RESUMO
A novel engineering methodology for organizing a large liver tissue equivalent was established by intergrating both 'top down' and 'bottom up' approaches. A three-dimensional (3D) scaffold was engineered comprising 43 culture chambers (volume: 11.63 cm(3)) assembled in a symmetrical pattern on 3 layers, a design which enables further scaling up of the device to a clinically significant size (volume: 500 cm(3)). In addition, an inter-connected flow channel network was designed and proved to homogenously deliver culture medium to each chamber with the same pressure drop. After fabrication using nylon-12 and a selective laser sintering process, co-cultured cellular aggregates of human hepatoma Hep G2 and TMNK-1 cells were loosely packed into the culture chambers with biodegradable poly-L-lactic acid fibre pieces for 9 days of perfusion culture. The device enabled increased hepatic function and well-maintained cell viability, demonstrating the importance of an independent medium flow supply for cell growth and function provided by the current 3D scaffold. This integrative methodology from the macro- to the micro-scale provides an efficient way of arranging engineered liver tissue with improved mass transfer, making it possible to further scale up to a construct with clinically relevant size while maintaining high per-volume-based physiological function in the near future.
Assuntos
Técnicas de Cultura de Células/métodos , Fígado Artificial , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Albuminas/metabolismo , Técnicas de Cultura de Células/instrumentação , Linhagem Celular , Sobrevivência Celular , Técnicas de Cocultura , Desenho Assistido por Computador , Glucose/metabolismo , Células Hep G2 , Humanos , Modelos Biológicos , Poliésteres/química , Engenharia Tecidual/instrumentaçãoRESUMO
The role of cAMP in regulation of intracellular pH in the confluent LLC-PK1 cells was investigated. DibutyrylcAMP and forskolin induce intracellular acidification. This acidification is inhibited by DIDS and ethacrynic acid, inhibitors of Na(+)-independent Cl-/HCO3- exchange, and by removal of extracellular Cl-. In addition, Bt2 cAMP causes Cl- entry into LLC-PK1 cells. These results suggest that cAMP activates Cl- transport, namely Na(+)-independent Cl-/HCO3- exchange, which participates in pHi regulation.
Assuntos
Proteínas de Transporte/metabolismo , Cloretos/metabolismo , AMP Cíclico/metabolismo , Rim/metabolismo , Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico , Ácido 4-Acetamido-4'-isotiocianatostilbeno-2,2'-dissulfônico/análogos & derivados , Ácido 4-Acetamido-4'-isotiocianatostilbeno-2,2'-dissulfônico/farmacologia , Amilorida/farmacologia , Animais , Bicarbonatos/metabolismo , Bucladesina/farmacologia , Linhagem Celular , Antiportadores de Cloreto-Bicarbonato , Colforsina/farmacologia , Células Epiteliais , Epitélio/metabolismo , Ácido Etacrínico/farmacologia , Furosemida/farmacologia , Concentração de Íons de Hidrogênio , Líquido Intracelular/efeitos dos fármacos , Líquido Intracelular/metabolismo , Rim/citologia , Suínos , Fatores de Tempo , Ácido Trinitrobenzenossulfônico/farmacologiaRESUMO
The effect of human adrenomedullin on cerebral circulation was investigated in dogs in vivo and in vitro. Bolus administration of adrenomedullin or its homologous peptides, calcitonin gene-related peptide (CGRP) and amylin, into the vertebral artery induced a dose-dependent increase in vertebral blood flow. The potencies of adrenomedullin and CGRP were similar and approximately 100 times more than that of amylin. The effects of adrenomedullin and CGRP were inhibited by CGRP8-37, an antagonist of CGRP. In contrast to substance P, adrenomedullin did not induce an increase in blood flow after prior administration of CGRP. Pretreatment with either NG-nitro-L-arginine methyl ester or indomethacin did not affect the adrenomedullin-induced increase in blood flow. Intracisternal administration of adrenomedullin induced dilation of the basilar and other major cerebral arteries in a dose-dependent manner, accompanied by an increase in the concentration of cyclic AMP in the cerebrospinal fluid. Adrenomedullin also induced relaxation of isolated basilar and middle cerebral arterial rings. These data suggest that adrenomedullin induces vasodilation of cerebral arteries and an increase in vertebral blood by acting at CGRP receptors positively coupled to adenylate cyclase, and that these effects are not dependent on nitric oxide or prostaglandin formation.
