RESUMO
The complement regulatory protein (CRP) of Trypanosoma cruzi is a surface glycoprotein which confers to the infectious trypomastigote forms a protection against the lytic activity of the host complement. CRP belongs to the large family of the trans-sialidase-like proteins and its sequence is highly similar to those of the flagellar FL-160 and chronic exoantigen proteins, encoded by a multigene family. To further define the gene family encoding the CRP, we investigated the protein diversity among several strains of T. cruzi through the sequencing of trypomastigote transcripts, and used a phylogenetic analysis based on the multiple alignment of these proteins with the top scoring sequences detected by a database sequence homology search. Intrastrain variations in CRP sequences revealed the existence of several copies per strain. The interstrain variability of CRP was consistent with the genetic subdivisions of T. cruzi into lineages and discrete typing units. The phylogenetic analysis based on a 227 amino acid alignment of CRP sequences with the 200 putative proteins retrieved from the protein databases (including the sequences from the T. cruzi genome project) revealed that the CRP sequences clustered with the FL-160 proteins into a monophyletic group characterized by the presence of the 12 amino acid mimicry epitope that mimics nervous tissues. The phylogeny did not differentiate between the CRP and the FL-160 proteins. The identification of this group of CRP-like proteins and the high sequence similarity observed within it open up new prospects for the exploration of the localization, structure and function of these proteins and a better understanding of their involvement in key aspects of host-parasite interactions, such as the resistance to the complement. This work provides also information for the T. cruzi genome annotation of the trans-sialidase-like putative proteins.
Assuntos
Variação Genética , Estágios do Ciclo de Vida/genética , Glicoproteínas de Membrana/genética , Proteínas de Protozoários/genética , Trypanosoma cruzi/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Análise por Conglomerados , Genes de Protozoários , Glicoproteínas/química , Dados de Sequência Molecular , Família Multigênica , Neuraminidase/química , Filogenia , Trypanosoma cruzi/crescimento & desenvolvimentoRESUMO
Chagas disease in the state of Jalisco, Mexico was described for the first time in 1967; however, knowledge on the disease remains in a slow process. Between 1967 and 2006, the disease was described in its acute and chronic forms. The vector species have been identified, and the parasite Trypanosoma cruzi has been isolated and genetically characterized. Also, the magnitude of the infection in humans has been determined through serological studies of different populations as well as of blood donors. The up-to-dateness of knowledge of the disease in the state of Jalisco, unveils a necessity of increased research on the epidemiology of Chagas disease as well as on clinical studies to assess the health of individuals and the populations.
Assuntos
Doença de Chagas/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Doadores de Sangue , Cardiomiopatia Chagásica/epidemiologia , Doença de Chagas/complicações , Doença de Chagas/transmissão , Criança , Acalasia Esofágica/epidemiologia , Acalasia Esofágica/etiologia , Feminino , Humanos , Lactente , Insetos Vetores/parasitologia , Conhecimento , Masculino , México/epidemiologia , Pessoa de Meia-Idade , Estudos Soroepidemiológicos , Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificação , Adulto JovemRESUMO
Antigenic similarities between salivarian trypanosomes are known for a long time, but similarities between salivarian and stercorarian trypanosomes have been very little investigated. Phylogenetically, these genus and species appear to be far. However, in a preliminary work we had shown strong reactions of chagasic human sera using T. evansi antigens in Western-blotting and ELISA. In the current work an ELISA test using T. evansi crude antigens was probed with one hundred and two sera of chagasic Bolivian patients previously diagnosed which presented different pathologies. The sensitivity of the ELISA T. evansi was 92.6% similar to that of ELISA T. cruzi. The specificity evaluated using 20 sera of patients infected by Leishmania sp. reaches a comparable value of that obtained with the T. cruzi immunofluorescent assay. Finally, the sensitivity and the specificity of the ELISA T. evansi were not really different from conventional serology of Chagas. In spite of their taxonomic position in various sections and their old divergence, these observations prove a strong antigenic community between T. cruzi and T. evansi. Consequently, the common antigens which remain to be characterized, could be an alternative source of antigen for the detection of antibodies against T. cruzi. Given that T. evansi seems to have strong antigenic communities with the majority of the pathogenic current trypanosomoses of mammals, it is very attractive to identify and characterize these highly conserved antigens which could be suitable targets to develop tools for diagnosis, prophylaxy and chemotherapy against several human and animal trypanosomoses.
