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In the present study bacterial strains were isolated from the rumen fluids of Bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens Vibrio cholera, Enterococcus faecalis, Enterobacter aerogens, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Moreover it showed above 70% cell surface hydrophobicity, significant low-invasion ability and potential adherence capacity in Caco-2 cells when compared with the control. The proinflammatory cytokines (TNF-α) was greatly reduced in rumen bacteria treatment and ARBS-1 modulate the immune response by activating the IL-4 secretion in parallel to TNF-α suppression. The 16s rRNA gene sequence of the active isolates were identified as Enterococcus hirae (ARBS-1), Pediococcus acidilactici (ARBS-4) and Bacillus licheniformis (ARBS-7). This study revealed the probiotic bactericidal properties of E. hirae obtained from the rumen of B. primigenius with potential antibacterial and anti-inflammatory effects. Future studies with the strains may yield some novel probiotic product for livestock's.
Assuntos
Antibacterianos/farmacologia , Bovinos/microbiologia , Enterococcus/efeitos dos fármacos , Probióticos/farmacologia , Rúmen/microbiologia , AnimaisRESUMO
BACKGROUND: Sexual dimorphism in teeth has been an area of research for forensic anthropologists. The function of root in transmitting the forces of occlusion to the alveolar bone varies as the force in males tends to be larger than in females. This shows the significance of the root length as indicators of sexual dimorphism. AIM: The aim of this study was to determine the presence of sexual dimorphism in the root lengths of permanent teeth and to evaluate if root length could be instrumental in defining sexual dimorphism among an ethnic Tamil population. MATERIALS AND METHODS: Orthopantomograms of 1000 individuals (500 males and 500 females) were utilized, and the measurement of root length of permanent maxillary and mandibular teeth from canine to first molar on all four quadrants using the Digital software SCANORA 5.2.6. was carried out. Statistical analysis including descriptive statistics and independent Student t-test were performed. RESULTS: In this study, the sexual dimorphism in root length is observed in 13, 14, 15, 16, 23, 26, 33, 36, 43, and 46 (mesial), and there is a statistically significant difference between the root measurements of males and females (P < 0.05). Most dimorphic teeth were maxillary canines and mandibular canines. CONCLUSION: The data generated from this study suggest that the root length measurements present with a substantial evidence of sexual dimorphism emphasizing its importance on identifying sex and are therefore useful in determining the biological profile.
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BACKGROUND: Forensic odontology plays a pivotal role in the identification of victims in mass disasters utilizing "preserved dental records" or "ante-mortem records" available with the general dental practitioners. Identification of a deceased individual by comparing antemortem and postmortem records is more reliable and easier as compared to other methods. However, in India, the practice of maintaining dental case record requires additional emphasis. AIM: The aim of the present study is to assess the knowledge, attitude, and practices of dental practitioners in South India regarding awareness and importance of maintaining patient's dental records. SETTINGS AND DESIGN: A descriptive questionnaire study was conducted among the alumni of the dental institution over a period of 3 months. The alumni who passed out from 2000 to 2015 were included in the study. MATERIALS AND METHODS: A structured questionnaire containing 24 questions regarding the knowledge, attitude, and practice of maintaining clinical case records was prepared and validated. Data were collected from 543 dental practitioners from various parts of South India who were the alumni of the dental institution. STATISTICAL ANALYSIS: The data were summarized and analyzed using the statistical software Statistical Package for the Social Sciences. The descriptive analysis was done in percentages, and the results were tabulated. RESULTS: Overall, 58.39% of dentists were found to be maintaining records promptly, and 84.6% dentists have knowledge about forensic odontology, but only 8.4% of dentists have helped the government agencies during mass disasters. CONCLUSION: This study reveals that the need of the hour is to change the attitude and to cultivate interest among practicing dentists regarding dental record maintenance which can tremendously help in the deceased individual identification process.
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Oral squamous cell carcinoma (OSCC) is the most common epithelial malignant neoplasm affecting the oral cavity; early detection is an important criterion for achieving high cure rate. Occasionally, it may be misdiagnosed because of its variable and innocuous clinical appearance. Carcinomas of the gingiva are a unique subset of OSCC, constituting approximately 10% of OSCCs and can mimic a multitude of oral lesions especially those of inflammatory origin with benign features, often leading to delay in the diagnosis and hence delayed treatment. This article reports a rare case of gingival OSCC in a 62-year-old female patient mimicking an inflammatory gingival mass.
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Rhinosporidiosis caused by Rhinosporidium seeberi is a fungal infection, which affects chiefly the mucus membranes of the nose, oropharynx, and nasopharynx, as well as the larynx, skin, eyes, and genital mucosa. Soil and water act as a reservoir for the organism. Here, we present a case of Rhinosporidiosis, which clinically manifested as a facial swelling indicating that fungal infections should also be considered as one of the differential diagnoses whenever facial swellings are encountered.