Differential effects of continuous and intermittent daytime food deprivation periods on metabolism and reproductive performance in diurnal zebra finches.

We investigated whether food availability effects on metabolism and reproduction are the result of the sum effect of daily feeding (food availability) and starvation (food deprivation) periods. Adult zebra finches were paired and subjected to a time-restricted feeding (TRF) regimen consisting of continuous and intermittent daytime food deprivation periods. Birds were given food during the 12-h day for a total of 4-h in the evening (1 *4-h, hour 8-12), or in 2 splits of 2 h each (2 * 2-h) or 4 splits 1 h each (4 * 1-h), with controls on food ad libitum, until they had the first egg clutch. TRF caused significant changes in hepatic expression of metabolism-associated sirt1, egr1, pparα and foxo1 genes despite no difference in the food intake, body mass and blood glucose levels. Importantly, TRF resulted in a significant reduction in plasma testosterone and estradiol levels, delayed nest-building and egg laying, and reduced clutch size. Concurrently, under TRF regimes, we found a significantly lower expression of th and mtr genes linked with motivation and affiliation (but not of dio2, dio3, gnrh1 and gnih genes linked with gonadal maturation) in the hypothalamus, and of star and hook 1 genes in the testes and star, cyp19 and erα genes in the ovary. These results demonstrate the importance of daily food deprivation times on the metabolism and reproduction, and suggest a possible provisioning of energy available from daily feeding for the maintenance of body condition at the expense of reproduction performance in diurnal animals.

Artificial night illumination disrupts sleep, and attenuates mood and learning in diurnal animals: evidence from behavior and gene expression studies in zebra finches.

This study investigated the effects of an illuminated night on sleep, mood, and cognitive performance in non-seasonal diurnal zebra finches that were exposed for 6 weeks to an ecologically relevant dimly lit night (12L:12dLAN; 150 lx: 5 lx) with controls on the dark night (12L:12D; 150 lx: < 0.01 lx). Food and water were provided ad libitum. Under dLAN (dim light at night), birds showed disrupted nocturnal (frequent awakenings) and overall decreased sleep duration. They also exhibited a compromised novel object exploration, a marker of the bird's mood state, and committed more errors, took significantly longer duration to learn with low retrieval performance of the learned task when tested for a color-discrimination (learning) task under the dLAN. Further, compared to controls, there was reduced mRNA expression level of genes involved in the neurogenesis, neural plasticity (bdnf, dcx and egr1) and motivation (th, drd2, taar1 and htr2c; dopamine synthesis and signaling genes) in the brain (hippocampus (HP), nidopallium caudolaterale (NCL), and midbrain) of birds under dLAN. These results show concurrent negative behavioral and molecular neural effects of the dimly illuminated nights, and provide insights into the possible impact on sleep and mental health in diurnal species inhabiting an increasingly urbanized ecosystem.

Molecular changes associated with migratory departure from wintering areas in obligate songbird migrants.

Day length regulates the development of spring migratory and subsequent reproductive phenotypes in avian migrants. This study used molecular approaches, and compared mRNA and proteome-wide expression in captive redheaded buntings that were photostimulated under long-day (LD) conditions for 4 days (early stimulated, LD-eS) or for ∼3 weeks until each bird had shown 4 successive nights of Zugunruhe (stimulated, LD-S); controls were maintained under short days. After ∼3 weeks of LD, photostimulated indices of the migratory preparedness (fattening, weight gain and Zugunruhe) were paralleled with upregulated expression of acc, dgat2 and apoa1 genes in the liver, and of cd36, fabp3 and cpt1 genes in the flight muscle, suggesting enhanced fatty acid (FA) synthesis and transport in the LD-S state. Concurrently, elevated expression of genes involved in the calcium ion signalling and transport (camk1 and atp2a2; camk2a in LD-eS), cellular stress (hspa8 and sod1, not nos2) and metabolic pathways (apoa1 and sirt1), but not of genes associated with migratory behaviour (adcyap1 and vps13a), were found in the mediobasal hypothalamus (MBH). Further, MBH-specific quantitative proteomics revealed that out of 503 annotated proteins, 28 were differentially expressed (LD-eS versus LD-S: 21 up-regulated and 7 down-regulated) and they enriched five physiological pathways that are associated with FA transport and metabolism. These first comprehensive results on gene and protein expression suggest that changes in molecular correlates of FA transport and metabolism may aid the decision for migratory departure from wintering areas in obligate songbird migrants.

