Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Oliveira, GH; Berchieri Jr, A; Montassier, HJ.
R. bras. Ci. avíc.;
8(1)2006.
Artigo
em Inglês
| VETINDEX
| ID: vti-717741
Resumo
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Biblioteca responsável:
BR68.1
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