Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium
Gonçalves, Rodrigo José de Sousa; Cavalcante, Agnes Yasmin Pitombeira; Gouveia, Bruna Bortoloni; Lins, Thae Lanne Barbosa Gama; Barberino, Ricássio de Sousa; Menezes, Vanúzia Gonçalves; Barros, Vanessa Raquel Pinto de; Santos, Luciana da Paz dos; Santos, Jamile Maiara da Silva; Figueiredo, José Ricardo de; Matos, Maria Helena Tavares de.
Acta Vet. bras.;
11(1): 50-56, mar. 2017. ilus, tab, graf
Artigo
em Inglês
| VETINDEX
| ID: vti-687016
Resumo
This study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium - MEM without supplementation or supplemented MEM, i.e. MEM+) and stored at 35ºC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented alfa-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM+ for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM+ for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM+) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35ºC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.(AU)
Biblioteca responsável:
BR68.1
Localização: BR68.1
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