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1.
Biochemistry ; 52(48): 8663-76, 2013 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-24215428

RESUMO

Cyanobacterial phycobiliproteins have evolved to capture light energy over most of the visible spectrum due to their bilin chromophores, which are linear tetrapyrroles that have been covalently attached by enzymes called bilin lyases. We report here the crystal structure of a bilin lyase of the CpcS family from Thermosynechococcus elongatus (TeCpcS-III). TeCpcS-III is a 10-stranded ß barrel with two alpha helices and belongs to the lipocalin structural family. TeCpcS-III catalyzes both cognate as well as noncognate bilin attachment to a variety of phycobiliprotein subunits. TeCpcS-III ligates phycocyanobilin, phycoerythrobilin, and phytochromobilin to the alpha and beta subunits of allophycocyanin and to the beta subunit of phycocyanin at the Cys82-equivalent position in all cases. The active form of TeCpcS-III is a dimer, which is consistent with the structure observed in the crystal. With the use of the UnaG protein and its association with bilirubin as a guide, a model for the association between the native substrate, phycocyanobilin, and TeCpcS was produced.


Assuntos
Proteínas de Bactérias/química , Cianobactérias/enzimologia , Liases/química , Ficobiliproteínas/química , Sequência de Aminoácidos , Cristalografia por Raios X , Modelos Moleculares , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Análise Espectral
2.
Microorganisms ; 11(10)2023 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-37894251

RESUMO

Oxygenic photosynthesis requires metal-rich cofactors and electron-transfer components that can produce reactive oxygen species (ROS) that are highly toxic to cyanobacterial cells. Biliverdin reductase (BvdR) reduces biliverdin IXα to bilirubin, which is a potent scavenger of radicals and ROS. The enzyme is widespread in mammals but is also found in many cyanobacteria. We show that a previously described bvdR mutant of Synechocystis sp. PCC 6803 contained a secondary deletion mutation in the cpcB gene. The bvdR gene from Synechococcus sp. PCC 7002 was expressed in Escherichia coli, and recombinant BvdR was purified and shown to reduce biliverdin to bilirubin. The bvdR gene was successfully inactivated in Synechococcus sp. PCC 7002, a strain that is naturally much more tolerant of high light and ROS than Synechocystis sp. PCC 6803. The bvdR mutant strain, BR2, had lower total phycobiliprotein and chlorophyll levels than wild-type cells. As determined using whole-cell fluorescence at 77 K, the photosystem I levels were also lower than those in wild-type cells. The BR2 mutant had significantly higher ROS levels compared to wild-type cells after exposure to high light for 30 min. Together, these results suggest that bilirubin plays an important role as a scavenger for ROS in Synechococcus sp. PCC 7002. The oxidation of bilirubin by ROS could convert bilirubin to biliverdin IXα, and thus BvdR might be important for regenerating bilirubin. These results further suggest that BvdR is a key component of a scavenging cycle by which cyanobacteria protect themselves from the toxic ROS byproducts generated during oxygenic photosynthesis.

3.
J Biol Chem ; 286(41): 35509-35521, 2011 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-21865169

RESUMO

When grown in green light, Fremyella diplosiphon strain UTEX 481 produces the red-colored protein phycoerythrin (PE) to maximize photosynthetic light harvesting. PE is composed of two subunits, CpeA and CpeB, which carry two and three phycoerythrobilin (PEB) chromophores, respectively, that are attached to specific Cys residues via thioether linkages. Specific bilin lyases are hypothesized to catalyze each PEB ligation. Using a heterologous, coexpression system in Escherichia coli, the PEB ligation activities of putative lyase subunits CpeY, CpeZ, and CpeS were tested on the CpeA and CpeB subunits from F. diplosiphon. Purified His(6)-tagged CpeA, obtained by coexpressing cpeA, cpeYZ, and the genes for PEB synthesis, had absorbance and fluorescence emission maxima at 566 and 574 nm, respectively. CpeY alone, but not CpeZ, could ligate PEB to CpeA, but the yield of CpeA-PEB was lower than achieved with CpeY and CpeZ together. Studies with site-specific variants of CpeA(C82S and C139S), together with mass spectrometric analysis of trypsin-digested CpeA-PEB, revealed that CpeY/CpeZ attached PEB at Cys(82) of CpeA. The CpeS bilin lyase ligated PEB at both Cys(82) and Cys(139) of CpeA but very inefficiently; the yield of PEB ligated at Cys(82) was much lower than observed with CpeY or CpeY/CpeZ. However, CpeS efficiently attached PEB to Cys(80) of CpeB but neither CpeY, CpeZ, nor CpeY/CpeZ could ligate PEB to CpeB.


