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1.
Cell ; 163(2): 340-53, 2015 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-26451484

RESUMO

Stem cells reside in specialized microenvironments known as niches. During Drosophila development, glial cells provide a niche that sustains the proliferation of neural stem cells (neuroblasts) during starvation. We now find that the glial cell niche also preserves neuroblast proliferation under conditions of hypoxia and oxidative stress. Lipid droplets that form in niche glia during oxidative stress limit the levels of reactive oxygen species (ROS) and inhibit the oxidation of polyunsaturated fatty acids (PUFAs). These droplets protect glia and also neuroblasts from peroxidation chain reactions that can damage many types of macromolecules. The underlying antioxidant mechanism involves diverting PUFAs, including diet-derived linoleic acid, away from membranes to the core of lipid droplets, where they are less vulnerable to peroxidation. This study reveals an antioxidant role for lipid droplets that could be relevant in many different biological contexts.


Assuntos
Drosophila/citologia , Drosophila/metabolismo , Gotículas Lipídicas/metabolismo , Nicho de Células-Tronco/efeitos dos fármacos , Animais , Antioxidantes/farmacologia , Proliferação de Células , Drosophila/crescimento & desenvolvimento , Ácidos Graxos Insaturados/farmacologia , Larva/citologia , Larva/crescimento & desenvolvimento , Larva/metabolismo , Neuroglia/metabolismo , Estresse Oxidativo , Oxigênio/metabolismo , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos
2.
Cell ; 146(3): 435-47, 2011 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-21816278

RESUMO

Developing animals survive periods of starvation by protecting the growth of critical organs at the expense of other tissues. Here, we use Drosophila to explore the as yet unknown mechanisms regulating this privileged tissue growth. As in mammals, we observe in Drosophila that the CNS is more highly spared than other tissues during nutrient restriction (NR). We demonstrate that anaplastic lymphoma kinase (Alk) efficiently protects neural progenitor (neuroblast) growth against reductions in amino acids and insulin-like peptides during NR via two mechanisms. First, Alk suppresses the growth requirement for amino acid sensing via Slimfast/Rheb/TOR complex 1. And second, Alk, rather than insulin-like receptor, primarily activates PI3-kinase. Alk maintains PI3-kinase signaling during NR as its ligand, Jelly belly (Jeb), is constitutively expressed from a glial cell niche surrounding neuroblasts. Together, these findings identify a brain-sparing mechanism that shares some regulatory features with the starvation-resistant growth programs of mammalian tumors.


Assuntos
Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Quinase do Linfoma Anaplásico , Animais , Encéfalo/crescimento & desenvolvimento , Encéfalo/metabolismo , Sistema Nervoso Central/crescimento & desenvolvimento , Sistema Nervoso Central/metabolismo , Privação de Alimentos , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Larva/crescimento & desenvolvimento , Larva/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Poliploidia
3.
PLoS Biol ; 19(5): e3001230, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33945525

RESUMO

Obesity-related renal lipotoxicity and chronic kidney disease (CKD) are prevalent pathologies with complex aetiologies. One hallmark of renal lipotoxicity is the ectopic accumulation of lipid droplets in kidney podocytes and in proximal tubule cells. Renal lipid droplets are observed in human CKD patients and in high-fat diet (HFD) rodent models, but their precise role remains unclear. Here, we establish a HFD model in Drosophila that recapitulates renal lipid droplets and several other aspects of mammalian CKD. Cell type-specific genetic manipulations show that lipid can overflow from adipose tissue and is taken up by renal cells called nephrocytes. A HFD drives nephrocyte lipid uptake via the multiligand receptor Cubilin (Cubn), leading to the ectopic accumulation of lipid droplets. These nephrocyte lipid droplets correlate with endoplasmic reticulum (ER) and mitochondrial deficits, as well as with impaired macromolecular endocytosis, a key conserved function of renal cells. Nephrocyte knockdown of diglyceride acyltransferase 1 (DGAT1), overexpression of adipose triglyceride lipase (ATGL), and epistasis tests together reveal that fatty acid flux through the lipid droplet triglyceride compartment protects the ER, mitochondria, and endocytosis of renal cells. Strikingly, boosting nephrocyte expression of the lipid droplet resident enzyme ATGL is sufficient to rescue HFD-induced defects in renal endocytosis. Moreover, endocytic rescue requires a conserved mitochondrial regulator, peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC1α). This study demonstrates that lipid droplet lipolysis counteracts the harmful effects of a HFD via a mitochondrial pathway that protects renal endocytosis. It also provides a genetic strategy for determining whether lipid droplets in different biological contexts function primarily to release beneficial or to sequester toxic lipids.


