Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin / Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation
Silva, D. G; Fagliari, J. J; Garcia, T. B.
Arq. bras. med. vet. zootec;
60(3): 766-768, jun. 2008. tab
Artigo
em Português
| VETINDEX
| ID: vti-6775
Resumo
The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of Salmonella Dublin (P<0,05), whereas RV broth incubated at 42ºC had a lower efficiency in the microbiological isolation.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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