Assuntos
Amiloide/farmacologia , Peptídeo Relacionado com Gene de Calcitonina/farmacologia , Circulação Cerebrovascular/efeitos dos fármacos , Peptídeos/farmacologia , Adrenomedulina , Animais , Anti-Hipertensivos/farmacologia , Angiografia Cerebral , Artérias Cerebrais/efeitos dos fármacos , AMP Cíclico/metabolismo , Cães , Relação Dose-Resposta a Droga , Feminino , Humanos , Polipeptídeo Amiloide das Ilhotas Pancreáticas , Masculino , Concentração Osmolar , Vasodilatadores/farmacologia , Artéria Vertebral/diagnóstico por imagem , Artéria Vertebral/fisiologiaRESUMO
One of the limitations of immunoperoxidase methods applied in surgical pathology is the loss of immunoreactivity during fixation and processing. In this study, the effects of decalcification on the immunoreactivity of various antigens in formalin fixed tissues were examined. The pieces of fixed tissue were placed in four different decalcifying solutions (EDTA, formic acid, nitric acid, and Plank-Rychlo solution) for various time periods and then processed routinely and embedded in paraffin. The paraffin sections of the tissues were stained with immunoperoxidase methods for immunoglobulins, hormones, or tissue/tumor-specific markers. Sections of nondecalcified tissues were used as a control. The immunohistochemical staining intensity and the number of positive cells were not reduced significantly in decalcified tissues within the usual time period of routine decalcification procedures. Many antigens survived even prolonged decalcification. The results of this study indicate that routinely decalcified tissues can be used for immunoperoxidase staining without significant loss of immunoreactivity.
Assuntos
Técnica de Descalcificação , Técnicas Imunoenzimáticas , Coloração e Rotulagem , Fixadores , Histocitoquímica , Técnicas Histológicas , Humanos , ImunoquímicaRESUMO
Thrombopoiesis, as well as antibody production, is one of the major events in which interleukin-6 (IL-6) has been reported to be involved. Polyclonal anti-murine IL-6 receptor antibody was prepared to examine the effect of the antibody on these events in IL-6-treated mice. Administration of the anti-mIL-6R antibody inhibited the IL-6-induced increase in the number of platelets. Enhancement of the serum level of DNP-specific antibody by intraperitoneal injection of IL-6 was inhibited completely with simultaneous administration of the anti-mIL-6R antibody. The level of DNP-specific antibody was decreased, even below the basal value, by the higher dose of anti-mIL-6R antibody, indicating its effect also on endogenous IL-6. This work provides evidence that anti-IL-6R antibody inhibits IL-6 function in vivo, and provides an animal model of the therapeutic use of anti-IL-6R antibody for IL-6-related disease.
Assuntos
Interleucina-6/antagonistas & inibidores , Receptores Imunológicos/imunologia , Animais , Anticorpos/fisiologia , Plaquetas/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Dinitrofenóis , Modelos Animais de Doenças , Cobaias , Haptenos/efeitos dos fármacos , Interleucina-6/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Contagem de Plaquetas , Receptores de Interleucina-6 , Proteínas Recombinantes/farmacologia , Soroalbumina Bovina/efeitos dos fármacos , Células Tumorais CultivadasRESUMO
The Kir6.1/uK(ATP)-1, subunit of ATP-sensitive K(+) channels (K(ATP)), was localized in adult rat brain by in situ hybridization and immunohistochemistry. The mRNA of this subunit was ubiquitously expressed in various neurons and nuclei of the adult rat brain. Interestingly, Kir6.1/uK(ATP)-1 mRNA was also expressed in glial cells. Kir6.1/uK(ATP)-1 protein staining gave a dispersed array of fine dots throughout all neurons and glial cells examined. Under electron microscope, the immunoreactive products were specifically restricted to the mitochondria. The present study indicates that this K(ATP) subunit is localized in the mitochondria and may play a fundamental role in vital brain function.
