RESUMO
A diagnostic preparation for the indirect hemagglutination test (IHA) was obtained by sensitization of fixed tannin-treated sheep erythrocytes with IgG-fractions from human herpes zoster convalescent sera. A high specificity of the preparation was established in comparative experiments. It could detect the varicella-herpes-zoster virus antigen in 100% of specimens from patients. The IHA test with this erythrocyte diagnostic preparation is simple, economic, gives the results within 1-1 1/2 hours after the specimens arrive in the laboratory. A great advantage of this test ipesviruses.
Assuntos
Varicela/diagnóstico , Herpes Zoster/diagnóstico , Varicela/imunologia , Eritrócitos/imunologia , Testes de Hemaglutinação , Herpes Zoster/imunologia , Herpesvirus Humano 3/imunologia , HumanosRESUMO
The authors elaborated a method of obtaining stable erythrocytic diagnostic agent intended for titration of immunoglobulins A, M and G in the human blood serum by means of the indirect hemagglutination inhibition test (IHI). The suggested microdroplet method of conducting the IHI test permits to reveal (X +/- m): IgG--0.29 +/- 0.15 microng/ml, IgM--1.56 +/- 0.2 microng/ml, and IgA--0.16 +/- 0.07 microng/ml. The test is highly specific, simple and can be used in practical serological laboratories. The result is obtained in 2 1/2--3 hours.