Assuntos
Proteínas de Bactérias/metabolismo , Cianobactérias/enzimologia , Liases/metabolismo , Ficoeritrina/biossíntese , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Cianobactérias/genética , Liases/química , Liases/genética , Ficoeritrina/química , Ficoeritrina/genética
4.
Microbiol Resour Announc ; 11(12): e0087922, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36326505

RESUMO

We report the genome sequences of 24 newly discovered bacteriophages that infect Rhodobacter capsulatus, a model for photosynthesis and horizontal gene transfer studies. All have substantial relatedness to previously reported siphovirus bacteriophages. Most are categorized in known clusters (RcB, RcC, RcD, and RcF), with one forming a new cluster, RcG.

5.
J Bacteriol ; 193(7): 1663-71, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21296968

RESUMO

The pathway for phycocyanobilin biosynthesis in Synechococcus sp. strain PCC 7002 comprises two enzymes: heme oxygenase and phycocyanobilin synthase (PcyA). The phycobilin content of cells can be modified by overexpressing genes encoding alternative enzymes for biliverdin reduction. Overexpression of the pebAB and HY2 genes, encoding alternative ferredoxin-dependent biliverdin reductases, caused unique effects due to the overproduction of phycoerythrobilin and phytochromobilin, respectively. Colonies overexpressing pebAB became reddish brown and visually resembled strains that naturally produce phycoerythrin. This was almost exclusively due to the replacement of phycocyanobilin by phycoerythrobilin on the phycocyanin α-subunit. This phenotype was unstable, and such strains rapidly reverted to the wild-type appearance, presumably due to strong selective pressure to inactivate pebAB expression. Overproduction of phytochromobilin, synthesized by the Arabidopsis thaliana HY2 product, was tolerated much better. Cells overexpressing HY2 were only slightly less pigmented and blue-green than the wild type. Although the pcyA gene could not be inactivated in the wild type, pcyA was easily inactivated when cells expressed HY2. These results indicate that phytochromobilin can functionally substitute for phycocyanobilin in Synechococcus sp. strain PCC 7002. Although functional phycobilisomes were assembled in this strain, the overall phycobiliprotein content of cells was lower, the efficiency of energy transfer by these phycobilisomes was lower than for wild-type phycobilisomes, and the absorption cross-section of the cells was reduced relative to that of the wild type because of an increased spectral overlap of the modified phycobiliproteins with chlorophyll a. As a result, the strain producing phycobiliproteins carrying phytochromobilin grew much more slowly at low light intensity.


Assuntos
Proteínas de Bactérias/metabolismo , Ficobilinas/biossíntese , Ficobilinas/química , Synechococcus/enzimologia , Proteínas de Bactérias/genética , Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Estrutura Molecular , Mutagênese Insercional , Mutação , Synechococcus/citologia , Synechococcus/genética
6.
J Bacteriol ; 193(6): 1449-60, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21239582

RESUMO

Sulfur is an essential nutrient that can exist at growth-limiting concentrations in freshwater environments. The freshwater cyanobacterium Fremyella diplosiphon (also known as Tolypothrix sp. PCC 7601) is capable of remodeling the composition of its light-harvesting antennae, or phycobilisomes, in response to changes in the sulfur levels in its environment. Depletion of sulfur causes these cells to cease the accumulation of two forms of a major phycobilisome protein called phycocyanin and initiate the production of a third form of phycocyanin, which possesses a minimal number of sulfur-containing amino acids. Since phycobilisomes make up approximately 50% of the total protein in these cells, this elemental sparing response has the potential to significantly influence the fitness of this species under low-sulfur conditions. This response is specific for sulfate and occurs over the physiological range of sulfate concentrations likely to be encountered by this organism in its natural environment. F. diplosiphon has two separate sulfur deprivation responses, with low sulfate levels activating the phycobilisome remodeling response and low sulfur levels activating the chlorosis or bleaching response. The phycobilisome remodeling response results from changes in RNA abundance that are regulated at both the transcriptional and posttranscriptional levels. The potential of this response, and the more general bleaching response of cyanobacteria, to provide sulfur-containing amino acids during periods of sulfur deprivation is examined.


