Your browser doesn't support javascript.
loading
Stereological and morphological analysis of the effects of aging on spermatogenesis in rat testis
Miranda, Eduardo Pereira de; Lorenzini, Fernando; Neves, Bruno Vinicius Duarte; Melchioretto, Eduardo Felippe; Hota, Thiago; Fraga, Rogerio De.
Afiliação
  • Miranda, Eduardo Pereira de; s.af
  • Lorenzini, Fernando; Universidade Federal do Paraná. School of Medicine. Department of Urology. Curitiba. Brasil
  • Neves, Bruno Vinicius Duarte; Universidade Federal do Paraná. School of Medicine. Department of Urology. Curitiba. Brasil
  • Melchioretto, Eduardo Felippe; Universidade Federal do Paraná. School of Medicine. Department of Urology. Curitiba. Brasil
  • Hota, Thiago; Universidade Federal do Paraná. School of Medicine. Department of Urology. Curitiba. Brasil
  • Fraga, Rogerio De; Universidade Federal do Paraná. School of Medicine. Department of Urology. Curitiba. Brasil
Acta cir. bras. ; 33(10): 904-913, Oct. 2018. ilus, tab, graf
Article em En | VETINDEX | ID: vti-738753
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To quantify, through stereological and morphometric analysis, spermatogenesis in rats undergoing the natural aging process.

Methods:

Seventy-two male Wistar rats were divided into 6 equal groups according to age at the time of killing 3, 6, 9, 12, 18, and 24 months. All the rats were subjected orchiectomy and collection of testicular parenchymal fragments for histological and morphometric analysis. The numerical density of spermatids was calculated using a stereological study, and morphometric analysis was conducted to measure the height of the germinal epithelium and the area of the seminiferous tubules.

Results:

We found that the 18 and 24 months groups showed a significant reduction in the number of round spermatids. However, the height of the germinal epithelium was not significantly different between the groups. The area of seminiferous tubules was also significantly reduced in the elderly rats compared to that in the young ones.

Conclusion:

Aging of rats showed a significant reduction in the number of round spermatids and the area of the seminiferous tubules, more pronounced in the rats at 18 and 24 months of life.(AU)
Assuntos
Palavras-chave