Assuntos
Lipase/metabolismo , Gotículas Lipídicas/metabolismo , Insuficiência Renal Crônica/metabolismo , Tecido Adiposo/metabolismo , Animais , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Endocitose/fisiologia , Células Epiteliais/metabolismo , Ácidos Graxos/metabolismo , Humanos , Rim/patologia , Lipase/fisiologia , Gotículas Lipídicas/fisiologia , Metabolismo dos Lipídeos/fisiologia , Lipídeos/fisiologia , Mitocôndrias/metabolismo , Obesidade/complicações , Insuficiência Renal Crônica/fisiopatologia , Triglicerídeos/metabolismo
4.
J Proteome Res ; 18(4): 1503-1512, 2019 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-30757904

RESUMO

The measurement of absolute metabolite concentrations in small samples remains a significant analytical challenge. This is particularly the case when the sample volume is only a few microliters or less and cannot be determined accurately via direct measurement. We previously developed volume determination with two standards (VDTS) as a method to address this challenge for biofluids. As a proof-of-principle, we applied VDTS to NMR spectra of polar metabolites in the hemolymph (blood) of the tiny yet powerful genetic model Drosophila melanogaster. This showed that VDTS calculation of absolute metabolite concentrations in fed versus starved Drosophila larvae is more accurate than methods utilizing normalization to total spectral signal. Here, we introduce paired VDTS (pVDTS), an improved VDTS method for biofluids and solid tissues that implements the statistical power of paired control and experimental replicates. pVDTS utilizes new equations that also include a correction for dilution errors introduced by the variable surface wetness of solid samples. We then show that metabolite concentrations in Drosophila larvae are more precisely determined and logically consistent using pVDTS than using the original VDTS method. The refined pVDTS workflow described in this study is applicable to a wide range of different tissues and biofluids.


Assuntos
Metaboloma/fisiologia , Metabolômica/métodos , Aminoácidos/análise , Animais , Carboidratos/análise , Ácidos Carboxílicos/análise , Drosophila melanogaster/química , Drosophila melanogaster/metabolismo , Feminino , Hemolinfa/química , Hemolinfa/metabolismo , Larva/química , Larva/metabolismo , Espectroscopia de Ressonância Magnética , Masculino
5.
Nature ; 481(7382): 516-9, 2012 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-22246326

RESUMO

Mass spectrometry with stable isotope labels has been seminal in discovering the dynamic state of living matter, but is limited to bulk tissues or cells. We developed multi-isotope imaging mass spectrometry (MIMS) that allowed us to view and measure stable isotope incorporation with submicrometre resolution. Here we apply MIMS to diverse organisms, including Drosophila, mice and humans. We test the 'immortal strand hypothesis', which predicts that during asymmetric stem cell division chromosomes containing older template DNA are segregated to the daughter destined to remain a stem cell, thus insuring lifetime genetic stability. After labelling mice with (15)N-thymidine from gestation until post-natal week 8, we find no (15)N label retention by dividing small intestinal crypt cells after a four-week chase. In adult mice administered (15)N-thymidine pulse-chase, we find that proliferating crypt cells dilute the (15)N label, consistent with random strand segregation. We demonstrate the broad utility of MIMS with proof-of-principle studies of lipid turnover in Drosophila and translation to the human haematopoietic system. These studies show that MIMS provides high-resolution quantification of stable isotope labels that cannot be obtained using other techniques and that is broadly applicable to biological and medical research.