Assuntos
Cianobactérias/genética , Cianobactérias/metabolismo , Regulação Bacteriana da Expressão Gênica , Sulfatos/metabolismo , Transcrição Gênica , Ficobilissomas/metabolismo , Ficocianina/metabolismo , Estabilidade de RNA , RNA Bacteriano/metabolismo , RNA Mensageiro/metabolismo
7.
Biochemistry ; 50(22): 4890-902, 2011 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-21553904

RESUMO

Many cyanobacteria use brilliantly pigmented, multisubunit macromolecular structures known as phycobilisomes as antenna to enhance light harvesting for photosynthesis. Recent studies have defined the enzymes that synthesize phycobilin chromophores as well as many of the phycobilin lyase enzymes that attach these chromophores to their cognate apoproteins. The ability of the phycocyanin α-subunit (CpcA) to bind alternative linear tetrapyrrole chromophores was examined through the use of a heterologous expression system in Escherichia coli. E. coli strains produced phycocyanobilin, phytochromobilin, or phycoerythrobilin when they expressed 3Z-phycocyanobilin:ferredoxin oxidoreductase (PcyA), 3Z-phytochromobilin:ferredoxin oxidoreductase (HY2) from Arabidopsis thaliana, or phycoerythrobilin synthase (PebS) from the myovirus P-SSM4, respectively. CpcA from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002 was coexpressed in these strains with the phycocyanin α-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin α-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Both lyases were capable of attaching three different linear tetrapyrrole chromophores to CpcA; thus, up to six different CpcA variants, each with a unique chromophore, could be produced with this system. One of these chromophores, denoted phytoviolobilin, has not yet been observed naturally. The recombinant proteins had unexpected and potentially useful properties, which included very high fluorescence quantum yields and photochemical activity. Chimeric lyases PecE/CpcF and CpcE/PecF were used to show that the isomerizing activity that converts phycocyanobilin to phycoviolobilin resides with PecF and not PecE. Finally, spectroscopic properties of recombinant phycocyanin R-PCIII, in which the CpcA subunits carry a phycoerythrobilin chromophore, are described.


Assuntos
Proteínas de Bactérias/química , Escherichia coli/metabolismo , Ficocianina/química , Synechococcus/metabolismo , Proteínas de Bactérias/metabolismo , Liases/química , Liases/metabolismo , Ficocianina/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Synechococcus/química , Synechococcus/enzimologia
8.
Microbiol Resour Announc ; 10(29): e0055221, 2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34292064

RESUMO

Here, we report the genome sequence of bacteriophage Elbi, which infects the cyanobacterium Anabaena sp. strain PCC 7120, a model organism for prokaryotic multicellular development. The 68,626 bp encode 108 proteins, of which 31 can be assigned a function. Elbi is similar to two Anabaena myophages, namely, A-1 and N-1, isolated in the 1970s.

9.
PLoS One ; 16(11): e0255262, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34793465

RESUMO

The diversity of bacteriophages is likely unparalleled in the biome due to the immense variety of hosts and the multitude of viruses that infect them. Recent efforts have led to description at the genomic level of numerous bacteriophages that infect the Actinobacteria, but relatively little is known about those infecting other prokaryotic phyla, such as the purple non-sulfur photosynthetic α-proteobacterium Rhodobacter capsulatus. This species is a common inhabitant of freshwater ecosystems and has been an important model system for the study of photosynthesis. Additionally, it is notable for its utilization of a unique form of horizontal gene transfer via a bacteriophage-like element known as the gene transfer agent (RcGTA). Only three bacteriophages of R. capsulatus had been sequenced prior to this report. Isolation and characterization at the genomic level of 26 new bacteriophages infecting this host advances the understanding of bacteriophage diversity and the origins of RcGTA. These newly discovered isolates can be grouped along with three that were previously sequenced to form six clusters with four remaining as single representatives. These bacteriophages share genes with RcGTA that seem to be related to host recognition. One isolate was found to cause lysis of a marine bacterium when exposed to high-titer lysate. Although some clusters are more highly represented in the sequenced genomes, it is evident that many more bacteriophage types that infect R. capsulatus are likely to be found in the future.


