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1.
Eur J Nutr ; 52(2): 799-812, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22684609

RESUMO

PURPOSE: To identify the key parameters involved in cereal starch digestion and associated glycaemic response by the utilisation of a dynamic gastro-duodenal digestion model. METHODS: Potential plasma glucose loading curves for each meal were calculated and fitted to an exponential function. The area under the curve (AUC) from 0 to 120 min and total digestible starch was used to calculate an in vitro glycaemic index (GI) value normalised against white bread. Microscopy was additionally used to examine cereal samples collected in vitro at different stages of gastric and duodenal digestion. RESULTS: Where in vivo GI data were available (4 out of 6 cereal meals) no significant difference was observed between these values and the corresponding calculated in vitro GI value. CONCLUSION: It is possible to simulate an in vivo glycaemic response for cereals when the gastric emptying rate (duodenal loading) and kinetics of digestible starch hydrolysis in the duodenum are known.


Assuntos
Duodeno/metabolismo , Grão Comestível/química , Índice Glicêmico , Amido/metabolismo , Adolescente , Adulto , Idoso , Área Sob a Curva , Glicemia/análise , Gorduras na Dieta/análise , Proteínas Alimentares/análise , Digestão/fisiologia , Jejum , Manipulação de Alimentos , Humanos , Concentração de Íons de Hidrogênio , Hidrólise , Processamento de Imagem Assistida por Computador , Refeições , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Modelos Biológicos , Polissacarídeos/análise , Viscosidade , Adulto Jovem , beta-Glucanas/análise
2.
Appl Spectrosc ; 76(5): 559-568, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35216528

RESUMO

Raman spectroscopy is a viable tool within process analytical technologies due to recent technological advances. In this article, we evaluate the feasibility of Raman spectroscopy for in-line applications in the food industry by estimating the concentration of the fatty acids EPA + DHA in ground salmon samples (n = 63) and residual bone concentration in samples of mechanically recovered ground chicken (n = 66). The samples were measured under industry like conditions: They moved on a conveyor belt through a dark cabinet where they were scanned with a wide area illumination standoff Raman probe. Such a setup should be able to handle relevant industrial conveyor belt speeds, and it was studied how different speeds (i.e., exposure times) influenced the signal-to-noise ratio (SNR) of the Raman spectra as well as the corresponding model performance. For all samples we applied speeds that resulted in 1 s, 2 s, 4 s, and 10 s exposure times. Samples were scanned in both heterogenous and homogenous state. The slowest speed (10 s exposure) yielded prediction errors (RMSECV) of 0.41%EPA + DHA and 0.59% ash for the salmon and chicken data sets, respectively. The more in-line relevant exposure time of 1 s resulted in increased RMSECV values, 0.84% EPA + DHA and 0.84% ash, respectively. The increase in prediction error correlated closely with the decrease in SNR. Further improvements of model performance were possible through different noise reduction strategies. Model performance for homogenous and heterogenous samples was similar, suggesting that the presented Raman scanning approach has the potential to work well also on intact heterogenous foods. The estimation errors obtained at these high speeds are likely acceptable for industrial use, but successful strategies to increase SNR will be key for widespread in-line use in the food industry.


Assuntos
Salmão , Análise Espectral Raman , Animais , Estudos de Viabilidade , Indústria Alimentícia , Análise Espectral Raman/métodos
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