Assuntos
Antígenos de Protozoários , Doença de Chagas/diagnóstico , Trypanosoma cruzi/isolamento & purificação , Animais , Anticorpos Antiprotozoários , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , HumanosRESUMO
American trypanosomiasis or Chagas disease is endemic in Latin America and caused by the flagellate Trypanosoma cruzi, which exhibits broad genetic variation. In various areas, the transmission of Chagas disease is ensured by sylvatic vectors, mainly carrying the evolutionary lineage I of T. cruzi. Despite its epidemiological importance, this lineage is poorly studied. Here, we investigated the genetic variability and the phylogenetic relationships within T. cruzi I using sequences of the non-transcribed spacer of miniexon genes. The variability was firstly analysed between 10 repeats of spacer-miniexon genes in two strains of T. cruzi I and in the CL Brener strain, showing lower intra-strain variability than inter-strain. Furthermore, the phylogenetic analysis of 19 T. cruzi I strains (49 copies in total) clusters the copies into at least three groups. Two evolutionary phenomena can be proposed to explain the partition of the strains: (i) an association between strains and Didelphis sp. hosts and (ii) geographical clustering between the North American and South American strains. Thereby, the miniexon gene is an attractive marker to establish the phylogeny of lineage I and explore relationships between T. cruzi and mammal hosts.
Assuntos
DNA Intergênico/genética , Éxons/genética , Trypanosoma cruzi/classificação , Trypanosoma cruzi/genética , Animais , Sequência de Bases , Dados de Sequência Molecular , Filogenia , Polimorfismo GenéticoRESUMO
An examination of peridomestic area organization and triatomine collection in an endemic village for Chagas disease (Jalisco State) identified the habitat of Triatoma longipennis (dominant species) and the risk factors of peridomestic infestation. In 100 visited peridomestic areas, 369 structures (permanent, temporary, and natural) were submitted to active manual research of triatomines. Storage shelters had a higher infestation of T. longipennis than piles of brick and tile; baked clay material had higher degrees of infestation than others. The secondary species Triatoma barberi shares a wide range of peridomicilary habitats with T. longipennis. Peridomestic area infestation risks (evaluated with multivariate logistic regression analysis) are number of closed storage shelters, number of brick and tile piles, number of houses per peridomestic areas, and distance of peridomicile from natural environment. Because both species present great adaptability to different artificial habitats, strategies of control must involved improving the overall management of peridomestic areas to prevent stable colonization.
Assuntos
Doença de Chagas/transmissão , Insetos Vetores , Triatoma , Animais , Humanos , México , Fatores de Risco , População RuralRESUMO
Infestation of peridomiciles is likely a major risk factor for Chagas disease transmission in Jalisco state, Mexico. An entomological and serological survey of a typical village was conducted between July and September 2003. The peridomestic areas of 100 dwellings were visited and triatomines were searched manually in 369 potential sites. A total of 1821 Triatoma longipennis (93.2%) or Triatoma barberi was captured. Both species frequently occurred in sympatry. The infestation index was 60% for T. longipennis and 16% for T. barberi. T. longipennis occurred throughout the village. Colonization indices were high for T. longipennis (93%) and T. barberi (75%), suggesting that both species have adapted to peridomestic habitats. The bug population size was larger for T. longipennis than for T. barberi. Five very large colonies of T. longipennis were recorded whereas only 1 or 2 bugs were observed in 38% of the positive sites, which suggests intense dispersal activity. Both species exhibited high infection prevalence with Trypanosoma cruzi (46%). Only T. cruzi lineage I was detected. Human seroprevalence was 1.8%. This study serves as an entomological overview of peridomiciliar triatomine colonization in a Mexican village and highlights the current risk of Chagas disease transmission.