Assuntos
Divisão Celular , Espectrometria de Massas/métodos , Células-Tronco/citologia , Células-Tronco/metabolismo , Animais , Animais Recém-Nascidos , DNA/biossíntese , DNA/genética , DNA/metabolismo , Drosophila melanogaster/citologia , Enterócitos/citologia , Fibroblastos/citologia , Humanos , Intestino Delgado/citologia , Marcação por Isótopo , Isótopos , Leucócitos/citologia , Metabolismo dos Lipídeos , Linfopoese , Camundongos , Camundongos Endogâmicos C57BL , Modelos Biológicos , Células-Tronco/patologia , Moldes Genéticos , Timidina/metabolismo
6.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1862(8): 792-796, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28302590

RESUMO

Metabolic pathway flux is a fundamental element of biological activity, which can be quantified using a variety of mass spectrometric techniques to monitor incorporation of stable isotope-labelled substrates into metabolic products. This article contrasts developments in electrospray ionisation mass spectrometry (ESI-MS) for the measurement of lipid metabolism with more established gas chromatography mass spectrometry and isotope ratio mass spectrometry methodologies. ESI-MS combined with diagnostic tandem MS/MS scans permits the sensitive and specific analysis of stable isotope-labelled substrates into intact lipid molecular species without the requirement for lipid hydrolysis and derivatisation. Such dynamic lipidomic methodologies using non-toxic stable isotopes can be readily applied to quantify lipid metabolic fluxes in clinical and metabolic studies in vivo. However, a significant current limitation is the absence of appropriate software to generate kinetic models of substrate incorporation into multiple products in the time domain. Finally, we discuss the future potential of stable isotope-mass spectrometry imaging to quantify the location as well as the extent of lipid synthesis. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein.


Assuntos
Isótopos/química , Metabolismo dos Lipídeos/fisiologia , Lipídeos/química , Animais , Humanos , Marcação por Isótopo/métodos , Redes e Vias Metabólicas/fisiologia , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos
7.
Anal Chem ; 85(24): 12046-54, 2013 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-24251761

RESUMO

The accurate measurement of metabolite concentrations in miniscule biological sample volumes is often desirable, yet it remains challenging. In many cases, the starting analyte volumes are imprecisely known, or not directly measurable, and hence absolute metabolite concentrations are difficult to calculate. Here, we introduce volume determination using two standards (VDTS) as a general quantitative method for the analysis of polar metabolites in submicrolitre samples using (1)H NMR spectroscopy. This approach permits the back calculation of absolute metabolite concentrations from small biological samples of unknown volume. Where small sample volumes are also variable, VDTS can improve multivariate chemometric analysis. In this context, principal component analysis (PCA) yielded more logically consistent and biologically insightful outputs when we used volume-corrected spectra, calculated using VDTS, rather than probabilistic quotient normalization (PQN) of raw spectra. As proof-of-principle, the VDTS-based method and PCA were used to analyze polar metabolites in the hemolymph (blood) extracted from larvae of the very small but widely used genetic model organism Drosophila. This analysis showed that the hemolymph metabolomes of males and females are markedly different when larvae are well fed. However, gender-specific metabolomes tend to converge when larval dietary nutrients are restricted. We discuss the biological implications of these surprising results and compare and contrast them to previous analyses of Drosophila hemolymph and mammalian blood plasma. Together, these findings reveal an interesting and hitherto unknown sexual dimorphism in systemic Drosophila metabolites, clearly warranting further biological investigation. Importantly, the VDTS approach should be adaptable to many different analytical platforms, including mass spectrometry.


Assuntos
Limite de Detecção , Espectroscopia de Ressonância Magnética/normas , Metabolômica/normas , Estatística como Assunto/normas , Animais , Drosophila melanogaster/metabolismo , Feminino , Hemolinfa/metabolismo , Larva/metabolismo , Masculino , Padrões de Referência
8.
Dev Cell ; 11(4): 505-17, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17011490

RESUMO

The sense organs of the vertebrate head comprise structures as varied as the eye, inner ear, and olfactory epithelium. In the early embryo, these assorted structures share a common developmental origin within the preplacodal region and acquire specific characteristics only later. Here we demonstrate a fundamental similarity in placodal precursors: in the chick all are specified as lens prior to acquiring features of specific sensory or neurogenic placodes. Lens specification becomes progressively restricted in the head ectoderm, initially by FGF and subsequently by signals derived from migrating neural crest cells. We show that FGF8 from the anterior neural ridge is both necessary and sufficient to promote olfactory fate in adjacent ectoderm. Our results reveal that placode precursors share a common ground state as lens and progressive restriction allows the full range of placodal derivatives to form.