Daytime light spectrum affects photoperiodic induction of vernal response in obligate spring migrants.

It is not well understood how the spectral composition (wavelength) of daylight that varies considerably during the day and seasons affects photoperiodic responses in a seasonal species. Here, we investigated the molecular underpinnings of wavelength-dependent photoperiodic induction in migratory redheaded buntings transferred to 13 h long days in neutral (white), 460 nm (blue), 500 nm (green) or 620 nm (red) wavelength that were compared with one another, and to short day controls for indices of the migratory (body fattening and weight gain, and Zugunruhe) and reproductive (testicular maturation) responses. Buntings showed wavelength-dependent photoperiodic response, with delayed Zugunruhe and slower testis maturation under 620 nm red light. Post-mortem comparison of gene expressions further revealed wavelength-dependence of the photoperiodic molecular response. Whereas there were higher retinal expressions of opn2 (rhodopsin) and opn5 (neuropsin) genes in red daylight, and of rhodopsin-like opsin (rh2) gene in green daylight, the hypothalamic opn2 mRNA levels were higher in blue daylight. Similarly, we found in birds under blue daylight an increased hypothalamic expression of genes involved in the photoperiodic induction (thyroid stimulating hormone subunit beta, tshb; eye absent 3, eya3; deiodinase type 2, dio2) and associated neural responses such as the calcium signaling (ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2, atp2a2), dopamine biosynthesis (tyrosine hydroxylase, th) and neurogenesis (brain-derived neurotrophic factor, bdnf). These results demonstrate transcriptional changes in parallel to responses associated with migration and reproduction in buntings, and suggest a role of daylight spectrum in photoperiodic induction of the vernal response in obligate spring avian migrants.

Sleep in unnatural times: illuminated night negatively affects sleep and associated hypothalamic gene expressions in diurnal zebra finches.

We investigated the effects of exposure at ecologically relevant levels of dim light at night (dLAN) on sleep and the 24 h hypothalamic expression pattern of genes involved in the circadian timing (per2, bmal1, reverb-ß, cry1, ror-α, clock) and sleep regulatory pathways (cytokines: tlr4, tnf-α, il-1ß, nos; Ca2+-dependent pathway: camk2, sik3, nr3a; cholinergic receptor, achm3) in diurnal female zebra finches. Birds were exposed to 12 h light (150 lux) coupled with 12 h of absolute darkness or of 5 lux dim light for three weeks. dLAN fragmented the nocturnal sleep in reduced bouts, and caused sleep loss as evidenced by reduced plasma oxalate levels. Under dLAN, the 24 h rhythm of per2, but not bmal1 or reverb-ß, showed a reduced amplitude and altered peak expression time; however, clock, ror-α and cry1 expressions showed an abolition of the 24 h rhythm. Decreased tlr4, il-1ß and nos, and the lack of diurnal difference in achm3 messenger RNA levels suggested an attenuated inhibition of the arousal system (hence, awake state promotion) under dLAN. Similarly, changes in camk2, sik3 and nr3a expressions suggested dLAN-effects on Ca2+-dependent sleep-inducing pathways. These results demonstrate dLAN-induced negative effects on sleep and associated hypothalamic molecular pathways, and provide insights into health risks of illuminated night exposures to diurnal animals.

Involvement of steroid and antioxidant pathways in spleen-mediated immunity in migratory birds.