Assuntos
Doença de Chagas/transmissão , Triatoma , Trypanosoma cruzi/patogenicidade , Adolescente , Adulto , Animais , Doença de Chagas/epidemiologia , Criança , Pré-Escolar , Feminino , Habitação , Humanos , Insetos Vetores , Masculino , México/epidemiologia , Pessoa de Meia-Idade , População RuralRESUMO
Feeding sources of triatomine vectors (Triatoma longipennis) collected in peridomiciles in Mexico were identified by a heteroduplex assay developed with triatomine blood meals. Trypanosoma cruzi parasites were also characterized in the same blood meal samples by multiplex-polymerase chain reaction assay of mini-exon gene inter-genic regions. The main blood meal source was from rats, but the bugs were able to feed on a wide variety of hosts, and human blood meals were identified. Trypanosoma cruzi was the only flagellate species identified in the blood meals. All populations belong to the T. cruzi I lineage, a result that is consistent with the previously assumed predominance of this lineage in Mexico. This combination of blood meal and T. cruzi lineage identification provides a powerful tool for understanding T. cruzi transmission cycles.
Assuntos
Doença de Chagas/transmissão , Insetos Vetores/fisiologia , Triatoma/fisiologia , Trypanosoma cruzi/classificação , Animais , Doença de Chagas/parasitologia , Comportamento Alimentar , Feminino , Humanos , Insetos Vetores/parasitologia , Masculino , México/epidemiologia , Reação em Cadeia da Polimerase/métodos , Ratos , Triatoma/parasitologiaRESUMO
Thirty-five Bolivian children (5-10 years of age) seropositive for infection with T. cruzi underwent specific chemotherapy with benznidazole. Before treatment, 57.1% had a positive parasitologic diagnosis. Some patients presented an early conversion by polymerase chain reaction of blood samples, while others were still positive four and seven months after the end of the treatment, which indicated an absence of parasite clearance. Strain typing showed that most patients were infected by a mixture of clones I and II of T. cruzi. Serologic conversion in conventional tests and antibodies to shed acute-phase antigen were observed in two and four patients, respectively. For the other patients, the average rate of antibody decay was half the initial rate. The parasitologic and serologic data indicated that chemotherapy acts throughout the course of infection in a long-lasting process in which the decrease of specific antibody production is related to the reduction of the live parasite load.
Assuntos
Nitroimidazóis/uso terapêutico , Reação em Cadeia da Polimerase/métodos , Tripanossomicidas/uso terapêutico , Trypanosoma cruzi/isolamento & purificação , Tripanossomíase/tratamento farmacológico , Animais , Anticorpos Antiprotozoários/sangue , Bolívia , Criança , Pré-Escolar , Humanos , Especificidade da Espécie , Trypanosoma cruzi/classificação , Tripanossomíase/imunologia , Tripanossomíase/parasitologiaRESUMO
Domestic and peridomestic triatomine populations were collected in three rural Mexican communities of Jalisco, Nayarit and Zacatecas states. Triatoma longipennis and T. picturata (Phyllosoma complex) were the principal species unequally distributed in the villages: T. longipennis was the main species in two communities and T. picturata in the third one. Peridomestic infestation and colonization indexes were remarkably high ranging from 26.1% to 50% and from 58.3% to 85.7%, respectively. Moreover, domestic (indoor) infestation was observed in only one of the communities infested by T. longipennis. The preliminary study of temporal variation indicates increasing trend of the triatomine population and infestation rates during the dry season. Triatomine infection rates ranged from 41.2% to 60.2% and all the flagellate isolates were assigned to T. cruzi I. The majority of the dwellings were built with modern building materials and the sanitary conditions were generally good. High peridomestic infestations must be considered as a risk factor of Chagas disease transmission and further studies are needed to better understand the peridomestic conditions favoring the establishment of the triatomines. The contribution of such study to enlarger knowledge of epidemiological features of Chagas disease in Mexico is considered.
