Cell tropism and molecular epidemiology of Anaplasma platys-like strains in cats.

Bacterial species of the genus Anaplasma are tick transmitted pathogens that negatively impact on animal productions and generate veterinary and public health concerns. This paper reports the identification, molecular characterization and phylogeny of novel unclassified A. platys-like strains in cats. Interestingly, these novel strains are closely related to conspecific strains recently identified in ruminants, and significantly differ from A. platys. A. platys-like strains in cats, unlike ruminants strains, show tropism for platelets. Results have implications in the diagnostic scenario of animal anaplasmosis and provide background for reconstructing the evolutionary history of species genetically related to A. platys.

Delayed development and rupture of an aortic aneurysm after closed abdominal trauma.

A case is reported of a patient who, following the surgical repair of a lesion of the superior mesenteric artery secondary to a closed abdominal trauma, slowly developed an aneurysm of the abdominal aorta which subsequently ruptured. Surgical management of the aneurysm by aortic graft implantation proved successful.

Combined laparoendoscopic approach for biliary lithiasis treatment.

BACKGROUND/AIMS: The treatment of common bile duct stones diagnosed during videolaparoscopic cholecystectomy is still under debate. In cases of suspected common bile duct stones, a double approach with endoscopic retrograde cholangiopancreatography either prior to, or following videolaparoscopic cholecystectomy is the current routine in many centers. An intraoperative endoscopic retrograde cholangiopancreatography with endoscopic papillosphincterotomy and stone extraction has recently been proposed. METHODOLOGY: We compared the approaches for suspected common bile duct stones in 21 cases of combined intervention endoscopic retrograde cholangiopancreatography during videolaparoscopic cholecystectomy to 17 cases of sequential intervention (endoscopic retrograde cholangiopancreatography prior to videolaparoscopic cholecystectomy). Complications and postoperative monitoring are discussed and reported on the basis of hospital stay. RESULTS: Although the efficacy and the complications are similar, patients treated with the sequential approach stayed in the hospital longer because of the double monitoring period during both after endoscopic retrograde cholangiopancreatography and after videolaparoscopic cholecystectomy. CONCLUSIONS: A combined approach to suspected common bile duct stones during videolaparoscopic cholecystectomy could be an effective and a financially worthwhile treatment.

[The role and limitations of litholytic therapy of cholesterin cholecystolithiasis. Results in 86 cases treated with chenic acid]. / Ruolo e limiti della terapia litolitica della colecistolitiasi colesterinica. Risultati di 86 casi trattati con acido chenico.

86 carefully selected patients with cholesterinic cholecystic lithiasis were submitted to litholytic treatment with chenodesoxycholic acid at a dose of 15 mg per kg of body weight as part of a multidisciplinary therapeutic approach to biliary lithiasis. Follow up of these patients up to 24 months showed the positive action of the drug on dyspeptic pain symptomatology, the absence of significant side effects, and the absence of hepatotoxic effects of chenic acid at therapeutic doses, as well as its effectiveness with respect to total or partial litholysis in 78% of patients. These data confirm the positive role of litholytic treatment as an alternative to cholecystectomy in highly selected patients treated at specialist centres.

[Experience with the treatment of acute hepatic coma using extracorporeal perfusion of heterologous liver and exchange transfusion]. / Esperienza di trattamento del coma epatico acuto mediante perfusione extracorporea di fegato eterologo ed exsanguinotrasfusione

Extracorporeal pig liver perfusion was adopted in 2 patients with acute hepatic coma due to fulminant hepatitis. Exchange transfusion was used in a third patient, while a fourth was subjected to both procedures. A marked haematological improvement was noted in all cases. Yet, three patients died without showing any clinical benefit. In the fourth case, coma regressed and gradual improvement to the point of complete recovery was achieved. Liver perfusion and exchange transfusion in the same patient were attended by very different clinical effects, though each procedure produced a comparable improvement at the blood chemistry data. Careful analysis of the data suggests that the difference was attributable to discrepancies between the degree of tissue purification achieved.

Clinical and therapeutic applications of a new pathogenetic theory on the dumping syndrome.

Based on experimental and clinical studies we have deduced the existence of a neuro-osmotic dumping syndrome and a truly osmotic dumping syndrome that differ in pathogenesis and clinical symptoms.

