Dietary levels of Mannanoligosaccharide (MOS) for nile-tilapia (Oreochromis niloticus): Intestinal Morphology / Níveis de mananoligossacarídeos (MOS) na dieta de Tilápias-do-Nilo (Oreochromis niloticus): Morfologia intestinal

ABSTRACT

Intensification of aquaculture production systems exposes fish to numerous stressors, which may negatively affect their growth and limit profitability of aquaculture systems. In addition, fish farmers are now obliged to conform to Best Management Practices (BPMs) regulations. This current setup favors the use of dietary prebiotics for management of farmed fish as environmentally friendly practice. Mannanoligosaccharides (MOS) are complex carbohydrates derived from yeast (Saccharomyces cerevisiae) cell walls. This study was set out to evaluate the effects of increasing levels of dietary MOS on intestinal morphology of Nile-tilapia. Fish (12.62 ± 0.38 ) were randomly distributed into 16 cages (0.25m3 polyvinyl chloride; 20 fish per cage), inside four 5m3 net-cage at Salto Caxias Hydroeletric water reservoir (Boa Vista da Aparecida, PR) and fed diets containing increasing levels of MOS (0.0; 0.2; 0.4 and 0.8%) during 30 days (n=4). The proximal intestine fragment of two specimens from each replicate was taken for histological observations. The histological sections (5 m) were stained with haematoxylin and eosin (H & E) and documented photographically with a digital camera (DCM 130E digital camera for microscope 1.3 pixels, CMOS chip Software Scopephoto) connected to a light microscope (EDUTEC 502 AC). The images were analyzed by using (BEL Eurisko) software for intestinal vil

RESUMO

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