Assuntos
Trifosfato de Adenosina/fisiologia , Encéfalo/metabolismo , Canais de Potássio/genética , Animais , Encéfalo/ultraestrutura , Química Encefálica , Cerebelo/química , Cerebelo/metabolismo , Cerebelo/ultraestrutura , Diencéfalo/química , Diencéfalo/metabolismo , Imuno-Histoquímica , Hibridização In Situ , Masculino , Bulbo/química , Bulbo/metabolismo , Mesencéfalo/química , Mesencéfalo/metabolismo , Microscopia Imunoeletrônica , Mitocôndrias/química , Mitocôndrias/ultraestrutura , Bulbo Olfatório/química , Bulbo Olfatório/metabolismo , Ponte/química , Ponte/metabolismo , Canais de Potássio/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos WistarRESUMO
The vasorelaxant effects of pituitary adenylate cyclase activating polypeptide (PACAP)-27 were examined and compared with those of PACAP-38 and vasoactive intestinal polypeptide (VIP) on isolated canine cerebral arteries and rat intracerebral arterioles in vitro. The addition of PACAP-27, PACAP-38 or VIP resulted in similar concentration-dependent relaxations in both canine basilar arteries and rat intracerebral arterioles. There were regional differences in the PACAP-27-induced relaxations measured in canine cerebral arteries. The maximum relaxation induced by PACAP-27 was significantly lower in the basilar arteries (23.0 +/- 5.6%) than in the rostrally located arteries (proximal middle cerebral arteries: 45.4 +/- 5.7%, anterior cerebral arteries: 55.2 +/- 5.8%). The maximum relaxation induced by PACAP-27 in the basilar arteries was significantly enhanced by mechanical removal of the endothelium (16.4 +/- 4.5% vs. 32.7 +/- 5.8%) as well as by pretreatment with indomethacin or aspirin (12.9 +/- 4.1% vs. 48.7 +/- 6.1% and 46.5 +/- 9.2%, respectively). Incubation of canine cerebral arteries with PACAP-27 in vitro resulted in an increased release of prostaglandin F2 alpha in the buffer from 14.5 +/- 2.1 pg/min/1 mg vessel to 31.1 +/- 4.2 pg/min/1 mg vessel, while other cyclooxygenase cascade metabolites such as prostaglandin E2, thromboxane B2 and 6-keto prostaglandin F1 alpha did not change. These data suggest that the PACAP-27-induced relaxation of canine basilar arteries may be associated with prostaglandin F2 alpha or its precursor, prostaglandin H2.
Assuntos
Músculo Liso Vascular/efeitos dos fármacos , Neuropeptídeos/farmacologia , Vasodilatadores/farmacologia , Animais , Arteríolas/efeitos dos fármacos , Artéria Basilar/efeitos dos fármacos , Artéria Basilar/metabolismo , Artérias Cerebrais/efeitos dos fármacos , Artérias Cerebrais/metabolismo , Cães , Endotelinas/farmacologia , Feminino , Humanos , Técnicas In Vitro , Masculino , Relaxamento Muscular/efeitos dos fármacos , Músculo Liso Vascular/metabolismo , Polipeptídeo Hipofisário Ativador de Adenilato Ciclase , Antagonistas de Prostaglandina/farmacologia , Prostaglandinas/metabolismo , Ratos , Ratos Sprague-Dawley , Peptídeo Intestinal Vasoativo/farmacologiaRESUMO
To better assess the efficacy of mechanical preparation of root canals, transparent specimens of 510 extracted maxillary central incisors were investigated for thickness and curvature of the root canal, condition of any accessory canals, and location of the apical foramen. Over 60% of the specimens showed accessory canals that are impossible to clean mechanically. Most lateral branches were small, 80% were the size of a #10 reamer or less, and only 3% were thicker than a #40 reamer. Apical foramina located away from the apex were observed in 45% of the teeth, although nearly 80% of all foramina were within approximately 0.5 mm of the apex, and 95% were within approximately 1.0 mm. Data on the thickness and curvature of the main canal showed that normally it is adequately prepared when reached with a #60 reamer to the apical constriction and supplemented by flare preparation.