Assuntos
Doença de Chagas/parasitologia , Doença de Chagas/transmissão , Vetores de Doenças , Triatoma/fisiologia , Trypanosoma cruzi/parasitologia , Animais , Doença de Chagas/epidemiologia , Habitação , Humanos , México/epidemiologia , Trypanosoma cruzi/fisiologiaRESUMO
BACKGROUND: Chagas disease is a major public health problem in Latin America. Its etiologic agent, Trypanosoma cruzi, is mainly transmitted through the contaminated faeces of blood-sucking insects called triatomines. Triatoma infestans is the main vector in various countries in South America and recently, several foci of wild populations of this species have been described in Bolivia and other countries. These wild populations are suspected of affecting the success of insecticide control campaigns being carried out in South America. To assess the risk that these T. infestans populations pose to human health, it is helpful to determine blood meal sources. METHODS: In the present work, blood meals were identified in various Bolivian wild T. infestans populations and in three specific areas, in both wild and intra-peridomestic populations to assess the links between wild and domestic cycles of T. cruzi transmission. PCR-HDA and sequencing of Cytb gene were used to identify these blood meal sources. RESULTS AND DISCUSSION: Fourteen vertebrate species were identified as wild blood meal sources. Of those, the most prevalent species were two Andean endemic rodents, Octodontomys gliroides (36%) and Galea musteloides (30%), while humans were the third most prevalent source (18.7%). Of 163 blood meals from peridomestic areas, more than half were chickens, and the others were generally domestic animals or humans. Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission. CONCLUSION: The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment. These results support the risk to human health posed by wild populations of T. infestans.
Assuntos
Animais Domésticos/parasitologia , Animais Selvagens/parasitologia , Doença de Chagas/veterinária , Insetos Vetores/parasitologia , Triatoma/parasitologia , Trypanosoma cruzi/fisiologia , Animais , Animais Domésticos/sangue , Animais Domésticos/classificação , Animais Selvagens/sangue , Animais Selvagens/classificação , Sangue/parasitologia , Bolívia/epidemiologia , Doença de Chagas/epidemiologia , Doença de Chagas/parasitologia , Doença de Chagas/transmissão , Humanos , Insetos Vetores/fisiologia , Triatoma/fisiologia , Trypanosoma cruzi/genética , Trypanosoma cruzi/isolamento & purificaçãoRESUMO
The intracellular protozoan parasite Trypanosoma cruzi is the etiological agent of Chagas' disease. We have previously characterized a T. cruzi virulence factor named Tc52 sharing structural and functional properties with the thioredoxin and glutaredoxin protein family. Single mutant parasite clones (Tc52(+/-)) exhibiting low virulence in vitro and in vivo were obtained by targeted Tc52 gene replacement. In this report, we have extended our study to analyze the immune response and the disease phenotype in Tc52(+/-)-infected BALB/c mice, during the acute and chronic phases of the disease. Significantly lower parasitemia were found in Tc52(+/-)-infected mice, as compared to wild-type parasite (WT)-infected ones. However, the expansion of all classes of lymphocytes and macrophages was similar for both clones. Furthermore, except for IgG2b levels which were higher in the case of WT-infected mice, all classes of Ig presented no significant difference for WT and Tc52(+/-)-infected animals. Interestingly, a lack of suppression of IL-2 production and of T-cell proliferation inhibition was observed in the case of spleen cells from Tc52(+/-)-infected mice. Finally, the pattern of inflammation process was different and characterized as diffused in the case of Tc52(+/-)-infected mice, or presenting numerous foci in the case of WT-infected mice. Localization of the Tc52 protein in tissue sections and infected heart cell primary cultures by immunofluorescence and immunogold labeling, respectively, revealed the presence of Tc52 at the amastigote surface and associated to aggregates within host cell vesicles. Taken together, these results reinforce the notion of Tc52 being a virulence factor playing a role in the phenotype of the immune response associated to the infection and on the course of the disease.
