Characterization of bacterial growth by means of flow microfluorometry.

By means of flow microfluorometry, the protein and nucleic acid contents of individual bacterial cells may be measured at the rate of several thousand cells per second. Accumulation of such information over a few minutes yields the composition distribution of the microbial population. These distributions have been determined at different times during batch growth of Bactillus subtilis, and the results indicate that the variance of cell composition decreases as the population passes through the exponential into the stationary phase. The relative abundance of endospores and vegetative cells as well as the protein distributions of these subpopulations may be readily determined from flow microfluorometry data. Experimental access to such details of microbial population dynamics should foster improved understanding of cell growth, spore germination, and spore formation kinetics.

Pion radiotherapy: studies with nuclear emulsions.

Nuclear emulsions were used to provide information on (1) the pion star distribution for a therapy beam; (2) star production as a function of pion energy and residual range in nuclear emulsion; (3) the distribution of nuclear framgent ranges in emulsion; and (4) the neutron energy spectrum and fluence produced by negative pion capture in tissue, during treatment of a patient. This last item is important for determining the whole-body dose delivered to a patient undergoing pion radiotherapy.

A subfemtotesla multichannel atomic magnetometer.

The magnetic field is one of the most fundamental and ubiquitous physical observables, carrying information about all electromagnetic phenomena. For the past 30 years, superconducting quantum interference devices (SQUIDs) operating at 4 K have been unchallenged as ultrahigh-sensitivity magnetic field detectors, with a sensitivity reaching down to 1 fT Hz(-1/2) (1 fT = 10(-15) T). They have enabled, for example, mapping of the magnetic fields produced by the brain, and localization of the underlying electrical activity (magnetoencephalography). Atomic magnetometers, based on detection of Larmor spin precession of optically pumped atoms, have approached similar levels of sensitivity using large measurement volumes, but have much lower sensitivity in the more compact designs required for magnetic imaging applications. Higher sensitivity and spatial resolution combined with non-cryogenic operation of atomic magnetometers would enable new applications, including the possibility of mapping non-invasively the cortical modules in the brain. Here we describe a new spin-exchange relaxation-free (SERF) atomic magnetometer, and demonstrate magnetic field sensitivity of 0.54 fT Hz(-1/2) with a measurement volume of only 0.3 cm3. Theoretical analysis shows that fundamental sensitivity limits of this device are below 0.01 fT Hz(-1/2). We also demonstrate simple multichannel operation of the magnetometer, and localization of magnetic field sources with a resolution of 2 mm.

High-sensitivity atomic magnetometer unaffected by spin-exchange relaxation.

Alkali-metal magnetometers compete with SQUID detectors as the most sensitive magnetic field sensors. Their sensitivity is limited by relaxation due to spin-exchange collisions. We demonstrate a K magnetometer in which spin-exchange relaxation is completely eliminated by operating at high K density and low magnetic field. Direct measurements of the signal-to-noise ratio give a magnetometer sensitivity of 10 fT Hz(-1/2), limited by magnetic noise produced by Johnson currents in the magnetic shields. We extend a previous theoretical analysis of spin exchange in low magnetic fields to arbitrary spin polarizations and estimate the shot-noise limit of the magnetometer to be 2x10(-18) T Hz(-1/2).