The molecular underpinnings of the spleen-mediated immune functions during the period of heightened energetic needs in the year are not known in avian migrants. We investigated this, in Palearctic-Indian migratory male redheaded buntings, which exhibited vernal (spring) premigratory / early testicular maturation states under artificial long days. This was evidenced by increased dio2 and decreased dio3 mRNA expression in the hypothalamus, elevated levels of circulating corticosterone and testosterone, and enlarged testes in long-day-photostimulated birds, as compared to unstimulated controls under short days. The concomitant decrease in both mass and volume of the spleen, and increase in the heterophil/ lymphocyte ratio suggested the parallel innate immunity effects in photostimulated buntings. Importantly, we found increased mRNA expression of genes coding for the cytokines (il15 and il34), steroid receptors (nr3c2) and oxidative stress marker enzymes (gpx1 and sod1) in the spleen, suggesting the activation of both immune and antioxidant molecular pathways during the early photostimulated state. However, the splenic expressions of il1ß, il6, tgfß, ar and nos2 genes were not significantly different between long-day stimulated and short-day unstimulated birds. The negative correlation of plasma corticosterone levels with spleen mass further indicated a role of corticosterone in the modulation of the spleen function, probably via nr3c2 gene encoded mineralocorticoid receptors. These results suggest the activation of the spleen-mediated innate immunity in anticipation of the heightened energetic stress state of the photostimulated spring migratory/breeding period in migratory songbirds.

Effects of light intensity on circadian activity behaviour in the Indian weaverbird (Ploceus philippinus).

Circadian (locomotor activity/perching) behaviour of the weaverbird (Ploceus philippinus) under different light intensities was studied. Six groups of birds were subjected to 12L:12D (L = 1000 and 10 lux and D = 0.3 lux) for two weeks, and thereafter released into constant dim illumination (LL(dim) = 0.3 lux). After two weeks of LL(dim), birds were given a 2 h light pulse of 1000 lux at circadian time (CT) 12, 17 and 20, and exposure of LL(dim) was continued for another two weeks and the activity pattern was monitored. As expected, all birds were entrained under 12L:12D showing dense-activity in the group that was placed under light phase of 1000 lux. Under LL(dim) birds exhibited circadian activity rhythms with periods longer or shorter than 24 h. Light pulse at CT 12 caused small delay shift in the activity phase, but a larger delay in phase shift occurred when the pulse was given at CT 17. A pulse at CT 20 caused small advanced phase shift. Thus, photoperiodic weaverbird appears to show circadian system regulated behaviour as seen by activity-rest pattern under programmed light cycles.

Reproductive biology of Indian Silurid catfish Ompok pabda in river Gomti.

Reproductive biology of an Indian Silurid catfish, Ompok pabda were determined in a moderately impacted tropical River Gomti in India. Samples offish were collected monthly between May 2008 to April 2009 in two zones; upstream site at Mishrikh, Sitapur and midstream site at Lucknow. Length at first maturity calculated through maturity curve was 12.9 cm (males) and 13.9 cm (females) in total length. The gonado-somatic index indicated that spawning generally occurred between June - September and sex ratio showed a predominance of females, representing 54.3% of the total sample. The fecundity was found to vary from 2460 to 5986 nos, with mean of 4330 +/- 799 for the fish with total length of 11.5-20.0 cm. The relationship of fecundity with total length and total body weight of the fish was Log F = 0.374 Log L+ 0.37, r2 = 0.999 and Log F = 0.337 Log W-0.40, r2 = 0.966. The well defined traits of O. pabda from the Ganges basin provide basic information which can be further used for species conservation planning.

Key Learnings from the Development and Early Use of Global Guidance on the Integration of COVID-19 Vaccination into Broader Health Systems.

