Characterization of Francisella species isolated from the cooling water of an air conditioning system

Gu, Quan; Li, Xunde; Qu, Pinghua; Hou, Shuiping; Li, Juntao; Atwill, Edward R; Chen, Shouyi

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Characterization of Francisella species isolated from the cooling water of an air conditioning system

Gu, Quan; Li, Xunde; Qu, Pinghua; Hou, Shuiping; Li, Juntao; Atwill, Edward R; Chen, Shouyi.

Braz. J. Microbiol.; 46(3): 921-927, July-Sept. 2015. tab, ilus

Artigo em Inglês | VETINDEX | ID: vti-481686

Resumo

Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive. In addition, these bacteria grow on cysteine-supplemented media at 20 °C to 40 °C with an optimal growth temperature of 30 °C. Analysis of 16S rRNA gene sequences revealed that these strains belong to the genus Francisella. Biochemical tests and phylogenetic and BLAST analyses of 16S rRNA, rpoB and sdhA genes indicated that one strain was very similar to Francisella philomiragia and that the other strains were identical or highly similar to the Francisella guangzhouensis sp. nov. strain 08HL01032 we previously described. Biochemical and molecular characteristics of these strains demonstrated that multiple Francisella species exist in air conditioning systems..(AU)

Assuntos

Ar Condicionado Proteínas de Bactérias/genética RNA Polimerases Dirigidas por DNA/genética Flavoproteínas/genética Francisella Microbiologia da Água Sequência de Bases China DNA Bacteriano/genética DNA Ribossômico/genética Francisella/classificação Francisella/genética Francisella/isolamento & purificação Dados de Sequência Molecular Tipagem Molecular Filogenia RNA Ribossômico 16S/genética Análise de Sequência de DNA

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Characterization of Francisella species isolated from the cooling water of an air conditioning system

Resumo

Assuntos