Assuntos
Proteínas de Bactérias/genética , Bacteriófagos/genética , Regulação Bacteriana da Expressão Gênica , Variação Genética , Rhodobacter capsulatus/virologia , Técnicas de Transferência de Genes
10.
Appl Environ Microbiol ; 76(9): 2729-39, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20228104

RESUMO

Phycobiliproteins are water-soluble, light-harvesting proteins that are highly fluorescent due to linear tetrapyrrole chromophores, which makes them valuable as probes. Enzymes called bilin lyases usually attach these bilin chromophores to specific cysteine residues within the alpha and beta subunits via thioether linkages. A multiplasmid coexpression system was used to recreate the biosynthetic pathway for phycobiliproteins from the cyanobacterium Synechococcus sp. strain PCC 7002 in Escherichia coli. This system efficiently produced chromophorylated allophycocyanin (ApcA/ApcB) and alpha-phycocyanin with holoprotein yields ranging from 3 to 12 mg liter(-1) of culture. This heterologous expression system was used to demonstrate that the CpcS-I and CpcU proteins are both required to attach phycocyanobilin (PCB) to allophycocyanin subunits ApcD (alpha(AP-B)) and ApcF (beta(18)). The N-terminal, allophycocyanin-like domain of ApcE (L(CM)(99)) was produced in soluble form and was shown to have intrinsic bilin lyase activity. Lastly, this in vivo system was used to evaluate the efficiency of the bilin lyases for production of beta-phycocyanin.


Assuntos
Proteínas de Bactérias/biossíntese , Cianobactérias/metabolismo , Escherichia coli/metabolismo , Liases/metabolismo , Ficobiliproteínas/biossíntese , Synechococcus/enzimologia , Proteínas de Bactérias/metabolismo , Cianobactérias/enzimologia , Oxirredutases/metabolismo , Ficobilinas/metabolismo , Ficocianina/química , Ficocianina/metabolismo
11.
Arch Biochem Biophys ; 504(1): 86-99, 2010 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-20638360

RESUMO

Synechococcus sp. strain PCC 7002 is a robust, genetically tractable cyanobacterium that produces six different xanthophyll carotenoids (zeaxanthin, cryptoxanthin, myxoxanthophyll (myxol-2'-fucoside), echinenone, 3'-hydroxyechinenone, and synechoxanthin) and tolerates many environmental stresses, including high light intensities. Targeted mutations were introduced to block the branches of the carotenoid biosynthetic pathway leading to specific xanthophylls, and a mutant lacking all xanthophylls was constructed. Some of the mutants showed severe growth defects at high light intensities, and multi-locus mutants had somewhat lower chlorophyll contents and lower photosystem I levels. The results suggested that xanthophylls, particularly zeaxanthin and echinenone, might play regulatory roles in thylakoid biogenesis. Measurements of reactive oxygen (ROS) and nitrogen (RNS) species in the mutants showed that all xanthophylls participate in preventing ROS/RNS accumulation and that a mutant lacking all xanthophylls accumulated very high levels of ROS/RNS. Results from transcription profiling showed that mRNA levels for most genes encoding the enzymes of carotenogenesis are significantly more abundant after exposure to high light. These studies indicated that all xanthophylls contribute to protection against photo-oxidative stress.


Assuntos
Luz , Estresse Oxidativo/efeitos da radiação , Synechococcus/metabolismo , Synechococcus/efeitos da radiação , Xantofilas/metabolismo , Proliferação de Células/efeitos da radiação , Relação Dose-Resposta à Radiação , Perfilação da Expressão Gênica , Oxigenases de Função Mista/metabolismo , Mutação , Oxigenases/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Espectrometria de Fluorescência , Synechococcus/citologia , Synechococcus/genética , Temperatura , Xantofilas/biossíntese , Xantofilas/deficiência
12.
Adv Exp Med Biol ; 675: 211-28, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20532743

RESUMO

Cyanobacterial phycobiliproteins are brilliantly colored due to the presence of covalently attached chromophores called bilins, linear tetrapyrroles derived from heme. For most phycobiliproteins, these post-translational modifications are catalyzed by enzymes called bilin lyases; these enzymes ensure that the appropriate bilins are attached to the correct cysteine residues with the proper stereochemistry on each phycobiliprotein subunit. Phycobiliproteins also contain a unique, post-translational modification, the methylation of a conserved asparagine (Asn) present at beta-72, which occurs on the beta-subunits of all phycobiliproteins. We have identified and characterized several new families of bilin lyases, which are responsible for attaching PCB to phycobiliproteins as well as the Asn methyl transferase for beta-subunits in Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803. All of the enzymes responsible for synthesis of holo-phycobiliproteins are now known for this cyanobacterium, and a brief discussion of each enzyme family and its role in the biosynthesis of phycobiliproteins is presented here. In addition, the first structure of a bilin lyase has recently been solved (PDB ID: 3BDR). This structure shows that the bilin lyases are most similar to the lipocalin protein structural family, which also includes the bilin-binding protein found in some butterflies.


