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Follicular histomorphometry and evaluation of ovarian apoptosis in queens of different age groups

Carvalho, Isadora Resende de; Denadai, Daniela Scantamburlo; Trevisan, Juliane Teramachi; Mendes, Heloisa Maria Falcão; Perri, Sílvia Helena Venturoli; Monteiro, Cristina Maria Rodrigues; Ferreira, Joana Zafalon; Feliciano, Marcus Antonio Rossi; Pierucci, Julia Cestari; Vicente, Wilter Ricardo Russiano; Koivisto, Marion Burkhardt de.
Acta sci. vet. (Online); 44: 01-08, 2016. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-722694


Background: In humans and bitchs the age is another factor that may affect the size of ovarian structures, verifying alterations in the quality of the pool and size of follicular structures, which can compromise the use of these structures for in vitro maturation. There are no reports correlating the morphometric characteristics of the follicles and ovarian apoptosis at different ages in cats. The aim of this study was to evaluate the histomorphometric parameters of follicular growth and the relationship with the occurrence of apoptosis in ovarian tissue of young, adult and senile queens. Materials, Methods & Results: Eighteen domestic queens, multiparous, of different breeds and age groups were used in this study and divided into three groups according to their ages: five months to one year - young; (7.8 ± 1.0 months); one to six years - adults (2.8 ± 0.5 years); and more than six years - senile (8.0 ± 0.9 years). Vaginal cytology was performed in order to characterize the estrous phase associated with plasma concentrations of progesterone. The morphology and percentage of the vaginal epithelium cells were evaluated and queens were classified into estrous and non-estrus and plasma concentrations of progesterone were determined. Ovarian samples were collected after ovariohysterectomy to routine histological processin and all follicles were counted and categorized into two [...](AU)
Biblioteca responsável: BR68.1
Localização: BR68.1