Assuntos
Cavidade Pulpar/anatomia & histologia , Incisivo/anatomia & histologia , Raiz Dentária/anatomia & histologia , Humanos , Maxila , Odontometria , Tratamento do Canal RadicularRESUMO
BACKGROUND: Trichoblastoma is a rare benign skin appendage tumor constituted mostly of follicular germinative cells. It can arise on any part of the body except the palms, soles, nail units and mucosal membranes. No case of it in breast skin has been reported before. Furthermore, fine needle aspiration cytology findings on this lesion have not been described before. CASE: A 76-year-old female presented with a firm nodule in her left breast. The tumor was well demarcated, about 1.5 cm in diameter. Fine needle aspiration cytology revealed clusters composed of relatively uniform cells with a high nuclear/cytoplasmic ratio. In the midst of some clusters, the tumor cells had a more abundant cytoplasm. Fibrocellular interstitium or dense cyanophilic acellular material occasionally was attached to them. The tumor cells had oval or fusiform nuclei that had fine, evenly dispersed chromatin. To exclude a diagnosis of breast cancer, it is important to recognize that the clusters are composed of basaloid cells with focal squamous eddies and that there is at least focally peripheral palisading. The histopathologic diagnosis was trichoblastoma. CONCLUSION: Fine needle aspiration cytology can distinguish trichoblastoma from malignant diseases of the breast and may be used to diagnose the lesion in conjunction with clinical findings.
Assuntos
Neoplasias de Anexos e de Apêndices Cutâneos/patologia , Neoplasias Cutâneas/patologia , Idoso , Biópsia por Agulha , Neoplasias da Mama/química , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/patologia , Carcinoma Basocelular/química , Carcinoma Basocelular/diagnóstico , Carcinoma Basocelular/patologia , Diagnóstico Diferencial , Feminino , Humanos , Imuno-Histoquímica , Queratinas/análise , Queratinas/imunologia , Neoplasias de Anexos e de Apêndices Cutâneos/química , Neoplasias de Anexos e de Apêndices Cutâneos/diagnóstico , Neoplasias Cutâneas/química , Neoplasias Cutâneas/diagnósticoRESUMO
The nutritional value of N-acetyl-L-tryptophan (NALT) given intravenously was examined in unrestrained adult rats. They were given solutions of 4% Vuj pattern amino acids, 24.5% glucose, vitamins and electrolytes intravenously for 10 days. They were divided into three groups; one group was then injected intravenously with complete amino acid solution (T group), one with tryptophan-free amino acid solution (TF group) and one group with complete amino acid solution but with NALT instead of tryptophan (NALT). The biological value of the NALT group was 49.6; this value corresponded to 90% of that of the T group. Excretion of injected amino acids totalled about 2.0 to 3.0% in this experiment. The effects of NALT on carcass composition, certain blood constituents and tryptophan pyrrolase activity in the liver were also examined.
Assuntos
Triptofano/análogos & derivados , Tecido Adiposo/metabolismo , Aminoácidos/urina , Animais , Injeções Intravenosas , Masculino , Nitrogênio/metabolismo , Ratos , Triptofano/administração & dosagem , Triptofano/metabolismo , Triptofano Oxigenase/biossínteseRESUMO
The sperm count required were investigated to obtain a conception rate of 80% by unilateral intrauterine insemination (UIUI) of fresh semen in cats. The conception rates obtained by insemination before and after ovulation were also examined. Thirty-six female cats aged 1-7 years were used in the experiments, and the number of experimental cases was 44. Seven male cats aged 2-12 years from which semen could be collected by the artificial vagina method were used. In artificial insemination, 100 iu x 2 or 250 iu of hCG was administered on days 2-4 of estrus, and sperm were introduced into the uterine horn with a greater number of ovulations (or mature follicles) 15, 20 and 30 hr after hCG administration by laparotomy. The inseminated sperm counts were 2 x 10(6) (Exp. 1). 4 x 10(6) (Exp. 2), and 8 x 10(6) (Exp. 3). As a result, ovulation was induced in 42 of 44 cases (induction rate: 95.5%) regardless of the dosage of hCG. Conception was obtained by UIUI in two of 16 animals (conception rate: 12.5%) in the Exp. 1, five of 16 animals (31.3%) in Exp. 2, and eight of 10 animals (80.0%) in Exp. 3. Regarding the relationship between the ovulation state at insemination and conception, the conception rate obtained by insemination before ovulation was clearly higher than that obtained by insemination after ovulation (p<0.05). Regarding the number of kits compared to the number of ovulations on the inseminated side, the percentages of cases in which the number of kits exceeded the number of ovulations on the inseminated side were similar in all groups inseminated with a different number of sperm. It is therefore necessary to investigate conception rates obtained by bilateral insemination to increase the fertility rate. Based on the above findings, it was shown that the sperm count required for fertilization by UIUI is 8 x 10(6).