Rational indications for sphincterotomy through intraoperative cholangiomanometry.

The loss of function of Oddi's sphincter due to sclerosis secondary to a lithiasic or alithiasic phlogosis, is the only rational indication for sphincterotomy. An operative test of the Oddi sphincter function is proposed, based on an original manometric procedure. This method, employed on 567 patients undergoing biliary surgery, allowed demonstration of a loss of function of Oddi's sphincter in 53 patients (9.4%), in whom a sphincterotomy was performed.

[Pseudoaneurysm of the right iliac artery with fistula into the cecum. A clinical case]. / Pseudoaneurisma dell'arteria iliaca destra fistolizzato nel cieco. Osservazione clinica.

A case is reported of pseudoaneurysm of the right iliac artery secondary to an endarterectomy with dacron patch due to atherosclerosis performed 4 years ago. The case presented acute anemia and massive enteric hemorrhage subsequent to arterial rupture into the cecum. Because of the dramatic onset, the patient was immediately operated. The surgical procedure used was total pseudoaneurysm excision, "in situ" by-pass with dacron prosthesis and suture of the cecum. The problems of pathophysiology, diagnosis, symptoms and management are discussed and compared to previously published cases. The peculiarity of the described case is underlined.

[Neurotoxicity of intra-arterial cis-platinum (CDDP) in the locoregional treatment of diffuse liver metastasis of carcinoma of the colon and rectum]. / Neurotossicità del cis-platino (CDDP) intra-arterioso nel trattamento locoregionale delle metastasi epatiche diffuse da carcinoma del colon-retto.

Peripheral neurotoxicity of i.v. cisplatin (CDDP) at high cumulative dosage is well documented. On the contrary neurotoxicity of CDDP following intra-arterial administration is less known. Five patients with liver metastasis from colorectal carcinoma have been treated with intraarterial CDDP and i.v. 5-fluorouracil according to a trial of the National Registry on Implantable Devices (RNSI); in these patients a severe peripheral neurotoxicity has been observed since the fourth cycle of chemotherapy. In the present paper we report the electroneurographic changes observed immediately after chemotherapy and several months later. Clinical characteristics of neuropathy following i.a. CDDP are the following: preferentially localized at the distal extremities of the limbs, exclusively sensitive, not reversible.

[New method for preparation of the totally hepatectomized dog. Critical review of the literature and surgical technics]. / Nuovo metodo per la preparazione del cane totalmente epatectomizzato. Rassegna critica della letteratura e tecnica chirurgica

A critical review of the relevant literature is followed by the presentation of a new method for total hepatectomy in the dog. This is carried out in a single stage. Its main advantage is there is no temporary interruption of the portal or portocaval flow. A synthetic prosthesis is anastomosed end-to-end with the subhepatic vena cava and end-to-side with the supradiaphragmatic cava. Next, the portal vein is anastomosed side-to-side with the prosthesis and ligated upstream. Hepatectomy is then performed.

[Selective anterior vagotomy of the duodenum. Propositions, technics and experimental results]. / La vagotomia selettiva anteriore del duodeno. Premesse, tecnica e risultati sperimentali

Previous studies have demonstrated that changes in endoduodenal pressure may cause pancreatic alterations in biliary diseases. Anterior selective vagotomy of the duodenum was devised to reduce duodenal motility. An experimental study showed that such a surgical procedure is followed by a marked decrease in the overall duodenal kinesis, with disappearance of the highest pressure peaks.

[Extended and palliative surgery in the treatment of gastric cancer in advanced stage]. / Chirurgia allargata e palliativa nel trattamento del cancro gastrico in fase avanzata.

Five hundred and fifty patients operated between 1965 and 1983 for stomach carcinoma were included in the study. Of these, 244 (44.4%) underwent extended (54 patients) or palliative surgery (190 patients) due to the extragastric diffusion of the neoplasia. Global postoperative mortality for patients undergoing extended surgery was 16.7% and the five-year survival rate was 18.5%; in the case of those undergoing palliative surgery the postoperative mortality rate was 30.5% and the two-year survival rate was 14% for resection and 4% for derivatives. Patients who underwent a derivative operation showed an easing of symptoms and an improved quality of life. On the basis of these results the Authors consider that the possibility of extended surgery should still be carefully evaluated in patients in whom a stomach tumour has exceeded the gastric boundary, irrespective of its extent, since the limit of radical surgery is not related to the extension of surgical demolition but to the entity of the extragastric diffusion of the neoplasia. In addition, surgical abstention is not always justified even in patients in whom surgery cannot be curative since a 10% five-year survival rate was observed in patients undergoing palliative resection.

