Si; Lin, Xinjian.
Braz. J. Microbiol.; 43(2)2012.
Artigo em Inglês | VETINDEX | ID: vti-444904

Resumo

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
Biblioteca responsável: BR68.1