Portal de Pesquisa da BVS Veterinária

Tese em Português | VETTESES | ID: vtt-213404

Resumo

Obesity is a transgenerational epigenetic metabolic disturbance. Animal models of obesity like zebrafish (Danio rerio) are useful to understand the signaling pathways involved on this condition. In this study, we analyzed, on zebrafish, the effect of maternity and paternity obesity on reproduction and offspring (F1). Animals of both genders were used, divided into two groups; the control group (C) which received a balanced diet, and the obesity group induced by overfeeding (DIO). The feeding protocol was contucted for eight weeks and the animals were reproduced weekly. To verify the impact of obesity on the parents, we made histologic assessment through morphometry of adipose tissue (AT) visceral and subcutaneous, and we measured the hepatosomatic (GSI) and gonadosomatic (HSI) indexs. The impact of overfeeding was evaluated on reproduction, spawning, fertility and fertilization rate. On the offspring were analyzed the mortality rate, hatching, lordosis, and tail deformity. Glucose and triglycerides plasma levels were measure in zebrafish adult and offspring. As a result, we identified that the induction of obesity caused hypertrophy on AT and an increase of HSI, in both sexes and an increase of GSI on the females. The obesity not caused infertility of the zebrafish. However, overfeeding caused high mortality rate of offspring, morphologic deformities and high plasmatic levels of glucose and triglycerides, with 100% of mortality at 60 dpf. We concluded that the induction of obesity through overfeeding didnt have any effect on the reproductive capacity of zebrafish, however, it caused negative effects on its offspring with the high occurrence of deformities and mortality.

---

Obesity is a transgenerational epigenetic metabolic disturbance. Animal models of obesity like zebrafish (Danio rerio) are useful to understand the signaling pathways involved on this condition. In this study, we analyzed, on zebrafish, the effect of maternity and paternity obesity on reproduction and offspring (F1). Animals of both genders were used, divided into two groups; the control group (C) which received a balanced diet, and the obesity group induced by overfeeding (DIO). The feeding protocol was contucted for eight weeks and the animals were reproduced weekly. To verify the impact of obesity on the parents, we made histologic assessment through morphometry of adipose tissue (AT) visceral and subcutaneous, and we measured the hepatosomatic (GSI) and gonadosomatic (HSI) indexs. The impact of overfeeding was evaluated on reproduction, spawning, fertility and fertilization rate. On the offspring were analyzed the mortality rate, hatching, lordosis, and tail deformity. Glucose and triglycerides plasma levels were measure in zebrafish adult and offspring. As a result, we identified that the induction of obesity caused hypertrophy on AT and an increase of HSI, in both sexes and an increase of GSI on the females. The obesity not caused infertility of the zebrafish. However, overfeeding caused high mortality rate of offspring, morphologic deformities and high plasmatic levels of glucose and triglycerides, with 100% of mortality at 60 dpf. We concluded that the induction of obesity through overfeeding didnt have any effect on the reproductive capacity of zebrafish, however, it caused negative effects on its offspring with the high occurrence of deformities and mortality.

Biblioteca responsável: BR68.1