Effectiveness of antimicrobial photodynamic therapy with a single treatment of RLP068/Cl in an experimental model of Staphylococcus aureus wound infection.

BACKGROUND: Chronic leg ulceration is a common health problem. It is well known that a clinically relevant bacterial load in chronic cutaneous wounds interferes significantly with the normal process of healing. Staphylococcus aureus is the most important representative of the staphylococcal group which causes clinically relevant infections within immunocompetent patients. OBJECTIVES: To investigate the efficacy of a single treatment of antimicrobial photodynamic therapy (APDT) with RLP068/Cl in a mouse model of a surgical wound infection induced with a methicillin-resistant strain of S. aureus (MRSA). METHODS: Wounds, established through the panniculus carnosus of BALB/c and CD1 mice, were inoculated with 5 x 10(7) c.f.u. of MRSA. Mice were randomized into four groups respectively receiving no treatment, APDT with placebo, APDT with a new phthalocyanine derivative (RLP068/Cl) and intraperitoneal teicoplanin. RESULTS: On day 2 from infection, a strong reduction of bacterial counts (≈ 3 logs) was observed in mice treated with RLP068/Cl in comparison with infected untreated mice. On day 9 from infection, a comparable and significant (≈ 2 logs) reduction of bacterial counts was found in mice treated with RLP068/Cl or with teicoplanin. At this time, histological examinations revealed that wounds treated with RLP068/Cl showed a complete re-epithelialization with a continuous epithelial lining. CONCLUSIONS: The results of the in vivo study demonstrated that APDT with RLP068/Cl may be useful in the management of chronic infected wounds, accelerating the repair process through a significant bacterial inhibition.

Anabolic steroid use by adolescents: prevalence, motives, and knowledge of risks.

Athletes and nonathletes use anabolic-androgenic steroids (AAS) to improve their strength and enhance their appearance. Few studies have been undertaken in the 1990s to assess the use of AAS in adolescents, following changes in legislation. This study was conducted to determine the prevalence of AAS use by high school students in Denver, Colorado, an area with high sports participation. A confidential questionnaire was completed by 6,930 students (response rate 96.6%) at 10 Denver high schools. The prevalence of AAS use was 2.7% (4.0% for boys and 1.3% for girls). Prevalence was slightly higher in sport participants than nonparticipants. The mean age of starting AAS was 14 years (range 8-17 years). This study is the first documented detailed assessment of high school students' knowledge of the risks of AAS. It shows knowledge deficits regarding potential side effects. Users of AAS were less likely than nonusers to acknowledge the risks of AAS. Only 18% of students claim to have been informed about AAS by physicians. The results suggest that health care providers should provide more information to adolescents about AAS. If educational efforts are found effective, teaching efforts regarding AAS should start in junior high school or sooner and continue through high school.

Femoral anteversion and arthritis of the knee.

Anteversion and arthritic changes were recorded in 110 well-preserved femora to identify a correlation between knee osteoarthritis and anteversion. Arthritis was recorded as no change (grade 0), osteophytes (grade 1), cysts or erosions (grade 2), and burnishing or eburnation (grade 3). Anteversion was identified by measure of the angle between the axis of the head-neck and the axis of the posterior condyles. There was no relation between anteversion and proximal femoral arthritis but increased arthritis of the distal femur with decreased anteversion (p < 0.04) was identified, establishing a correlation between femoral anteversion and arthritis of the knee.