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Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus
Shakibaie, Mojtaba; Ameri, Atefeh; Ghazanfarian, Roya; Adeli-Sardou, Mahboubeh; Amirpour-Rostami, Sahar; Torkzadeh-Mahani, Masoud; Imani, Mehdi; Forootanfar, Hamid.
Afiliação
  • Shakibaie, Mojtaba; Kerman University of Medical Sciences. Herbal and Traditional Medicines Research Center. Kerman. Iran
  • Ameri, Atefeh; Kerman University of Medical Sciences. Institute of Neuropharmacology. Pharmaceutics Research Center. Kerman. Iran
  • Ghazanfarian, Roya; Kerman University of Medical Sciences. Faculty of Pharmacy. The Student Research Committee. Kerman. Iran
  • Adeli-Sardou, Mahboubeh; Kerman University of Medical Sciences. Herbal and Traditional Medicines Research Center. Kerman. Iran
  • Amirpour-Rostami, Sahar; Kerman University of Medical Sciences. Institute of Neuropharmacology. Pharmaceutics Research Center. Kerman. Iran
  • Torkzadeh-Mahani, Masoud; University of Advanced Technology. Research institute for Science and High Technology and Environmental Sciences. Department of Biotechnology. Kerman. Iran
  • Imani, Mehdi; Urmia University. Faculty of Veterinary Medicine. Department of Basic Sciences. Urmia. Iran
  • Forootanfar, Hamid; Kerman University of Medical Sciences. Faculty of Pharmacy. Department of Pharmaceutical Biotechnology. Kerman. Iran
Braz. J. Microbiol. ; 49(4): 865-871, Oct.-Dec. 2018. ilus, tab, graf
Article em En | VETINDEX | ID: vti-738177
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21 g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (540 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25-1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most considerable factors within the tested levels (p < 0.05). The optimum medium composition for the kojic acid production by the C5-10 mutant was found to be glucose, 98.4 g/L; yeast extract, 1.0 g/L; and KH2PO4, 10.3 mM which was theoretically able to produce 120.2 g/L of kojic acid based on the obtained response surface model for medium optimization. Using these medium compositions an experimental maximum Kojic acid production (109.0 ± 10 g/L) was acquired which verified the efficiency of the applied method.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2018 Tipo de documento: Article / Project document

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2018 Tipo de documento: Article / Project document