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Molecular Investigations of the Effect of Thermal Manipulation During Embryogenesis on the Enzymatic Activity of Creatine Kinase and Lactate Dehydrogenase in Broiler Muscle
Dalab, A. S; Ali, A. M; Althnaian, T. A; Alkhodair, K. M; Al-Ramadan, S. Y.
Afiliação
  • Dalab, A. S; An-Najah National University. College of Agriculture and Veterinary Medicine. Department of Veterinary Medicine. Nablus. Saudi Arabia
  • Ali, A. M; King Faisal University. College of Veterinary Medicine. Department of Anatomy. Nablus. Saudi Arabia
  • Althnaian, T. A; King Faisal University. College of Veterinary Medicine. Department of Anatomy. Nablus. Saudi Arabia
  • Alkhodair, K. M; King Faisal University. College of Veterinary Medicine. Department of Anatomy. Nablus. Saudi Arabia
  • Al-Ramadan, S. Y; King Faisal University. College of Veterinary Medicine. Department of Anatomy. Nablus. Saudi Arabia
R. bras. Ci. avíc. ; 24(1): eRBCA-2020-1369, 2022. tab, graf
Article em En | VETINDEX | ID: vti-765873
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Incubating temperature and timing or duration is critical to determine the optimum protocol of thermal manipulation (TM), which underlines muscle growth improvement. Therefore, the aim of the present study is to determine the optimum period of embryonic TM that may result in the improvement of pectoral and thigh muscle myogenesis. This is done by investigating the level of mRNA expression of creatine kinase (CK) and lactate dehydrogenase (LDH). An additional goal is measuring the blood levels of CK and LDH as a biomarker of muscle injury due to the experimental thermal challenge on post-hatch day 35. The study was conducted on 1,440 fertile eggs (Ross broilers) that were divided randomly and equally into a control group and four treatment groups (TM1, TM2, TM3, and TM4). The treatment groups were daily subjected to TM at 39 ºC for 18h with 65% relative humidity (RH) during embryonic days (EDs) 7-11, 11-15, 15-18, and 7-18, respectively. Among the thermally manipulated groups that were investigated, TM1 (ED 7-11) resulted in significant improvement of mRNA expression and enzymatic concentration of CK and LDH in muscle during embryogenesis, as compared to the control. Six hours of TC showed the highest significant CK and LDH expression and concentration levels in the control as compared to TM groups. Thus, the results of this study indicate that TM during ED 7-11 improves pectoral and thigh muscles response to heat stress without adversely affecting their performance. This finding could be used by commercial breeders to enhance local broiler production.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: R. bras. Ci. avíc. Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: R. bras. Ci. avíc. Ano de publicação: 2022 Tipo de documento: Article