Assuntos
Doença de Chagas/imunologia , Proteínas de Protozoários/genética , Proteínas de Protozoários/fisiologia , Trypanosoma cruzi/genética , Trypanosoma cruzi/patogenicidade , Animais , Anticorpos Antiprotozoários/sangue , Apoptose , Células Cultivadas , Doença de Chagas/parasitologia , Doença de Chagas/patologia , Citocinas/biossíntese , Citocinas/genética , Ensaio de Imunoadsorção Enzimática , Marcação de Genes , Coração/parasitologia , Imunoglobulinas/sangue , Interferon gama/biossíntese , Camundongos , Camundongos Endogâmicos BALB C , Músculo Esquelético/química , Músculo Esquelético/parasitologia , Músculo Esquelético/patologia , Miocárdio/química , Miocárdio/patologia , Proteínas de Protozoários/análise , Proteínas de Protozoários/imunologia , Baço/citologia , Baço/imunologia , Trypanosoma cruzi/imunologia , Trypanosoma cruzi/fisiologiaRESUMO
Granite rocks is a very abundant material in Mexico because they are used habitually as borders for fields. The current work established the significance of this ecotype as a colonization site for triatomines of the Phyllosoma complex. Seven sites, arbitrary selected, in San Martin de Hidalgo municipality (Mexico) were investigated in April 2002 by using 210 mouse bait-traps left during the night in wall hollows. One hundred and seventy-two triatomines of all life stages were collected from the seven sites. Triatomines adhered to the tape that covered the traps or were found near them, and 36.6% of the traps collected triatomines. The principal species was Triatomia longipennis Usinger (1939) and low numbers of Triatoma picturata Usinger (1939) were found. The nymphal population was very young, probably corresponding to the reproductive period in April (end of the dry season). The infection rate of the triatomines for Trypanosoma cruzi was 49%. Because collecting triatomines in the field is laborious and time-consuming, the mouse bait-trap method found to be practical to assess the population of triatomines within a sylvatic habitat.
Assuntos
Triatominae/crescimento & desenvolvimento , Animais , Meio Ambiente , Geografia , Fenômenos Geológicos , Geologia , México , CamundongosRESUMO
We analyzed triatomine blood feeding patterns to evaluate the role of peridomiciles in Trypanosoma cruzi transmission at the rural village of Tepehuaje de Morelos at Jalisco State, Mexico (1999). A total of 206 bugs were collected in 11 out of 26 households (42.3%). Nymphs predominated in the collections (64.9% of the total). Except for one Triatoma barberi female, a species that belongs to the protracta species complex, all adults were Triatoma longipennis, a species of the phyllosoma complex. Triatomines were exclusively present in peridomestic sites mainly piles of tiles and bricks, and none were found indoors. Overall infection rate was 56.6% and no significant differences (P > 0.05) were observed between nymphs and adults or males and females. Identified blood meals were chicken (29.4%), opossum (20.9%), pig (24.5%), murid (20.9%), dog (3.5%), and armadillo (0.7%). No gut content reacted against anti-human, anti-bovine, anti-rabbit, and anti-cat sera. In contrast to fifth nymphs and adults, 87% of the small nymphs fed on one host, indicating that they are less mobile than other stages. Most fifth nymphs and adults fed on domestic hosts, while small nymphs mainly fed on opossum and murid. Infection blood-meal indexes were around 50% for single meals on opossum and murid, stressing their importance as trypanosome donors. Peridomiciles in Tepehuaje could be regarded as interaction sites among domestic and wild and synanthropic mammals and triatomines, which would facilitate circulation of the same T. cruzi strains between domestic and sylvatic cycles. Stone-made walls and building materials, which hold synanthropic rodents and opossums, should be considered as targets for vector control measures.
Assuntos
Meio Ambiente , Comportamento Alimentar/fisiologia , Triatoma/fisiologia , Animais , Animais Domésticos/parasitologia , Animais Selvagens/parasitologia , Geografia , MéxicoRESUMO
Triatoma infestans, the major vector of Chagas disease south of the Amazon in South America, has a large distribution of wild populations, contrary to what has previously been stated. These populations have been suspected of being the source of reinfestation of human habitats and could impede the full success of vector control campaigns. This study examined gene flow between intra-peridomestic populations and wild populations collected in the surround areas in three Andean localities in Bolivia. The populations were defined according to temporal, ecological, and spatial criteria. After DNA extraction from the legs of each insect, the samples were analyzed using seven microsatellite markers. First, the analysis of molecular variance (AMOVA) detected an absence of differentiation between wild and intra-peridomestic populations, although strong structuring was observed between the populations within each environment. Then for some populations, the Bayesian method of assignment to inferred populations showed very similar assignment patterns of the members of wild or intra-peridomestic populations in each locality. Finally, the detection of the first-generation migrants within the different populations provided evidence of insect displacement from the wild to the intra-peridomestic environment. This result indicates that, after control campaigns in the Andes, controlling this new paradigm of vector transmission risk stemming from the invasion of human habitats by wild populations of T. infestans requires long-term maintenance of public monitoring to keep the risk at a minimal level. Since wild populations of T. infestans have also been detected elsewhere in Argentina, Paraguay, and Chile, there is an urgent need to take these populations into account in future monitoring of Chagas disease transmission.
