Portal de Pesquisa da BVS Veterinária

Tese em Português | VETTESES | ID: vtt-207294

Resumo

O objetivo deste trabalho foi avaliar a composicao quimica, desempenho, parametros bioquimicos e hematologicos e o potencial antimicrobiano frente a Aeromonas ssp. dos oleos essenciais de capim limao Cymbopogon flexuosus (OECF) e manjericao Ocimum basilicum (OEOB) e os seus efeitos como aditivos na dieta de juvenis de tilapias do Nilo. O estudo foi desenvolvido atraves de ensaios in vitro e in vivo. No ensaio in vitro foram utilizados 24 isolados de Aeromonas spp. Os peixes foram distribuidos em 20 tanques em sistema com aeracao constante. No experimento in vivo foram avaliadas cinco dietas com niveis crescentes dos oleos essenciais (0; 0,25; 0,50; 1,0 e 2,0 mL/kg) e quatro repeticoes. Ao final foi realizada biometria, coleta de sangue, retirada de figado e intestino, para analise de parametros desempenho, bioquimico e hematologico. Os peixes que restaram em cada tratamento foram desafiados por Aeromonas spp. O - citral (50,13%) e ß-citral (40,31%) foram os componentes majoritarios do OECF, e linalol (53,35%) do OEOB. Os testes in vitro mostraram que o OECF apresentou elevada atividade inibitoria sobre Aeromonas spp., enquanto que o OEOB para a mesma bacteria apresentou atividade inibitoria moderada. A inclusao do OECF na dieta reduziu conversao alimentar aparente, glicose, triglicerides e lisozima do plasma e glicogenio hepatico e aumentou sobrevivencia pre e pos desafio, ganho de peso, taxa de crescimento, indice hepatossomatico, rendimento de carcaca; proteinas totais, aminoacidos totais, colesterol e ALT do plasma, ALT hepatica, amilase intestinal e parametros hematologicos. Com a inclusao do OEOB na dieta reduziu conversao alimentar aparente, rendimento de carcaca, glicose, triglicerideos e lisozima do plasma e glicogenio e ALT hepatico e aumentou ganho de peso, taxa de crescimento, proteinas totais, aminoacidos totais, colesterol e ALT do plasma e hemoglobina. As concentracoes mais altas do OEOB aumentaram as enzimas digestivas. Conclui-se que ambos oleos essenciais sao promissores como aditivos fitogenicos e podem contribuir na melhora de desempenho, parametros bioquimicos e hematologicos, porem na taxa de sobrevivencia pos-desafio com Aeromonas spp. somente OECF.

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The objective of this work was to evaluate the chemical composition, performance, biochemical and hematological parameters and antimicrobial potential against Aeromonas ssp. for the essential oils of lemongrass Cymbopogon flexuosus (EOCF) and basil Ocimum basilicum (EOOB) and their effects as additives in the diet of juveniles of Nile tilapia. The study was developed through in vitro and in vivo assays. In the in vitro assay, 24 isolates of Aeromonas spp. The fish were distributed in 20 tanks in a system with constant aeration. In the in vivo experiment, five diets with increasing levels of essential oils (0, 0.25, 0.50, 1.0 and 2.0 mL / kg) and four replicates were evaluated. Biometrics, blood collection, liver removal and intestine were performed for performance, biochemical and hematological parameters. The fish that remained in each treatment were challenged by Aeromonas spp. -citral (50.13%) and -citral (40.31%) were the major components of EOCF, and linalool (53.35%) of EOOB. In vitro tests showed that EOCF showed high inhibitory activity on Aeromonas spp., whereas EOOB for the same bacterium showed moderate inhibitory activity. The inclusion of EOCF in the diet reduced apparent feed conversion, glucose, triglycerides and plasma lysozyme and hepatic glycogen and increased pre and post challenge survival, weight gain, growth rate, hepatosomatic index, carcass yield, total proteins, total amino acids, plasma ALT and cholesterol, ALT hepatic, intestinal amylase and haematological parameters. With the inclusion of EOOB in the diet reduced apparent feed conversion, carcass yield, glucose, triglycerides and plasma lysozyme and glycogen and ALT hepatic and increased weight gain, growth rate, total proteins, total amino acids, cholesterol and ALT of plasma and hemoglobin. Higher concentrations of EOOB increased digestive enzymes. It is concluded that both essential oils are promising as phytogenic additives and may contribute to improved performance, biochemical and hematological parameters, but the post-challenge survival rate with Aeromonas spp. only EOCF.

Biblioteca responsável: BR68.1