RESUMO
Nematodes of the genus Trichinella are important zoonotic parasites present throughout Romania. This study aimed to assess the status of Trichinella species in wild animals in Romania over the past 30 years. A literature review of original studies concerning the only two species (out of the four in Europe) of Trichinella (T. spiralis and T. britovi) confirmed in wildlife from Romania was conducted and corroborated with the results of our original research concerning the topic. This review article has shown that, in Romania, European minks were infected with T. spiralis, while wolves, European wild cats, Eurasian lynx, golden jackals, stone marten, and European badgers were infected with T. britovi, respectively. Both Trichinella species have been identified in foxes, bears, wild boars, and ermines, but mixed infections have been found only in European polecats. Trichinella infection is still significantly present in Romania, infecting several wild omnivorous and carnivorous species in an equal manner, with different prevalence rates over the years. Regarding the spatial distribution of T. spiralis and T. britovi in Romania, both species can be found all over the country, but in wild animals, T. britovi is the most prevalent.
RESUMO
Nematodes of the genus Trichinella are among the most widespread parasites of domestic and wild omnivores and predatory animals. The present study aimed to evaluate the antiparasitic effect of Lactobacillus casei ATCC 393 (original) and L. paracasei CNCM in CD-1 mice experimentally infected with Trichinella britovi. Four groups of 20 mice (10 females and 10 males/group) were used, with two control (C) groups and two experimental (E) groups, in which each animal received a daily oral dose of 100 µL of 105 CFU/mL probiotics in Ringer's solution. On day 7, all mice (except the negative control group) were infected orally with Trichinella (100 larvae/animal) as well as the two probiotics. On day 9 post-infection (p.i.), 10 mice/group were euthanized, and the presence of adult parasites in the intestinal content and wall was tested. On day 32 p.i., 10 mice/group were euthanized, then trichinoscopy and artificial digestion were performed to assess the muscle infection with T. britovi. On day 9 p.i., the experimental group pretreated with L. casei ATCC 393 (6.3 ± 3.03) showed a significantly lower number of adult parasites in the intestinal wall compared with the positive control group (24.6 ± 4.78). Additionally, a significantly lower adult parasite count in the intestinal wall was registered in female mice pretreated with L. paracasei CNCM (7.4 ± 4.71) compared to female mice from the positive control (29.0 ± 5.17). No statistically relevant results were obtained concerning the male mice or the data obtained at 32 days p.i., irrespective of mice gender.