Portal de Pesquisa da BVS Veterinária

Tese em Português | VETTESES | ID: vtt-216925

Resumo

Trinta ovelhas, mesticas (Santa Ines X Dorper), adultas e higidas foram selecionadas para avaliar o efeito da suplementacao intramuscular com as vitaminas D e E no perfil bioquimico, metabolismo energetico, metabolismo oxidativo, sensibilidade insulinica e imunidade no periparto. Apos confirmacao da gestacao essas femeas foram distribuidas em tres grupos de dez animais e no 108o dia de gestacao receberam veiculo oleoso (grupo controle-GC); ou 70.000 UI/kg de P.V. de vitamina D3 (colecalciferol) (grupo tratado-GD); ou 60UI/kg de P.V. de vitamina E (-tocoferol) (grupo tratado-GE). As amostras de sangue foram coletadas previamente a aplicacao da vitamina (-45), quatro (-30); e duas semanas (-15) antes do parto; ate duas horas do parto (0), uma (7); duas (15); e quatro semanas apos o parto (30). Foram analisadas as concentracoes plasmaticas de glicose, beta hidroxibutirato (BHB), acidos graxos nao esterificados (AGNEs) e as concentracoes sericas de colesterol, triglicerides, ureia, creatinina, calcio total, calcio ionico, proteina total, acido urico, aspartato amino transferase (AST), gamaglutamil transferase (GGT), e ainda a creatina quinase (CK). Foram tambem analisadas as concentracoes de insulina e cortisol. Do metabolismo oxidativo foram determinadas as atividades da superoxido dismutase (SOD) e glutationa peroxidase (GSH-Px), a habilidade de reducao ferrica plasmatica (HRFP), substancias reativas ao acido tiobarbiturico (TBARS), bem como o status antioxidante total (TAS). Para avaliar aspectos relacionados a imunidade de monocitos e neutrofilos, por metodo in vitro, em 24 ovelhas (seis de cada tratamento). As amostras de sangue foram coletadas nos momentos 30; 0; e 30 e avaliadas sem estimulo (burst basal) e com estimulo por DCFH-DA, SaPI e PMA. A imunofenotipagem com anticorpos monoclonais CD8, CD4, CD14, CD16, CD45R, WC1, CD206, foi realizada aos dias -30, -15,0,7 e 30. Foram determinadas as concentracoes das vitaminas D e E, nos momentos pre-determinados das coletas e no dia do teste de tolerancia a glicose (TTG), realizado para avaliar a sensibilidade insulinica, e foi calculado RQUICKI e RQUICKI BHB. Todas as variaveis estudadas 14 apresentaram efeito de tempo. Houve interacao tempo*tratamento em TBARS (P=0,0217) e tendencia para vitamina E (0,0811), SOD (0,0886) e GSH-Px (P=0,0643). Houve efeito de tratamento no colesterol (P=0,0088) e vitamina D (P<0,0001) e tendencia para TAS (P=0,0830). No TTG, com resultados de AUC de BHB e AGNE apresentaram efeito de numero de fetos gestados (P=0,0006 e 0,0005, respectivamente) e o RQUICKIBHB mostrou-se melhor indicador de sensibilidade insulinica do que o RQUICKI em ovelhas. A suplementacao com vitamina D influenciou os teores plasmaticos de alfa tocoferol e a resposta imune das ovelhas. A suplementacao com vitamina E reduziu a peroxidacao lipidica no parto e relacionouse positivamente com a melhora oxidativa dos monocitos sem estimulo e estimulados por PMA. Altas doses de vitamina D e E aos 45 dias pre-parto aparentemente sao beneficos, porem, mais estudos sobre o tema sao necessarios.

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Thirty healthy adult ewes were selected to evaluate the effect of supplementation with intramuscular vitamins D and E in peripartum period on the biochemical profile, energetic metabolism, oxidative metabolism, insulin sensitivity and immunity. After pregnancy confirmation these females were distributed into three groups of ten animals treated with oily vehicle (control-to-control group) on the 108th day of pregnancy, (CG); or 70,000 IU / kg of body weight of vitamin D3 (cholecalciferol) (treated group-GD); or 60 IU / kg of body weight of vitamin E (-tocopherol) (treated group-GE). Blood samples were collected before the application of the vitamins and vehicle (-45), four (-30); and two weeks (-15) before lambing; until 2 hours of lambing (0); one (7), two (15) and four weeks postpartum (30). Plasma concentrations of glucose, beta hydroxybutyrate (BHB), non-esterified fatty acids (NEFA) and serum concentrations of cholesterol, triglycerides, urea, creatinine, total calcium, ionic calcium, total protein, uric acid, aspartate amino transferase (AST), gammaglutamyl transferase (GGT), and creatine kinase (CK) were measured. The concentrations of insulin and cortisol, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), plasma ferric reduction ability (FRAP), thiobarbituric acid reactive substances (TBARS), as well as total antioxidant status (TAS) were determinate. Monocytes and neutrophils were assessed by in vitro method to identify aspects related to immunity in 24 ewes (six from each treatment) at -30, 0 and 30. Blood samples were evaluated without stimulus (basal burst), with DCFH-DA, SaPI and PMA stimulus. Immunophenotyping with monoclonal antibodies CD8, CD4, CD14, CD16, CD45R, WC1, CD206 were performed at -30, -15, 0, 7 and 30. The concentrations of vitamins D and E were determinate at the predetermined collection times and on the day of the Intravenous Glucose Tolerance Test (IVGTT), realized to evaluate the insulin sensitivity, as well as RQUICKI and RQUICKI BHB. All variables studied showed time effects. There were interaction time * treatment in TBARS (P = 0.0217) and tendency for vitamin E (0.0811), SOD (0.0886) and GSH-Px (P = 0.0643). 16 Treatment effect on cholesterol (P = 0.0088) and vitamin D (P <0.0001) and tendency for TAS (P = 0.0830) were observed. About of IVGTT, AUC results of BHB and AGNE showed number of fetuses effect (P = 0.0006 and 0.0005 respectively). RQUICKIBHB was a better indicator of insulin sensitivity than RQUICKI in ewes. Vitamin D supplementation influenced the plasma levels of alpha tocopherol and the immune response of ewes. Vitamin E supplementation reduced lipid peroxidation at parturition and was positively related to the oxidative improvement of the monocytes without stimulation and stimulated by PMA. High doses of vitamins D or E administered 45 days before parturition on healthy ewes can be beneficial; however more studies are needed about this topic.

Biblioteca responsável: BR68.1