Superoxid dismutase activity in portal vein endothelium after partial liver resection
Sayão Filho, Rogério Heggendorn; Perini, Marcos Vinicius; Cruz, José Arnaldo; Requena, Juliana; Barbeiro, Hermes Vieira; Molan, Nilza Trindade; Lopasso, Fabio Pinatel; D'Albuquerque, Luiz Augusto Carneiro; Cecconello, Ivan.
Acta cir. bras.;
28(9): 646-651, Sept. 2013. graf, tab
Artigo
em Inglês
| VETINDEX
| ID: vti-9040
Resumo
PURPOSE: To investigate superoxide dismutase (SOD) activity in the portal vein endothelium and malondialdehyde acid (MDA) production in liver tissue of rats submitted to 70% hepatectomy. METHODS:Twelve rats were distributed in two groups (hepatectomy and sham). Animals were sacrificed on post operative day 1 and portal vein, liver tissue and blood samples were collected. Portal vein SOD production was measured using lucigenin-amplified chemiluminescence assays. MDA measurement was used as an index of oxidative stress through the formation of TBARS (Thiobarbituric Acid Reactive Species). RESULTS: There was no difference in post operative bilirrubin, AST, ALT levels between groups. DHL level was higher in the hepatectomy group (p=0.01). MDA production in the remnant liver tissue and endothelial portal vein SOD activity were also significantly (p<0.05) elevated in the hepatectomy group when compared to control group. There was no correlation between MDA and SOD activity. SOD activity, on the other hand, showed a positive correlation with LDH level (p=0.038) and MDA levels showed a positive correlation with AST and ALT levels (p<0.001). CONCLUSION: There is an increased production of malondialdehyde acid in liver tissue after partial hepatectomy and increased activity of superoxide dismutase in portal vein endothelium as well.(AU)
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