RESUMO
This paper reports a one-step method to fabricate highly porous micropatterned 2-D scaffold sheets. The scaffold sheets have high glucose diffusion, indicating that the porosity and pore morphology of the scaffolds are viable with respect to nutrient transport, and a micropattern for cell alignment. HUVEC culturing proved that the scaffold sheets are suitable for cell culturing. More extensive culturing experiments with mouse myoblasts, C2C12, and mouse osteoblasts, MC3T3, showed that tissue organization can be controlled; the micropattern design affects the extent of cell alignment and tissue formation. Cells are favorably settled in the micropattern and even at higher confluence levels, when the cells start to overgrow the ridges of the micropattern, these cells align themselves in the direction of the micropattern. Preliminary multi-layer stacking experiments indicate that the 2-D scaffold sheets are very promising as basis for building 3-D scaffolds.
Assuntos
Materiais Biocompatíveis/química , Engenharia Tecidual/métodos , Células 3T3 , Animais , Técnicas de Cultura de Células/métodos , Linhagem Celular , Endotélio Vascular/citologia , Ácido Láctico/química , Camundongos , Mioblastos/metabolismo , Osteoblastos/metabolismo , Poliésteres , Polímeros/química , Porosidade , Solventes , Propriedades de SuperfícieRESUMO
Occluding artery disease causes a high demand for bioartificial replacement vessels. We investigated the combined use of biodegradable and creep-free poly (1,3-trimethylene carbonate) (PTMC) with smooth muscle cells (SMC) derived by biochemical or mechanical stimulation of adipose tissue-derived stromal cells (ASC) to engineer bioartificial arteries. Biochemical induction of cultured ASC to SMC was done with TGF-ß1 for 7d. Phenotype and function were assessed by qRT-PCR, immunodetection and collagen contraction assays. The influence of mechanical stimulation on non-differentiated and pre-differentiated ASC, loaded in porous tubular PTMC scaffolds, was assessed after culturing under pulsatile flow for 14d. Assays included qRT-PCR, production of extracellular matrix and scanning electron microscopy. ASC adhesion and TGF-ß1-driven differentiation to contractile SMC on PTMC did not differ from tissue culture polystyrene controls. Mesenchymal and SMC markers were increased compared to controls. Interestingly, pre-differentiated ASC had only marginal higher contractility than controls. Moreover, in 3D PTMC scaffolds, mechanical stimulation yielded well-aligned ASC-derived SMC which deposited ECM. Under the same conditions, pre-differentiated ASC-derived SMC maintained their SMC phenotype. Our results show that mechanical stimulation can replace TGF-ß1 pre-stimulation to generate SMC from ASC and that pre-differentiated ASC keep their SMC phenotype with increased expression of SMC markers.
Assuntos
Tecido Adiposo/citologia , Técnicas de Cultura de Células/métodos , Miócitos de Músculo Liso/citologia , Engenharia Tecidual/métodos , Fenômenos Biomecânicos , Adesão Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Humanos , Fenótipo , Células Estromais/citologia , Alicerces Teciduais , Fator de Crescimento Transformador beta1/farmacologiaRESUMO
Phase separation micromolding (PSmicroM) is a versatile microfabrication technique that can be used to structure a very broad range of polymers, including block copolymers and biodegradable and conductive polymers without the need for clean-room facilities. By incorporating a subsequent process step, carbon, ceramic, and metallic microstructures can also be fabricated from a polymeric or hybrid precursor. The replication process is straightforward and cost-effective. It relies on phase separation of a polymer solution while in contact with a structured mold. Intrinsic shrinkage during the phase separation facilitates the release of the replica from the mold, which increases the reliability of the process even at small feature sizes, thin polymer films, or high aspect ratios. Under suitable circumstances perforation of the polymer film can be obtained, resulting in completely open "through" microstructures. Furthermore, porosity can be introduced in a microstructure, which may result in unknown functionalities.