[Surgery of biliary lithiasis. I. Physiopathology and diagnosis]. / La chirurgia della litiasi biliare. Nota I. Fisiopatologia e diagnostica.

Experimental studies and clinical observations are referred to in stressing the frequent correlation between duodenal, biliary and pancreatic disturbances, and the importance of the role of spontaneous or iatrogenic impairment of Oddi's sphincter in the origin of this pathology. These physiopathological concepts are regarded as fundamental in the correct surgical management of cholelithiasis. A pre- and postoperative diagnostic protocol designed to investigate the anatomofunctional aspects of the duodenum and pancreas as well as bile duct morphology and Oddi sphincter function is described. Its employment enabled 633 cases to be divided six well-defined groups for diversified treatment.

[Surgery of biliary lithiasis. II. Choice of operation and results]. / La chirurgia della litiasi biliare. Nota II. Scelta dell'intervento e risultati.

A pre-and intraoperative diagnostic protocol offering morphological and functional evaluation of the bile ducts, Oddi's sphincter, duodenum, and pancreas was employed to classify 633 patients with biliary lithiasis into 6 groups and hence the therapeutic application of 6 surgical techniques, namely: 1) cholecystectomy alone; 2) choledocholithotomy plus closure of the choledoch; 3) choledocholithotomy plus Kehr drainage; 4) papillosphincterotomy alone; 5) papillosphincterotomy plus anterior seclective duodenal vagotomy; 6) hepaticojejunostomy, so as to ensure a rational choice of the best operation fro each situation. Comparison with the literature and prior personal results substantiated the soundness of this approach, as shown by a marked reduction of complications, mortality and residual disease on both short and medium-term follow-up.

[Short and median term results of 234 cases of gastrectomy with the Billroth II method. Critical evaluation]. / Risultati a breve e medio termine su 234 casi di resezioni gastriche secondo B2. Valutazione critica.

234 partial gastrectomies, according to Billroth II technique, were performed for peptic ulcer disease in selective patients from 1969 to 1977. A postoperative study has been carried out to check the efficiency of such operation in the treatment of peptic ulcer disease. Postoperative treatment of peptic ulcer disease. Postoperative mortality was 3.8%, while immediate surgical complications were 6.4%. 145 of this patients could be followed with a special regard for sex, age, way of living, general conditions, ulcer's location and the persisting symptoms at the time of the study. 124 patients (84%) showed good general conditions while 6 (4.1%) needed a second operation for recurrent peptic ulceration or alkaline reflux gastritis. Although this procedure has showed fairly good clinical results the Authors point out the high rate of postoperative mortality for a basically benign disease and the presence of some unpleasant digestive sequelae. Attention is payed to some factors that may play an important role on the pathophysiology of these sequelae.

[Success in a case of fulminant hepatitis treated with extracorporeal liver perfusion and exchange transfusion]. / Successo in un caso di epatite fulminante trattato con perfusione di fegato extracorporeo ed exsanguinotrasfusione

Extracorporeal perfusion of pig liver lasting 4 hr, followed by isovolaemic exchange (6 litres in 1 hr) were employed in the management of coma in a case of acute virus hepatitis. Liver perfusion led to temporary regression of coma, whereas transfusion was followed by gradual improvement in the clinical picture; this was apparently not a direct consequence of the procedure adopted. Assessment of the degree of purification obtained with these two methods offers an explanation for the difference in their clinical effectiveness.

[Distal gastrectomy with Y gastroenteroanastomosis not associated with vagotomy in elective surgical treatment of gastroduodenal ulcer. Clinical and functional long-term results]. / La gastrectomia distale con gastroenteroanastomosi a Y non associata a vagotomia nel trattamento chirurgico di elezione dell'ulcera gastroduodenale. Risultati clinici e funzionali a lungo termine.