Assuntos
Cianobactérias/enzimologia , Liases/química , Liases/metabolismo , Ficobiliproteínas/biossíntese , Processamento de Proteína Pós-Traducional , Asparagina/metabolismo , Pigmentos Biliares/metabolismo , Cianobactérias/crescimento & desenvolvimento
13.
PLoS One ; 15(6): e0234636, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32555720

RESUMO

The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further explore this broad group of bacteriophages, we describe here the isolation and genomic characterization of 116 phages that infect Microbacterium spp. Most of the phages are lytic, and can be grouped into twelve clusters according to their overall relatedness; seven of the phages are singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp, and their G+C% content ranges from 51.4% to 71.4%, compared to ~67% for their Microbacterium hosts. The phages were isolated on five different Microbacterium species, but typically do not efficiently infect strains beyond the one on which they were isolated. These Microbacterium phages contain many novel features, including very large viral genes (13.5 kbp) and unusual fusions of structural proteins, including a fusion of VIP2 toxin and a MuF-like protein into a single gene. These phages and their genetic components such as integration systems, recombineering tools, and phage-mediated delivery systems, will be useful resources for advancing Microbacterium genetics.


Assuntos
Actinobacteria/virologia , Bacteriófagos/genética , Variação Genética , Genoma Viral , Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Composição de Bases , DNA Viral/genética , Genes Virais , Genômica , Filogenia , Proteínas Virais de Fusão/genética
14.
Mol Microbiol ; 68(2): 286-97, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18346116

RESUMO

Complementary chromatic adaptation (CCA) provides cyanobacteria with the ability to shift between red and blue-green phenotypes that are optimized for absorption of different wavelengths of light. Controlled by the ratio of green to red light, this process results from differential expression of two groups of operons, many of which encode proteins involved in photosynthetic light harvesting antennae biogenesis. In the freshwater species Fremyella diplosiphon, the inverse regulation of these two classes is complex and occurs through different mechanisms. It also involves a two-component pathway that includes a phytochrome-class photoreceptor and the response regulator RcaC. Here we uncover the mechanism through which this system controls CCA by demonstrating that RcaC binds to the L Box within promoters of both classes of light-regulated operons. We provide functional evidence that complementary regulation of these operons occurs by RcaC's simultaneous activation and repression of transcription in red light. We identify rcaC and L Boxes in the genome of a marine cyanobacterium capable of CCA, suggesting widespread use of this control system. These results provide important insights into the long-standing enigma of CCA regulation and complete the first description of an entire two-component system controlled by a phytochrome-class photoreceptor.


Assuntos
Proteínas de Bactérias/metabolismo , Cianobactérias/fisiologia , DNA Bacteriano/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Regiões Promotoras Genéticas , Fusão Gênica Artificial , Sequência de Bases , Sítios de Ligação , Pegada de DNA , DNA Bacteriano/genética , Ensaio de Desvio de Mobilidade Eletroforética , Genes Reporter , Glucuronidase/genética , Glucuronidase/metabolismo , Luz , Modelos Biológicos , Dados de Sequência Molecular , Ligação Proteica , Transdução de Sinais
15.
Nat Microbiol ; 2: 16251, 2017 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-28067906

RESUMO

Temperate phages are common, and prophages are abundant residents of sequenced bacterial genomes. Mycobacteriophages are viruses that infect mycobacterial hosts including Mycobacterium tuberculosis and Mycobacterium smegmatis, encompass substantial genetic diversity and are commonly temperate. Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct prophage-expressed viral defence systems that interfere with the infection of lytic and temperate phages that are either closely related (homotypic defence) or unrelated (heterotypic defence) to the prophage. Target specificity is unpredictable, ranging from a single target phage to one-third of those tested. The defence systems include a single-subunit restriction system, a heterotypic exclusion system and a predicted (p)ppGpp synthetase, which blocks lytic phage growth, promotes bacterial survival and enables efficient lysogeny. The predicted (p)ppGpp synthetase coded by the Phrann prophage defends against phage Tweety infection, but Tweety codes for a tetrapeptide repeat protein, gp54, which acts as a highly effective counter-defence system. Prophage-mediated viral defence offers an efficient mechanism for bacterial success in host-virus dynamics, and counter-defence promotes phage co-evolution.