Assuntos
Gatos/fisiologia , Fertilização/fisiologia , Inseminação Artificial/veterinária , Contagem de Espermatozoides/veterinária , Animais , Gonadotropina Coriônica/administração & dosagem , Feminino , Inseminação Artificial/métodos , Tamanho da Ninhada de Vivíparos , Masculino , Folículo Ovariano/fisiologia , Indução da Ovulação/veterinária , Gravidez , Sêmen/fisiologia , Ultrassonografia , Útero/diagnóstico por imagem , Útero/fisiologiaRESUMO
Frozen feline semen was prepared using two types of extenders, egg yolk Tris-fructose citric acid (EYT-FC) and egg yolk sodium citrate solution (EYC), and the semen qualities after thawing and the conception rates obtained by unilateral intrauterine horn insemination (UIUI) were investigated. Cats used in the experiment were six males and 11 females aged 2-12 years (the number of experimental cases was 17). For preparation of frozen semen, semen collected by the artificial vagina method was adjusted to I x 10(8) sperm/m/ and 7% glycerol, put in 250 microl straws, and then frozen using a cell freezer. The mean sperm motility after thawing was 30.0+/-9.7 (SE) % in the semen prepared with EYT-FC and 30.0+/-3.3% in the semen prepared with EYC. Four of seven animals were fertilized by UIUI using two straws in both extenders, and the conception rate was 57.1%. The mean ratios of number of kits to the number of ovulations in the inseminated side were 61.1+/-24.5% and 30.5+/-3.4% for EYT-FC and EYC, respectively, showing that the ratio tended to be higher in the semen prepared with EYT-FC. The above findings, comparing the two extenders for preparation of frozen feline semen, showed that EYT-FC is slightly superior to EYC. To increase conception and fertility rates, it may be important to increase the sperm count for insemination and to inseminate both uterine horns.
Assuntos
Gatos/fisiologia , Criopreservação/veterinária , Fertilização/fisiologia , Inseminação Artificial/veterinária , Preservação do Sêmen/veterinária , Animais , Gonadotropina Coriônica/administração & dosagem , Criopreservação/métodos , Feminino , Inseminação Artificial/métodos , Tamanho da Ninhada de Vivíparos , Masculino , Folículo Ovariano/fisiologia , Indução da Ovulação/veterinária , Gravidez , Sêmen/fisiologia , Preservação do Sêmen/métodos , Motilidade dos Espermatozoides , Ultrassonografia , Útero/diagnóstico por imagem , Útero/fisiologiaRESUMO
A personal computer based system was constructed to assess the use of various forms of information (multimedia) in patient record keeping. A patient's file with his records kept in a multimedia fashion was made by using the system. We describe the hardware and software construction of the system together with the results and the memory requirements of each type of media. Potential usage of the system in the future is discussed especially in connection with the Picture Archiving and Communications Systems (PACS).
Assuntos
Sistemas Computadorizados de Registros Médicos , Microcomputadores , Sistemas de Informação em Radiologia , Fotografação , Software , Gravação em Fita , Gravação de VideoteipeRESUMO
In this study we analyzed the changing behavior patterns of aged persons seeking medical care at hospitals and clinics over a recent ten-year period. The results showed a rapid increase in the number of aged outpatients receiving care at hospitals since 1977. In contrast, the number of aged outpatients receiving care at clinics since 1977 fluctuated with an overall downward trend, indicating outpatients' preference to seek treatments in hospital as opposed to clinics. The following are expected to affect future outpatient care rate differences between clinics and hospitals: differences in care rates for new outpatients, and differences between the sexes and among the types of illnesses treated. Having an especially large effect are: hypertension and heart diseases, diseases of the circulatory system also known as "adult diseases"; diseases of the digestive system, and diseases of the respiratory system.
