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Assessment of the effectiveness of the PPD-mallein produced in Brazil for diagnosing glanders in mules
Silva, Karla Patrícia Chaves da; Takaki, Galba Maria de Campos; Silva, Leonildo Bento Galiza da; Saukas, Tomoe Noda; Santos, André Souza; Mota, Rinaldo Aparecido.
Afiliação
  • Silva, Karla Patrícia Chaves da; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
  • Takaki, Galba Maria de Campos; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
  • Silva, Leonildo Bento Galiza da; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
  • Saukas, Tomoe Noda; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
  • Santos, André Souza; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
  • Mota, Rinaldo Aparecido; Universidade Federal de Alagoas. Departamento de Medicina Veterinária. Maceió. Brasil
Braz. J. Microbiol. ; 44(1): 179-188, 2013. ilus
Article em En | VETINDEX | ID: vti-7974
Biblioteca responsável: BR1.1
Localização: BR68.1
ABSTRACT
To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichlo-roa-cetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no inflammatory reaction at the site of inoculation of PPD-mallein. This immunogen produced in Brazil and still being tested was effective for identifying the infection in true positive animals and excluding the truly negative ones, being a new possibility for diagnosis and control of glanders.(AU)
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2013 Tipo de documento: Article