Assuntos
Micobacteriófagos/fisiologia , Mycobacterium smegmatis/virologia , Mycobacterium tuberculosis/virologia , Prófagos/fisiologia , DNA Viral/genética , Variação Genética , Genoma Bacteriano , Genoma Viral , Ligases/genética , Lisogenia , Micobacteriófagos/genética , Mycobacterium smegmatis/genética , Mycobacterium tuberculosis/genética , Filogenia , Prófagos/enzimologia , Prófagos/genética , Proteínas Virais/genética
16.
Genome Biol ; 14(11): R127, 2013 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-24267588

RESUMO

BACKGROUND: 'Chlorochromatium aggregatum' is a phototrophic consortium, a symbiosis that may represent the highest degree of mutual interdependence between two unrelated bacteria not associated with a eukaryotic host. 'Chlorochromatium aggregatum' is a motile, barrel-shaped aggregate formed from a single cell of 'Candidatus Symbiobacter mobilis", a polarly flagellated, non-pigmented, heterotrophic bacterium, which is surrounded by approximately 15 epibiont cells of Chlorobium chlorochromatii, a non-motile photolithoautotrophic green sulfur bacterium. RESULTS: We analyzed the complete genome sequences of both organisms to understand the basis for this symbiosis. Chl. chlorochromatii has acquired relatively few symbiosis-specific genes; most acquired genes are predicted to modify the cell wall or function in cell-cell adhesion. In striking contrast, 'Ca. S. mobilis' appears to have undergone massive gene loss, is probably no longer capable of independent growth, and thus may only reproduce when consortia divide. A detailed model for the energetic and metabolic bases of the dependency of 'Ca. S. mobilis' on Chl. chlorochromatii is described. CONCLUSIONS: Genomic analyses suggest that three types of interactions lead to a highly sophisticated relationship between these two organisms. Firstly, extensive metabolic exchange, involving carbon, nitrogen, and sulfur sources as well as vitamins, occurs from the epibiont to the central bacterium. Secondly, 'Ca. S. mobilis' can sense and move towards light and sulfide, resources that only directly benefit the epibiont. Thirdly, electron cycling mechanisms, particularly those mediated by quinones and potentially involving shared protonmotive force, could provide an important basis for energy exchange in this and other symbiotic relationships.


Assuntos
Bactérias/genética , Genoma Bacteriano , Consórcios Microbianos/genética , Simbiose/genética , Bactérias/classificação , DNA Bacteriano/genética , Transferência Genética Horizontal , Genômica , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
17.
Nat Chem ; 4(5): 396-404, 2012 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-22522260

RESUMO

The effective absorption cross-section of a molecule (acceptor) can be greatly increased by associating it with a cluster of molecules that absorb light and transfer the excitation energy to the acceptor molecule. The basic mechanism of such light harvesting by Förster resonance energy transfer (FRET) is well established, but recent experiments have revealed a new feature whereby excitation is coherently shared among donor and acceptor molecules during FRET. In the present study, two-dimensional electronic spectroscopy was used to examine energy transfer at ambient temperature in a naturally occurring light-harvesting protein (PE545 of the marine cryptophyte alga Rhodomonas sp. strain CS24). Quantum beating was observed across a range of excitation frequencies. The shapes of those features in the two-dimensional spectra were examined. Through simulations, we show that two-dimensional electronic spectroscopy provides a probe of the adiabaticity of the free energy landscape underlying light harvesting.