More than 13.5 billion COVID-19 vaccine doses were delivered between 2021 and 2023 through a mix of delivery platforms, with mass vaccination campaigns being the main approach. In 2022, with the continued circulation of SARS-CoV2 and the need for periodic boosters being most likely, countries were required to plan for more sustainable approaches to provide COVID-19 vaccinations. In this context of uncertainty, a global tool for integrating COVID-19 vaccines into immunization programs and as part of broader health systems was published jointly by the WHO and UNICEF to respond to country needs. This paper summarizes the approach to, and lessons learned during, the development of a global guidance document and describes some examples of its early use in low- and middle-income countries (LMICs). The guidance leveraged existing health system frameworks, proposed four steps for planning and implementing the COVID-19 vaccination integration journey, and identified investment areas. The development process maximized robust global stakeholder and country engagement, and the timeframe was aligned with donor funding windows to support countries with the integration of COVID-19 vaccination. The rapid dissemination of the guidance document allowed countries to ascertain their readiness for integrating COVID-19 vaccination and inform the development of national plans and funding applications. While progress has been made in specific areas (e.g., optimizing cold chain and logistics leveraging COVID-19 vaccination), in the context of decreasing demand for COVID-19 vaccines, reaching adult COVID-19 vaccine high-priority-use groups and engaging and coordinating with other health programs (beyond immunization) remain challenges, particularly in LMICs. We share the learning that despite the uncertainties of a pandemic, guidance documents can be developed and used within a short timeframe. Working in partnership with stakeholders within and beyond immunization towards a common objective is powerful and can allow progress to be made in terms of integrating health services and better preparing for future pandemics.

Night melatonin levels affect cognition in diurnal animals: Molecular insights from a corvid exposed to an illuminated night environment.

This study investigated the role of nocturnal melatonin secretion in the cognitive performance of diurnal animals. An initial experiment measured the cognitive performance in Indian house crows treated for 11 days with 12 h light at 1.426 W/m2 (∼150 lux) coupled with 12 h of 0.058 W/m2 (∼6-lux) dim light at night (dLAN) or with absolute darkness (0 lux dark night, LD). dLAN treatment significantly decreased midnight melatonin levels and negatively impacted cognitive performance. Subsequently, the role of exogenous melatonin (50 µg; administered intraperitoneally half an hour before the night began) was assessed on the regulation of cognitive performance in two separate experimental cohorts of crows kept under dLAN; LD controls received vehicle. Exogenous melatonin restored its mid-night levels under dLAN at par with those under LD controls, and improved the cognitive performance, as measured in the innovative problem-solving, and spatial and pattern learning-memory efficiency tests in dLAN-treated crows. There were concurrent molecular changes in the cognition-associated brain areas, namely the hippocampus, nidopallium caudolaterale and midbrain. In particular, the expression levels of genes involved in neurogenesis and synaptic plasticity (bdnf, dcx, egr1, creb), and dopamine synthesis and signalling (th, drd1, drd2, darpp32, taar1) were restored to LD control levels in crows treated with illuminated nights and received melatonin. These results demonstrate that the maintenance of nocturnal melatonin levels is crucial for an optimal higher-order brain function in diurnal animals in the face of an environmental threat, such as light pollution.

Prolonged Lockdown due to COVID-19 Alters Sleep-Wake Timings and Negatively Affects Self-esteem, Personality, Depression and Anxiety in College-Going Indian Students.

Purpose: COVID-19 forced the shutdown of colleges and socialization around the world including India and prolonged lockdown could have a significant impact on sleep, mood, emotion and anxiety in students. Here, we designed a survey to assess the effect of lockdown on sleep-wake, self-esteem, depression and anxiety via the survey on 321 Indian college-going students. Methods: We assessed the effect of lockdown on sleep-wake (wake up, get up, bed and sleep timing, total time in bed and sleep duration) and self-esteem and depression on students via various questions. Results: We found that students delayed sleep timing by 1 h in lockdown than pre-lockdown days. Specifically, urban male populations were late sleeper than any other group. However, total sleep duration did not differ between groups. In self-esteem questions, most students agreed that they were of no good, felt nervous, displeased, and frustrated about performance in lockdown. Depression and anxiety questionnaire gave more significant results related to mood and mental health. More than 50% of students agreed that they faced a lack of concentration, had been feeling irritated and angry, felt fatigued and tired, and everything had been a failure during lockdown days. Conclusion: Overall, the survey suggests that lack of social life and prolonged lockdown affect the sleep-wake cycle, self-esteem, anxiety and depression of Indian students. Supplementary Information: The online version contains supplementary material available at 10.1007/s41782-022-00200-9.

Magnetic Compass Orientation in a Palaearctic-Indian Night Migrant, the Red-Headed Bunting.

