Padronizacão da técnica de imunoistoquímica para o diagnóstico etiológico de rotina da diarréia bovina a vírus / Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine
Andrade, G. I; Serra, C. V; Barbosa-Stancioli, E. F; Serakides, R; Lobato, Z. I. P.
Arq. bras. med. vet. zootec;
57(5): 710-714, Oct. 2005. ilus, tab
Artigo
em Português
| VETINDEX
| ID: vti-6511
Resumo
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.(AU)
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