Assuntos
Transferência Ressonante de Energia de Fluorescência , Complexos de Proteínas Captadores de Luz/efeitos da radiação , Fotossíntese , Transferência de Energia , Análise Espectral/métodos
18.
Methods Mol Biol ; 684: 273-93, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-20960136

RESUMO

Synechococcus sp. PCC 7002 is an ideal model cyanobacterium for functional genomics and biotechnological applications through metabolic engineering. A gene expression system that takes advantage of its multiple, endogenous plasmids has been constructed in this cyanobacterium. The method involves the integration of foreign DNA cassettes with selectable markers into neutral sites that can be located on any of the several endogenous plasmids of this organism. We have exploited the natural transformability and powerful homologous recombination capacity of this organism by using linear DNA fragments for transformation. This approach overcomes barriers that have made the introduction and expression of foreign genes problematic in the past. Foremost among these is the natural restriction endonuclease barrier that can cleave transforming circular plasmid DNAs before they can be replicated in the cell. We describe herein the general methodology for expressing foreign and homologous genes in Synechococcus sp. PCC 7002, a comparison of several commonly used promoters, and provide examples of how this approach has successfully been used in complementation analyses and overproduction of proteins with affinity tags.


Assuntos
Engenharia Genética/métodos , Plasmídeos/genética , Synechococcus/genética , Clonagem Molecular , Expressão Gênica , Vetores Genéticos/genética , Reação em Cadeia da Polimerase
19.
Mol Microbiol ; 64(2): 319-32, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17381552

RESUMO

Co-ordination of chromophore and apoprotein biosynthesis is required during photosynthetic light-harvesting antennae production, such as occurs during complementary chromatic adaptation (CCA). This response to ambient light colour changes is controlled by a phytochrome-class photoreceptor and involves changes in the synthesis of cyanobacterial light-harvesting antennae. During growth in red light, CCA activates cpc2 transcription, an operon that encodes the light-harvesting protein phycocyanin. In order to function, this apoprotein must have covalently attached phycocyanobilin chromophores, which are synthesized by PcyA. We show that pcyA is also transcriptionally activated by CCA during red light growth and is not regulated via feedback that senses cpc2 RNA levels. The pcyA and cpc2 promoters contain a common regulatory element, a direct repeat typical of OmpR-class transcription factor binding sites, at similar positions relative to their red light-controlled transcription start sites. Deletion of this element from the pcyA promoter eliminated CCA-regulated transcription, and insertion of the element into a non-light responsive promoter conferred CCA regulation. We conclude that this element is necessary and sufficient to confer CCA transcriptional regulation and that it co-ordinates phycocyanin and phycocyanobilin biosynthesis in red light.


Assuntos
Proteínas de Bactérias/genética , Cianobactérias/genética , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Complexos de Proteínas Captadores de Luz/genética , Luz , Ficobilinas/biossíntese , Ficocianina/biossíntese , Regiões Promotoras Genéticas/genética , Transativadores/genética , Adaptação Fisiológica/efeitos da radiação , Sequência de Bases , Análise por Conglomerados , Cianobactérias/efeitos da radiação , Retroalimentação Fisiológica/efeitos da radiação , Genes Bacterianos , Complexos de Proteínas Captadores de Luz/efeitos da radiação , Modelos Genéticos , Dados de Sequência Molecular , Oxirredutases/metabolismo , Ficobilissomas/metabolismo , Ficobilissomas/efeitos da radiação , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , Sequências Repetitivas de Ácido Nucleico/genética , Sítio de Iniciação de Transcrição , Transcrição Gênica/efeitos da radiação
20.
Plant Cell ; 15(10): 2448-63, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14508001

RESUMO

We have characterized the regulation of the expression of the pebAB operon, which encodes the enzymes required for phycoerythrobilin synthesis in the filamentous cyanobacterium Fremyella diplosiphon. The expression of the pebAB operon was found to be regulated during complementary chromatic adaptation, the system that controls the light responsiveness of genes that encode several light-harvesting proteins in F. diplosiphon. Our analyses of pebA mutants demonstrated that although the levels of phycoerythrin and its associated linker proteins decreased in the absence of phycoerythrobilin, there was no significant modulation of the expression of pebAB and the genes that encode phycoerythrin. Instead, regulation of the expression of these genes is coordinated at the level of RNA accumulation by the recently discovered activator CpeR.


Assuntos
Apoproteínas/genética , Cianobactérias/genética , Regulação Bacteriana da Expressão Gênica , Genes Sintéticos , Ficoeritrina/genética , Sequência de Aminoácidos , Apoproteínas/biossíntese , Apoproteínas/química , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Sequência de Bases , Cianobactérias/efeitos da radiação , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Luz , Dados de Sequência Molecular , Mutagênese , Ficoeritrina/biossíntese , Ficoeritrina/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico
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