Detalhe da pesquisa
1.
Systematic interrogation of CRISPR antimicrobials in Klebsiella pneumoniae reveals nuclease-, guide- and strain-dependent features influencing antimicrobial activity.
Nucleic Acids Res
; 52(10): 6079-6091, 2024 Jun 10.
Artigo
em Inglês
| MEDLINE | ID: mdl-38661215
2.
Microbial defenses against mobile genetic elements and viruses: Who defends whom from what?
PLoS Biol
; 20(1): e3001514, 2022 01.
Artigo
em Inglês
| MEDLINE | ID: mdl-35025885
3.
Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response.
Mol Cell
; 65(1): 168-175, 2017 Jan 05.
Artigo
em Inglês
| MEDLINE | ID: mdl-28017588
4.
Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity.
Nucleic Acids Res
; 51(7): 3485-3496, 2023 04 24.
Artigo
em Inglês
| MEDLINE | ID: mdl-36929199
5.
Computational design of novel Cas9 PAM-interacting domains using evolution-based modelling and structural quality assessment.
PLoS Comput Biol
; 19(11): e1011621, 2023 Nov.
Artigo
em Inglês
| MEDLINE | ID: mdl-37976326
6.
Improving sequence-based modeling of protein families using secondary-structure quality assessment.
Bioinformatics
; 37(22): 4083-4090, 2021 11 18.
Artigo
em Inglês
| MEDLINE | ID: mdl-34117879
7.
Generating functional protein variants with variational autoencoders.
PLoS Comput Biol
; 17(2): e1008736, 2021 02.
Artigo
em Inglês
| MEDLINE | ID: mdl-33635868
8.
Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements.
Nucleic Acids Res
; 48(2): 748-760, 2020 01 24.
Artigo
em Inglês
| MEDLINE | ID: mdl-31745554
9.
On-target activity predictions enable improved CRISPR-dCas9 screens in bacteria.
Nucleic Acids Res
; 48(11): e64, 2020 06 19.
Artigo
em Inglês
| MEDLINE | ID: mdl-32352514
10.
Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels.
Methods
; 172: 61-75, 2020 02 01.
Artigo
em Inglês
| MEDLINE | ID: mdl-31377338
11.
Cas9 specifies functional viral targets during CRISPR-Cas adaptation.
Nature
; 519(7542): 199-202, 2015 Mar 12.
Artigo
em Inglês
| MEDLINE | ID: mdl-25707807
12.
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors.
PLoS Genet
; 14(11): e1007749, 2018 11.
Artigo
em Inglês
| MEDLINE | ID: mdl-30403660
13.
Conditional tolerance of temperate phages via transcription-dependent CRISPR-Cas targeting.
Nature
; 514(7524): 633-7, 2014 Oct 30.
Artigo
em Inglês
| MEDLINE | ID: mdl-25174707
14.
Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes.
Mol Syst Biol
; 14(3): e7899, 2018 03 08.
Artigo
em Inglês
| MEDLINE | ID: mdl-29519933
15.
Microbial-derived products as potential new antimicrobials.
Vet Res
; 49(1): 66, 2018 07 31.
Artigo
em Inglês
| MEDLINE | ID: mdl-30060765
16.
Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
Nucleic Acids Res
; 44(9): 4243-51, 2016 05 19.
Artigo
em Inglês
| MEDLINE | ID: mdl-27060147
17.
Impact of Different Target Sequences on Type III CRISPR-Cas Immunity.
J Bacteriol
; 198(6): 941-50, 2016 Jan 11.
Artigo
em Inglês
| MEDLINE | ID: mdl-26755632
18.
Adapting to new threats: the generation of memory by CRISPR-Cas immune systems.
Mol Microbiol
; 93(1): 1-9, 2014 Jul.
Artigo
em Inglês
| MEDLINE | ID: mdl-24806524
19.
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
Nucleic Acids Res
; 41(15): 7429-37, 2013 Aug.
Artigo
em Inglês
| MEDLINE | ID: mdl-23761437
20.
The integron integrase efficiently prevents the melting effect of Escherichia coli single-stranded DNA-binding protein on folded attC sites.
J Bacteriol
; 196(4): 762-71, 2014 Feb.
Artigo
em Inglês
| MEDLINE | ID: mdl-24296671