Assuntos
Indução Embrionária , Fatores de Crescimento de Fibroblastos/metabolismo , Cristalino/embriologia , Órgãos dos Sentidos/embriologia , Animais , Embrião de Galinha , Fatores de Crescimento de Fibroblastos/genética , Imuno-Histoquímica , Hibridização In Situ , Cristalino/citologia , Modelos Biológicos , Técnicas de Cultura de Órgãos
9.
Dev Biol ; 336(2): 327-36, 2009 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-19781543

RESUMO

In vertebrates, cranial placodes form crucial parts of the sensory nervous system in the head. All cranial placodes arise from a common territory, the preplacodal region, and are identified by the expression of Six1/4 and Eya1/2 genes, which control different aspects of sensory development in invertebrates as well as vertebrates. While So and Eya can induce ectopic eyes in Drosophila, the ability of their vertebrate homologues to induce placodes in non-placodal ectoderm has not been explored. Here we show that Six1 and Eya2 are involved in ectodermal patterning and cooperate to induce preplacodal gene expression, while repressing neural plate and neural crest fates. However, they are not sufficient to induce ectopic sensory placodes in future epidermis. Activation of Six1 target genes is required for expression of preplacodal genes, for normal placode morphology and for placode-specific Pax protein expression. These findings suggest that unlike in the fly where the Pax6 homologue Eyeless acts upstream of Six and Eya, the regulatory relationships between these genes are reversed in early vertebrate placode development.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/fisiologia , Animais , Embrião de Galinha , Proteínas de Homeodomínio/genética , Humanos , Imuno-Histoquímica , Hibridização In Situ , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/fisiologia , Proteínas Nucleares/genética , Proteínas Nucleares/fisiologia , Fator de Transcrição PAX2/genética , Proteínas Tirosina Fosfatases/genética , Proteínas Tirosina Fosfatases/fisiologia
10.
Nat Commun ; 2: 265, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21468017

RESUMO

In vertebrates, the lens and retina arise from different embryonic tissues raising the question of how they are aligned to form a functional eye. Neural crest cells are crucial for this process: in their absence, ectopic lenses develop far from the retina. Here we show, using the chick as a model system, that neural crest-derived transforming growth factor-ßs activate both Smad3 and canonical Wnt signalling in the adjacent ectoderm to position the lens next to the retina. They do so by controlling Pax6 activity: although Smad3 may inhibit Pax6 protein function, its sustained downregulation requires transcriptional repression by Wnt-initiated ß-catenin. We propose that the same neural crest-dependent signalling mechanism is used repeatedly to integrate different components of the eye and suggest a general role for the neural crest in coordinating central and peripheral parts of the sensory nervous system.


Assuntos
Olho/metabolismo , Crista Neural/metabolismo , Transdução de Sinais , Proteínas da Superfamília de TGF-beta/metabolismo , Proteína Wnt2/metabolismo , Animais , Embrião de Galinha , Galinhas , Olho/citologia , Olho/embriologia , Cristalino/embriologia , Cristalino/metabolismo , Modelos Biológicos , Crista Neural/citologia , Crista Neural/embriologia , Proteína Smad3/genética , Proteína Smad3/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Proteína Wnt2/genética
11.
Cell Metab ; 13(1): 92-104, 2011 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-21195352

RESUMO

The gastrointestinal tract is emerging as a key regulator of appetite and metabolism, but daunting neuroanatomical complexity has hampered identification of the relevant signals. Invertebrate models could provide a simple and genetically amenable alternative, but their autonomic nervous system and its visceral functions remain largely unexplored. Here we develop a quantitative method based on defecation behavior to uncover a central role for the Drosophila intestine in the regulation of nutrient intake, fluid, and ion balance. We then identify a key homeostatic role for autonomic neurons and hormones, including a brain-gut circuit of insulin-producing neurons modulating appetite, a vasopressin-like system essential for fluid homeostasis, and enteric neurons mediating sex peptide-induced changes in intestinal physiology. These conserved mechanisms of visceral control, analogous to those found in the enteric nervous system and hypothalamic/pituitary axis, enable the study of autonomic control in a model organism that has proved instrumental in understanding sensory and motor systems.