Red-headed Buntings (Emberiza bruniceps) perform long-distance migrations within their southerly overwintering grounds and breeding areas in the northern hemisphere. Long-distance migration demands essential orientation mechanisms. The earth's magnetic field, celestial cues, and memorization of geographical cues en route provide birds with compass knowledge during migration. Birds were tested during spring migration for orientation under natural clear skies, simulated overcast skies at natural day length and temperature, simulated overcast at 22 °C and 38 °C temperatures, and in the deflected (-120°) magnetic field. Under clear skies, the Red-headed Buntings were oriented NNW (north-northwest); simulated overcast testing resulted in a northerly mean direction at local temperatures as well as at 22 °C and 38 °C. The Buntings reacted strongly in favor of the rotated magnetic field under the simulated overcast sky, demonstrating the use of a magnetic compass for migrating in a specific direction.

Developmental effects of daily food availability times on song behaviour and neuronal plasticity of song-control system in male zebra finches.

Food availability is a major ecological factor and affects body condition and sexual traits. Here, we investigated whether males' song behaviour, a trait for female mate choice, was sensitive to the food availability period and its timing in songbirds. We manipulated daily food availability to 4 h in the morning or evening, with controls on food ad libitum, and assessed its effects on song behaviour and forebrain song control system in male zebra finches that were held as adult (parent) or offspring (since birth) at 24 ± 2 °C under 12 h daily photoperiod. Food restriction significantly affected both temporal and spectral features of daily song in offspring, not the parent. In offspring, we found reduced mesor (mean 24-h levels), attenuated amplitude (daily maxima relative to mesor) and altered acrophase (estimated time of daily maxima) of 24-h rhythm, and reduced motif length (in morning-fed), per motif unique syllables and an enhanced song pitch (in evening-fed). There was also a positive correlation of motif length with cheek patch and plasma testosterone levels, and of per motif syllables with cheek patch and daily activity levels in offspring. Among main song controlling forebrain nuclei, LMAN and HVC were reduced in size, and Area X and HVC showed decreased neuronal recruitment in offspring on food restrictions. These results demonstrate the importance of daily food availability and its timing in determining males' sexual signals, and support growing evidence that among vertebrates well-fed males contain reproductive traits that females use for its mate choice.

Changes in brain peptides associated with reproduction and energy homeostasis: Putative roles of gonadotrophin-releasing hormone-II and tyrosine hydroxylase in determining reproductive performance in response to daily food availability times in diurnal zebra finches.

Previous studies have demonstrated 'quality-quantity' trade-offs with daily food availability times in zebra finches. Compared with food access ad lib., zebra finch pairs with restricted food access for 4 hours in the morning produced poor quality offspring, whereas those with the same food access in the evening produced fewer but better quality offspring. The present study investigated whether food-time-dependent differential effects on reproductive performance involved brain peptides associated with reproduction and energy homeostasis in zebra finches. We measured peptide/protein expression of gonadotrophin-releasing hormone (GnRH)-I, GnRH-II, gonadotrophin-inhibitory hormone (GnIH), tyrosine hydroxylase (TH), neuropeptide Y (NPY), cocaine- and amphetamine regulated transcript (CART) and ZENK (a neuronal activation marker) by immunohistochemistry and mRNA expression of genes coding for the type 2 (DIO2) and type 3 (DIO3) deiodinase by a quantitative polymerase chain reaction in male and female zebra finches that were paired and kept under a 12:12 hour light/dark photocycle at 24 ± 2°C temperature for > 12 months with access to food ad lib., or for only 4 hours in the morning or evening. In both sexes, GnRH-I, DIO2 and DIO3 expression did not differ significantly between the three feeding conditions, although levels showed an overall food effect. However, in males, GnIH expression was significantly higher in evening-fed birds compared to ad lib. fed birds. Interestingly, GnRH-II and TH levels were significantly lower in restricted feeding compared to the ad lib. group and, importantly, GnRH-II and TH-immunoreactivity levels were negatively and positively correlated with egg laying latency and reproductive success (offspring/brood/pair), respectively. At the same time, we found no effect on the hypothalamic expression of orexigenic (NPY) and anorexigenic (CART) peptides, or ZENK protein (ie, the neuronal activity marker). These results suggest the involvement of reproductive neuropeptides, with putative roles for GnRH-II and TH, in the food-time-dependent effect on reproductive performance, albeit with subtle sex differences, in diurnal zebra finches, which possess the ability to reproduce year-round, in a manner similar to other continuously breeding vertebrates.

