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Primeira evidência da infecção pelo vírus da diarreia viral bovina em javalis / First evidence of bovine viral diarrhea virus infection in wild boars
Weber, Matheus Nunes; Pino, Eloisa Helena Moreira; Souza, Carine Kunzler; Mósena, Ana Cristina Sbaraini; Sato, José Paulo Hiroji; Barcellos, David Emílio Santos Neves de; Canal, Cláudio Wageck.
Afiliação
  • Weber, Matheus Nunes; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre. Brasil
  • Pino, Eloisa Helena Moreira; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre. Brasil
  • Souza, Carine Kunzler; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre. Brasil
  • Mósena, Ana Cristina Sbaraini; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre. Brasil
  • Sato, José Paulo Hiroji; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Setor de Suínos. Porto Alegre. Brasil
  • Barcellos, David Emílio Santos Neves de; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Setor de Suínos. Porto Alegre. Brasil
  • Canal, Cláudio Wageck; Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre. Brasil
Acta sci. vet. (Online) ; 44: 01-05, 2016. graf
Article em Pt | VETINDEX | ID: vti-722709
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Background:

The farming of wild boars has growing due to the interest of the human consumption of this exotic meat. Such a development may pose an increased risk of disease transmission between boars and domestic animals. The wild boar population has increased in South America in the last years due the absence of predator causing economic losses due to direct damage to crops and risk of disease transmission. The genus Pestivirus within the family Flaviviridae are composed by four recognized species by the International Committee on the Taxonomy of Viruses (ICTV) classical swine fever virus (CSFV), border disease virus (BDV), bovine viral diarrhea virus type 1 (BVDV-1) and 2 (BVDV-2). Other putative species denoted as atypical pesitiviruses have been reported as ‘HoBi-like virus, giraffe pestivirus, Bungowannah pestivirus, Pronghorn antelope virus, atypical porcine pestivirus (APPV), Norwegian rat pestivirus (NrPV) and Rhinolophus affinis bat pestivirus (RaPestV-1). CSFV is commonly detected in wild boars, but despite positive serology, bovine viral diarrhea virus (BVDV) was never detected in this animal species. Thereby, the present communication describes the first detection of BVDV in the lungs of captive boars using RT-PCR and DNA sequencing.Materials, Methods &

Results:

Forty lung samples from farmed wild boars were collected after slaughter in a commercial abattoir. The organs were crushed separately, centrifuged, and the supernatant was stored for further analysis. The total RNA was isolated using a phenol-based protocol and RT-PCR protocol that amplified 118 bp of 5 untranslated region (5UTR) was carried out. One out 40 samples resulted positive. The positive sample had partial fragments of 5UTR and N terminal autoprotease (Npro) sequenced and analyzed. The strain LV Java/2012 presented 99% of identity in 5UTR and 98% in Npro region with a BVDV-2 previously reported in bovines in Southern Brazil.[...](AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: Pt Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2016 Tipo de documento: Article
Texto completo: 1 Base de dados: VETINDEX Idioma: Pt Revista: Acta sci. vet. (Impr.) / Acta sci. vet. (Online) Ano de publicação: 2016 Tipo de documento: Article