Abdominal hernia repair with bovine pericardium seeded with mesenchymal stem cells in Wistar rats / Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar
Bellini Vidor, Silvana; Maria Volpato Marques, Janete; Fidelis da Luz Moura, Lanucha; Gomes, Cristiano; Helena Paz, Ana; Martins Gomes, Herbert; Meurer, Luíse; Rodrigues de Barros, Rodrigo; Magano Guimarães, Karina; Obino Cirne-Lima, Elizabeth; Antônio Contesini, Emerson.
Acta sci. vet. (Online);
41: 01-10, 2013.
Artigo
em Português
| VETINDEX
| ID: vti-480171
Resumo
Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the influence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented efficiency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytesVários tipos de implantes, naturais ou sintéticos, vêm sendo testados no reparo cirúrgico de hérnias. As membranas biológicas, ou arcabouços dérmicos descelularizados, apresentam reduzida formação de aderência entre o implante e as vísceras, diminuição da formação de fístulas, infecções e recorrências. Também apresentam resistência suficiente para suportar a pressão abdominal, evitando deiscências e eviscerações. [...]
Biblioteca responsável:
BR68.1
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