Developmental effects of constant light on circadian behaviour and gene expressions in zebra finches: Insights into mechanisms of metabolic adaptation to aperiodic environment in diurnal animals.

A most crucial feature of biological adaptation is the maintenance of a close temporal relationship of behaviour and physiology with prevailing 24-h light-dark environment, which is rapidly changing with increasing nighttime illumination. This study investigated developmental effects of the loss of night on circadian behaviour, metabolism and gene expressions in diurnal zebra finches born and raised under LL, with controls on 12L:12D. Birds under LD were entrained, and showed normal body mass and a significant 24-h rhythm in both activity-rest pattern and mRNA expression of candidate genes that we measured. But, under LL, birds gained weight and accumulated lipid in the liver. Intriguingly, at the end of the experiment, the majority (4/5th) of birds under LL were rhythmic in activity despite arrhythmic expression in the hypothalamus of c-Fos (neuronal activity), Rhodopsin and Mel1-a genes (light perception), and clock genes (Bmal1, Per2 and Rev-erb ß). In peripheral tissues, LL induced variable clock gene expressions. Whereas 24-h mRNA rhythm was abolished for Bmal1 in both liver and gut, it persisted for Per2 and Rev-erb ß in liver, and for Per2 in gut. Further, we found under LL, the loss of 24-h rhythm in hepatic expression of Fasn and Cd36/Fat (biosynthesis and its uptake), and gut expression of Sglt1, Glut5, Cd36 and Pept1 (nutrient absorption) genes. As compared to LD, baseline mRNA levels of Fasn and Cd36 genes were attenuated under LL. Among major transporter genes, Sglt1 (glucose) and Cd36 (fat) genes were arrhythmic, while Glut5 (glucose) and Pept1 (protein) genes were rhythmic but with phase differences under LL, compared to LD. These results demonstrate dissociation of circadian behaviour from clock gene rhythms, and provide molecular insights into possible mechanisms at different levels (behaviour and physiology) that diurnal animals might employ in order to adapt to an emerging overly illuminated-night urban environment.

Temperature affects liver and muscle metabolism in photostimulated migratory redheaded buntings (Emberiza bruniceps).

We investigated the transcriptional effects of temperature on metabolism in the liver and flight muscle prior to the onset of spring migration in redheaded buntings. We measured changes in body mass, cellular lipid accumulation, and transcription of metabolic genes in the liver and flight muscle, during a week of exposure to 8-h short photoperiod (SP; 8L: 16D) and 13-h-long photoperiod (LP; 13L: 11D) at 22 °C to ascertain the responsiveness to LP, and during 2.5 weeks of LP at 22 and 38 °C to examine the effect of temperature on LP-induced metabolic response. Particularly, we measured mRNA expression of genes involved in the fatty acids and triglycerides biosynthesis (srebf1, elovl6, scd, fads2, and dgat2), glucose and lipid metabolism (sirt1, foxo1, ppara, pparg, hdac3, and hmgcr) in the liver, and fatty acid transport (cd36 and fabp3) and metabolism (myod1) in the flight muscle. Birds gained weight and had elevated lipid accumulation at 22 °C but not at 38 °C in the liver and muscle tissues. We also found significant differences in gene expression patterns during 2.5 weeks of LP exposure. For instance, as compared to week 1, there were decreased elovl6, dgat2, and srebf1 and increased foxo1 mRNA levels in the liver, and decreased cd36 and fabp3 mRNA levels in the muscle at 38 °C after 2.5 weeks of LP. It is suggested that a drastic change in temperature could alter the molecular metabolism in the liver and skeletal muscle with photoperiod-induced transition in the seasonal state in migratory songbirds.

