Diagnosis of early pregnancy in the throughbred mare by the toad test - Resulte of its utilization during fifteen breeding seasons / Bufo-reação no diagnóstico precoce da prenhez na égua puro sanque inglês - Resultados do seu emprego durante quinze estações de monta

ABSTRACT

The results with the utilization of the male toad test for the diagnosis of pregnancy in the Thoroughbred mare, during fifteen breeding season, were described. The experiments were performed on male toads (Bufo morinus L. and Bufo paracnemis Lutz) varying in weight from 90 to 320 g. Galli Mainini"s technique with some modification was used. Blood serum samples were drawn from 313 mares thirty-six to 120 days following the date of the last breeding. A single mare-serum injection of 12 to 15 ml was given subcutaneously into the latero-caudal lymph sac of the toad. Two toads were used for each test. Positive reactions, caracterized by spermatozoa found in toad"s urine, had resulted mainly within three to six hours. As reasonable but not strict aseptic procedures followed the collection of the blood-serum samples, some investigations were undertaken, (a) with putrid positive serum and (b) with positive serum sample preserved by the addition of sodium mertiolate in the proposition of 110.000 or sodium sulfatiazol in the concentration of 0,2 per cent. In (a) all the toads gave positive results and a great number of them died about 24 hours after the injection. In (b), after 13 months of conservation, all the samples gave also positive results, and were not toxic for the toads. Of the 782 test that were performed, 593 positive and 150 negative reactions were in agreement. Among the r

RESUMO

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