[The impact of anxiety levels in male soldiers with infertility on stress coping strategies and the quality of fertility life].

OBJECTIVE: To explore and analyze the correlation between anxiety levels, coping strategies, and fertility quality of life in male soldiers with infertility. METHODS: A questionnaire survey was conducted on 480 male soldiers with infertility who visited the Reproductive Medicine Department of the Eastern Theater Command General Hospital from June 2022 to February 2023, analyze the impact of anxiety levels on stress coping strategies and fertility quality of life in male officers and soldiers with infertility. RESULTS: Self evaluation scale score is (43.06 ± 15.02), Fertility Quality of Life Scale score is （52.11 ± 36.68）, Trait Coping Style Questionnaire score is (48.45 ± 23.15). The relevant analysis results show that there is a negative correlation between the scores of the Self Rating Anxiety Scale and the Reproductive Quality of Life Scale, a positive correlation between the scores of the Self Rating Anxiety Scale and the Trait Coping Style Questionnaire, and a positive correlation between the scores of the Reproductive Quality of Life Scale and the Trait Coping Style Questionnaire. The results of multiple regression analysis showed that years of infertility, history of childbirth, anxiety level, and coping strategies entered the regression equation. The anxiety level of male officers and soldiers with infertility has a mediating effect on the relationship between stress coping styles and quality of life during childbirth. CONCLUSION: The mental health status of male officers and soldiers with infertility is poor, and their coping strategies and quality of life during childbirth are at a moderate to low level. This indicates that more attention should be paid to the special group of male officers and soldiers with infertility, and psychological intervention should be strengthened in routine treatment. Provide support from different perspectives to address concerns and enhance the combat effectiveness of the military.

[Colonization of group B streptococcus in the semen of chronic prostatitis patients of childbearing age and its influence on perinatal outcomes].

OBJECTIVE: To investigate the colonization of group B streptococcus (GBS) in the semen of chronic prostatitis patients of childbearing age and its influence on perinatal outcomes. METHODS: This retrospective study included 592 cases of chronic prostatitis and another 472 non-prostatitis healthy males as controls. We collected semen samples from the subjects for bacterial and Ureaplasma urealyticum (UU) culture and quantitative fluorescence PCR analysis of chlamydia trachomatis (CT) and GBS. According to the results of culture, we divided the patients into a GBS-positive and a GBS-negative group and compared the perinatal outcomes among different groups of subjects. RESULTS: The rate of GBS colonization in the semen of the chronic prostatitis patients was 11.8% (70/592). Bacteria were detected in the semen of 54.4% of the patients (322/592), mainly including GBS (21.7% ï¼»70/322ï¼½) and E coli (19.9% ï¼»64/322ï¼½), and in 7.8% of the healthy controls (37/472), Staphylococcus aureus comprising 83.8% (31/37), with statistically significant difference in the rate of bacteria detection between the two groups (P < 0.01). The incidence rate of adverse perinatal outcomes in the cases of successful pregnancy was significantly higher in the GBS-positive (32.8% ï¼»19/58ï¼½) than in the GBS-negative (22.0% ï¼»29/132ï¼½) and the healthy control group (2.2% ï¼»6/271ï¼½) (P < 0.05). CONCLUSIONS: The rate of GBS colonization is significantly increased in the semen of chronic prostatitis patients of childbearing age, and so is the incidence of adverse perinatal outcomes in the spouses of GBS-positive males. Importance should be attached to normalized screening of GBS in chronic prostatitis patients and to standardized prevention and intervention as well.

Density functional theory calculations and molecular dynamics simulations of the adsorption of biomolecules on graphene surfaces.

There is increasing attention in the unique biological and medical properties of graphene, and it is expected that biomaterials incorporating graphene will be developed for the graphene-based drug delivery systems and biomedical devices. Despite the importance of biomolecules-graphene interactions, a detailed understanding of the adsorption mechanism and features of biomolecules onto the surfaces of graphene is lacking. To address this, we have performed density functional theory (DFT) and molecular dynamics (MD) methods exploring the adsorption geometries, adsorption energies, electronic band structures, adsorption isotherms, and adsorption dynamics of l-leucine (model biomolecule)/graphene composite system. DFT calculations confirmed the energetic stability of adsorption model and revealed that electronic structure of graphene can be controlled by the adsorption direction of l-leucine. MD simulations further investigate the potential energy and van der Waals energy for the interaction processes of l-leucine/graphene system at different temperatures and pressures. We find that the van der Waals interaction between the l-leucine and the graphene play a dominant role in the adsorption process under a certain range of temperature and pressure, and the l-leucine molecule could be adsorbed onto graphene spontaneously in aqueous solution.