Bright light at night alters the perception of daylength in Indian weaver bird (Ploceus philippinus).

The brighter nights have posed new challenges to the wild species by affecting their temporal physiology. The present study on Indian weaver bird (Ploceus philippinus) investigated if exposure to bright light at different phases of night affects their clock-mediated daily functions. Birds were placed individually in specially designed activity cages under short days and long nights (8L:16D; L = 100 lux, D < 0.1 lux) for ∼3 weeks (19 days). Thereafter, they were divided into four groups (n = 6-9), and given ∼2 lux light either for the entire night (ZT 08-24; zeitgeber time 0 = time of light on; pattern A) or for 4 hr (pattern B), placed in 16 hr night such that its onset coincides with the onset of night (early night group, ZT 08-12), its end with the end of night (late night group, ZT 20-24), or the night was interrupted in the middle (midnight group, ZT 14-18). The results showed that bright light in entire night induced early onset of day activity and fragmented rest at night, however, if given at different phases of night, it made the days longer by delaying end (early night group) or advancing onset of daily activity (late night group). It also suppressed the melatonin levels and increased body temperature. These results suggest that bright light at night alters the perception of daylength and affects the underlying physiology. The findings may be useful in adopting a strategy for use of night light without disturbing species fitness in their environment.

Concurrent hypothalamic gene expression under acute and chronic long days: Implications for initiation and maintenance of photoperiodic response in migratory songbirds.

Hypothalamic expression of the thyroid hormone (TH) responsive gonadostimulatory (eya3, cga, tshß, dio2, dio3, gnrh, gnih) and neurosteroid pathway genes (androgen receptor [ar], aromatase [cyp19], estrogen receptor [er] α and ß) was examined in photosensitive redheaded buntings exposed to 2 (acute, experiment 1) or 12 (chronic, experiment 2) long days (16L:8D). Experiment 2 also included a photorefractory group. Acute long days caused a significant increase in eya3, cga, tshß, dio2 and gnrh and decrease in dio3 mRNA levels. eya3, cga and tshß expressions were unchanged after the chronic long days. We also found increased cyp19, erα and erß mRNA levels after acute, and increased cyp19 and decreased erß levels after the chronic long-day exposure. Photorefractory buntings showed expression patterns similar to that in the photosensitive state, except for high gnrh and gnih and low dio3 mRNA levels. Consistent with gene expression patterns, there were changes in fat deposition, body mass, testis size, and plasma levels of testosterone, tri-iodothyronine and thyroxine. These results show concurrent photostimulation of the TH-signalling and neurosteroid pathways, and extend the idea, based on differences in gene expression, that transitions in seasonal photoperiodic states are accomplished at the transcriptional levels in absolute photorefractory species.

Effect of complete and skeleton photoperiods in baya weaver, Ploceus philippinus.

A study was performed to investigate the effects of complete and skeleton photoperiods on gonadal stimulation in baya weaver, Ploceus philippinus. In August 2003, five groups of acclimatized birds (n = 7 each) were exposed to 6L: 13D: 1L: 4D, 6L: 6D: 1L: 11D, 11L: 13D and 13L: 11D. Birds were refractory and their exposure to 6L:13D:1L:4D, 6L:6D:1L:11D, 11L:13D and 13L:11D did not evoke any response. This clearly indicates that the birds were insensitive to the stimulatory effects of these photoperiods and perceived them as short days. An attempt was made to test the responsivity by subjecting all these groups to long day exposure after 16 weeks of experiment. Under 16L: 8D, birds did not show a response during the first 4 weeks of exposure (week 16 to week 20), but thereafter there was a significant photoinduction except 11L:13D (week 24), but the rate and magnitude was different. Until the end of the experiment (32 weeks), all groups of birds had maximum testicular volume except 11L:13D. Birds pre-exposed to 6L:13D:1L:4D, 6L:6D:1L:11D, 11L:13D and 13L:11D responded to 16L:8D, which suggests that these photoperiods helped the birds in recovering their photosensitivity. The results from this experiment indicate that baya weaver interprets a light period depending when the light falls relative to its endogenous circadian system.