During the period 1977-1984, 53 patients with peptic ulcer resistant to H2-blockers (29 gastric and 24 duodenal ulcers) were submitted to distal gastrectomy with Roux-en-Y gastroenteroanastomosis not associated with vagotomy. The indication was used as an alternative to proximal vagotomy in cases with delayed gastric emptying, high acid output, perforation or bleeding. The study plan consisted of serial clinical and instrumental controls including determination of basal (BAO) or maximal (MAO) acid output. Operative mortality was nil. At various times after the operation, 4 patients died for unrelated reasons and 3 were lost to follow-up. Median follow-up was 84 months with an interval of from 4 to 137 months. Fifty-one patients were followed up for at least one year and 49 for at least two. Five patients (2 gastric and 3 duodenal ulcers) developed peptic recurrences (Visick IV, 9.8%) by the end of the first postoperative year and, in all cases but one, healed stably by the second year after medical (3 cases) or surgical (1 case) therapy. In the remaining patients, Visick was grade III in 6 cases (4 gastric and 2 duodenal ulcers), grade II in 6 and grade I in 34. Before operation, mean values (+/- SD) of BAO and MAO were respectively 5.84 +/- 5.03 and 29.6 +/- 18.6 mEq/h. In the immediate postoperative period there was a considerable reduction in BAO (p less than 0.02) and MAO (p less than 0.03) which continued up to the third postoperative year with a tendency to further progressive reduction in MAO. In spite of a considerable individual variability in dimensions and temporal evolution, the phenomenon occurred qualitatively in all cases. No significant difference was observed in the behaviour of BAO and MAO in gastric ulcers by comparison with duodenal ulcers and in cases with recurrence and Visick III compared to those with a favourable clinical result.

[Evaluation of the functional modifications of the lower esophageal sphincter after use of the Angelchik prosthesis]. / Valutazione delle modificazioni funzionali dello sfintere esofageo inferiore (LES) dopo applicazione della protesi di Angelchik.

The manometric recording of Lower Esophageal Sphincter (LES) has been made in 8 patients who have undergone surgical apposition of Angelchik prosthesis for hiatal hernia. The patients underwent an esophageal manometric study in the preoperative period, one month and one year after the intervention. In the preoperative patients, the mean LES pressure was 10,3 +/- 0,5 mmHg and there was sphincterial incoordination to peristaltic waves after swallow. In one month postoperative patients the LESP was 20,1 +/- 1,0 mmHg (p less than 0,001), and one year after the LESP was 17,0 +/- 0,3 (p less than 0,001) with an ameliorated coordination of the LES activity in response to swallow. Therefore, during postoperative manometric controls the Authors identified a high pressure subdiaphragmatic zone unmodified by deglutitory acts which was not present in the preoperative investigation. Probably the presence of this zone would been explained as a consequence of mechanical action of the prosthesis and the postsurgical fibrino-reaction around the prosthesis. The Authors discussed the results obtained and their importance to Angelchik prosthesis employment for surgical correction of hiatal hernia.

[Degree of parietal penetration of gastric cancer in relation to medium- and long-term survival]. / Il grado di penetrazione parietale del cancro gastrico in relazione alla sopravvivenza a medio e lungo termine.

The Authors analysed their experience about gastric carcinoma, with particular reference to short, middle and long time survival in relation to the degree of parietal penetration. In the patients without remote metastases, the survival till 5 years depends mainly on this factor, whereas the lymphonodal spreading plays a secundary rôle, especially in the cases where the tumour deeply infiltrated the gastric wall. Lymphoadenectomy, therefore, seems essential in cases of neoplasm with low degree of parietal penetration, whereas in those with high degree it does not seem determinant on the remote results.

[Validity of distal subtotal gastroresection in the treatment of tumors of the lower 1/3 of the stomach]. / Validità della gastroresezione sub-totale distale nel trattamento dei tumori del 1/3 inferiore dello stomaco.

The Authors analysed the results of a casuistry of 75 patients subjected to radical subtotal gastroresection for carcinoma of the distal third of stomach, with the purpose to verify the validity of such operation, as compared to the total gastrectomy on principle. The postoperative mortality reached 8% and morbidity 8.7%. The survival after 5 years reached 42%. 85% of the patients, at checkings, resulted in good or excellent conditions (Visik 1-2). On the basis of these data, the Authors conclude the distal subtotal gastric resection should be considered the choice treatment for tumours of the lower third of stomach.