Assuntos
Instituições de Assistência Ambulatorial/estatística & dados numéricos , Necessidades e Demandas de Serviços de Saúde/tendências , Hospitais/estatística & dados numéricos , Idoso , Idoso de 80 Anos ou mais , Doença Crônica/classificação , Doença Crônica/epidemiologia , Feminino , Humanos , Japão/epidemiologia , Masculino , Médicos/provisão & distribuição , Fatores Sexuais , Ferimentos e Lesões/classificação , Ferimentos e Lesões/epidemiologiaRESUMO
Each local government conducts health examinations based on the Health and Medical Law for the Aged. However, since some residents are able to take health examinations at their own work places, for example, and the local government are allowed to exclude such people from taking the law-mandated health examination, it is difficult to obtain an accurate picture of the examination rate in each area. We investigated the actual participation status for health examination services of all of the 6,080 persons 20 years and over in age in Sakuragawa-mura, Ibaraki Prefecture. A comparative investigation was made on 3,655 non-bedridden/non-hospitalized persons of 40 years and over to ascertain the reliability of responses to questions about participation in lung cancer and gastric cancer examination services given by the village. The rate of valid responses was extremely low in those who had not participated in health examinations (male 54%, female 55% for the lung cancer, and male 53%, female 56% for the gastric cancer). These discrepancies are assumed to be the result of confusing the current health examinations with: (1) the health examination given in the previous year, (2) other kinds of health examinations, or (3) the health examination given in the work place or the like. A comparative investigation through logistic regression analysis, between the responses to the questions in this investigation and the actual health examination participation records, for persons who had not yet taken either lung cancer examinations or gastric cancer examinations (908 males and 938 females for the former, and 1,038 males and 1,187 females for the latter). Results showed that the influence of (1) and (2) were more or less detected in every kind of cancer examination, and the influence of (1) on the gastric cancer examination was particularly clear. No definite result was obtained about (3), because the actual record of the health examination service at the work place, etc. was unavailable. The results of this study suggests the necessity of a careful examination of methods when conducting a comprehensive service investigation for the health examinations.
Assuntos
Exame Físico/estatística & dados numéricos , Feminino , Humanos , Japão , Masculino , Programas de Rastreamento/estatística & dados numéricos , Análise de Regressão , Inquéritos e QuestionáriosRESUMO
Ours is a general hospital administered directly by the government. There are 585 beds and some 1,200 outpatients on average per day. Visits by home nurses began in September of 1990, and beginning in April of 1994 a home nursing department has been in place at the Community Medical Center. There are 5 persons in charge of the visits. Home visits are paid to 40 patients at present on an ongoing basis. In recent years, malignant diseases have been increasing, and there has been much discussion devoted to the quality of life (QOL) of the patients. In 1994, 15 of the 60 patients visited had malignant diseases. Presently, a 63-year-old female patient with terminal kidney cancer is receiving HPN. Thanks to home nursing visits, she has been able to be discharged. This patient had been receiving home nursing visits from December 21, 1993 until June 20 of 1994 at the rate of 2 visits per month. The person in charge gave guidance for HPN management, and the patient began to regain her pre-hospitalization life and a sense of her role in her family and society. As a result, though she had not actively fought the disease while in the hospital, she gained confidence with the HPN, and with her husband's help, she became a Culture School teacher, resumed her role in society and found her life meaningful. Since there were limits to her husband's ability to care for her, she agreed to enter the hospital again, but was able to continue meaningful life at home for 6 months with assistance from a caregiver, and thus achieved a high QOL.
Assuntos
Serviços de Assistência Domiciliar , Neoplasias Renais/terapia , Nutrição Parenteral no Domicílio , Qualidade de Vida , Cuidadores , Feminino , Humanos , Neoplasias Renais/psicologia , Pessoa de Meia-Idade , Neoplasias Gástricas/psicologia , Neoplasias Gástricas/terapiaRESUMO
It is well known that IVM oocytes show a decreased potential for fertility and development compared with in vivo-matured oocytes. In this study, we added reduced glutathione (GSH) to the fertilization medium during IVF to investigate its effect on the fertility and early embryo development of IVM oocytes. The fertilization rate for IVM oocytes and fresh sperm increased with the addition of GSH (0, 1.0, and 2.0 mM: 51%, 76%, and 70%). Moreover, the addition of GSH to the fertilization medium also improved the developmental potential compared with the control sample (0 mM). In addition, we performed IVF using IVM oocytes and frozen/thawed sperm that had been cryopreserved in a mouse bank. Results indicated a marked increase in the fertilization rate when 1.0 mM GSH was added to the fertilization medium compared with when no GSM was used (0.0 mM GSH: 2% (3/195); 1.0 mM GSH: 33% (156/468)). Furthermore, the fertilization rate improved dramatically via zona drilling using laser equipment (52%: 267/516), whereas normal offspring were obtainsed after transferring embryos created via IVF using IVM oocytes and frozen/thawed sperm. This is the first report in which offspring have been obtained via IVF using IVM oocytes and frozen/thawed sperm.