Assuntos
Microquímica/instrumentação , Microquímica/métodos , Nanotecnologia/instrumentação , Nanotecnologia/métodos , Polímeros/química , Álcoois/química , Materiais Biocompatíveis/química , Carbono , Cerâmica/química , Cristalização , Dimetil Sulfóxido/química , Furanos/química , Temperatura Alta , Teste de Materiais , Polietileno/química , Pirrolidinonas/química , Propriedades de Superfície , TemperaturaRESUMO
Hemodialysis is a commonly used blood purification technique in patients requiring kidney replacement therapy. Sorbents could increase uremic retention solute removal efficiency but, because of poor biocompatibility, their use is often limited to the treatment of patients with acute poisoning. This paper proposes a novel membrane concept for combining diffusion and adsorption of uremic retention solutes in one step: the so-called mixed-matrix membrane (MMM). In this concept, adsorptive particles are incorporated in a macro-porous membrane layer whereas an extra particle-free membrane layer is introduced on the blood-contacting side of the membrane to improve hemocompatibility and prevent particle release. These dual-layer mixed-matrix membranes have high clean-water permeance and high creatinine adsorption from creatinine model solutions. In human plasma, the removal of creatinine and of the protein-bound solute para-aminohippuric acid (PAH) by single and dual-layer membranes is in agreement with the removal achieved by the activated carbon particles alone, showing that under these experimental conditions the accessibility of the particles in the MMM is excellent. This study proves that the combination of diffusion and adsorption in a single step is possible and paves the way for the development of more efficient blood purification devices, excellently combining the advantages of both techniques.
Assuntos
Sangue , Membranas Artificiais , Injúria Renal Aguda/sangue , Injúria Renal Aguda/terapia , Adsorção , Creatinina/sangue , Creatinina/isolamento & purificação , Difusão , Humanos , Microscopia Eletrônica de Varredura , Diálise Renal , Ácido p-Aminoipúrico/sangue , Ácido p-Aminoipúrico/isolamento & purificaçãoRESUMO
Scaffolds with a nano-fibrous morphology are favored for certain tissue engineering applications as this morphology mimics the tissue's natural extracellular matrix secreted by the cells, which consists of mainly collagen fibers with diameters ranging from 50 to 400 nm. Porous poly(L-lactide) (PLLA) scaffolds obtained by phase inversion methods generally have a solid-wall pore morphology. In contrast, this work presents a facile method to fabricate highly porous and highly interconnected nano-fibrous scaffold sheets by phase inversion using PLLA of very high molecular weight (5.7x10(5) g mol(-1)). The scaffold sheets consist of nano-fibers within the desired range of 50-500 nm. When applying phase separation micromolding as a fabrication method besides the porous nano-fibrous morphology, an additional topography can be introduced into these sheets. Culturing of C2C12 pre-myoblasts on these nano-fibrous sheets reveals very good cell adhesion, morphology and proliferation. Excellent alignment of the cells is induced by fabrication of 25 microm wide microchannels in these sheets. These results warrant further evaluation of these sheets as tissue engineering scaffolds.
Assuntos
Nanoestruturas , Poliésteres/química , Animais , Linhagem Celular , Microscopia Eletrônica de Varredura , Microscopia de Fluorescência , Peso MolecularRESUMO
Using phase separation micromolding (PSmicroM) we developed porous micro-patterned sheets from amorphous poly(1,3-trimethylene carbonate) (PTMC). The use of these PTMC sheets can be advantageous in tissue engineering applications requiring highly flexible constructs. Addition of poly(ethylene oxide) (PEO) in various amounts to PTMC casting solutions provides PTMC sheets with tailored porosity and pore sizes in the range 2-20 microm. The pore-forming effect of PEO during the phase separation process is evaluated and glucose transport measurements show that the pores are highly interconnected. Additionally, tailoring the micro-pattern design yields PTMC sheets with various surface topographies. Cell culturing experiments with C2C12 pre-myoblasts revealed that cell attachment and proliferation on these sheets is relatively high and that the micro-pattern topography induces a clearly defined cell organization.