Assuntos
Sistema Nervoso Entérico/fisiologia , Trato Gastrointestinal/inervação , Neurônios Eferentes/fisiologia , Células Receptoras Sensoriais/fisiologia , Equilíbrio Ácido-Base , Animais , Regulação do Apetite/fisiologia , Dieta , Diurese , Drosophila/metabolismo , Drosophila/fisiologia , Feminino , Trato Gastrointestinal/metabolismo , Trato Gastrointestinal/fisiologia , Trânsito Gastrointestinal , Hemostáticos , Masculino , Reprodução , Atrativos Sexuais/fisiologia , Vasopressinas/fisiologia
12.
Curr Top Dev Biol ; 72: 167-204, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16564335

RESUMO

Sensory placodes are unique domains of thickened ectoderm in the vertebrate head that form important parts of the cranial sensory nervous system, contributing to sense organs and cranial ganglia. They generate many different cell types, ranging from simple lens fibers to neurons and sensory cells. Although progress has been made to identify cell interactions and signaling pathways that induce placodes at precise positions along the neural tube, little is known about how their precursors are specified. Here, we review the evidence that placodes arise from a unique territory, the pre-placodal region, distinct from other ectodermal derivatives. We summarize the cellular and molecular mechanisms that confer pre-placode character and differentiate placode precursors from future neural and neural crest cells. We then examine the events that subdivide the pre-placodal region into individual placodes with distinct identity. Finally, we discuss the hypothesis that pre-placodal cells have acquired a state of "placode bias" that is necessary for their progression to mature placodes and how such bias may be established molecularly.


Assuntos
Ectoderma/fisiologia , Cabeça/embriologia , Órgãos dos Sentidos/embriologia , Animais , Humanos
13.
Dev Biol ; 271(2): 403-14, 2004 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-15223343

RESUMO

Cranial placodes are focal regions of columnar epithelium next to the neural tube that contribute to sensory ganglia and organs in the vertebrate head, including the olfactory epithelium and the crystalline lens of the eye. Using focal dye labelling within the presumptive placode domain, we show that lens and nasal precursors arise from a common territory surrounding the anterior neural plate. They then segregate over time and converge to their final positions in discrete placodes by apparently directed movements. Since these events closely parallel the separation of eye and antennal primordia (containing olfactory sensory cells) from a common imaginal disc in Drosophila, we investigated whether the vertebrate homologues of Distalless (Dll) and Eyeless (Ey), which determine antennal and eye identity in the fly, play a role in segregation of lens and nasal precursors in the chick. Dlx5 and Pax6 are initially co-expressed by future lens and olfactory cells. As soon as presumptive lens cells acquire columnar morphology all Dlx family members are down-regulated in the placode, while Pax6 is lost in the olfactory region. Lens precursor cells that express ectopic Dlx5 never acquire lens-specific gene expression and are excluded from the lens placode to cluster in the head ectoderm. These results suggest that the loss of Dlx5 is required for cells to adopt a lens fate and that the balance of Pax6 and Dlx expression regulates cell sorting into appropriate placodal domains.


Assuntos
Linhagem da Célula/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Homeodomínio/metabolismo , Cristalino/embriologia , Crista Neural/embriologia , Mucosa Olfatória/embriologia , Animais , Movimento Celular , Embrião de Galinha , Eletroporação , Proteínas do Olho , Imuno-Histoquímica , Hibridização In Situ , Cristalino/citologia , Morfogênese , Crista Neural/citologia , Mucosa Olfatória/citologia , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados , Proteínas Repressoras , Gravação em Vídeo
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