Assuntos
Ecossistema , Triatoma/genética , Animais , Bolívia , Feminino , Loci Gênicos/genética , Variação Genética , Genótipo , Geografia , Humanos , Masculino , Repetições de Microssatélites/genética , Filogenia , Dinâmica PopulacionalRESUMO
We analyzed the food sources of Bolivian wild Triatoma infestans (the main vector of Chagas disease in this country), to assess the role of these populations in the epidemiological context of Chagas disease. Ninety-eight blood meals were identified by heteroduplex assay and sequencing. Most of them were from wild mammals but surprisingly 27 were from humans. This brings to light the occurrence of human-vector contacts at risk of Trypanosoma cruzi transmission in the wild environment by highly infected insects.
Assuntos
Doença de Chagas/transmissão , Comportamento Alimentar/fisiologia , Insetos Vetores/fisiologia , Triatoma/fisiologia , Animais , Animais Selvagens/classificação , Animais Selvagens/genética , Sequência de Bases , Bolívia , Citocromos b/genética , DNA/sangue , DNA/classificação , DNA/genética , Humanos , Insetos Vetores/química , Mamíferos/classificação , Mamíferos/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Triatoma/química , Trypanosoma cruziRESUMO
Trypanosoma cruzi, the causative agent of Chagas disease, is subdivided into six discrete typing units (DTUs; TcI-TcVI) of which TcI is ubiquitous and genetically highly variable. While clonality is the dominant mode of propagation, recombinant events play a significant evolutive role. Recently, foci of wild Triatoma infestans have been described in Bolivia, mainly infected by TcI. Hence, for the first time, we evaluated the level of genetic exchange within TcI natural potentially panmictic populations (single DTU, host, area and sampling time). Seventy-nine TcI stocks from wild T. infestans, belonging to six populations were characterized at eight microsatellite loci. For each population, Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), and presence of repeated multilocus genotypes (MLG) were analyzed by using a total of seven statistics, to test the null hypothesis of panmixia (H0). For three populations, none of the seven statistics allowed to rejecting H0; for another one the low size did not allow us to conclude, and for the two others the tests have given contradictory results. Interestingly, apparent panmixia was only observed in very restricted areas, and was not observed when grouping populations distant of only two kilometers or more. Nevertheless it is worth stressing that for the statistic tests of "HWE", in order to minimize the type I error (i. e. incorrect rejection of a true H0), we used the Bonferroni correction (BC) known to considerably increase the type II error ( i. e. failure to reject a false H0). For the other tests (LD and MLG), we did not use BC and the risk of type II error in these cases was acceptable. Thus, these results should be considered as a good indicator of the existence of panmixia in wild environment but this must be confirmed on larger samples to reduce the risk of type II error.
Assuntos
Triatoma/parasitologia , Trypanosoma cruzi/isolamento & purificação , Animais , Bolívia , Variação Genética , Interações Hospedeiro-Parasita , Desequilíbrio de Ligação , Repetições de Microssatélites/genética , Triatoma/genéticaRESUMO
The identification of blood meals in vectors contributes greatly to the understanding of interactions between vectors, microorganisms and hosts. The aim of the current work was to complement the validation of cytochrome b (Cytb) heteroduplex assay (HDA) previously described, and to add the sequencing of the Cytb gene of some samples for the identification of blood meals in triatomines. Experimental feedings of reared triatomines helped to clarify the sensitivity of the HDA. Moreover, the sequencing coupled with the HDA, allowed the assessment of the technique's taxonomic level of discrimination. The primers used to produce DNA fragments of Cytb genes for HDA had a very high sensitivity for vertebrate DNAs, rather similar for mammals, birds and reptiles. However, the formation of heteroduplex depended on blood meal's quality rather than its quantity; a correlation was observed between blood meals' color and the positivity of HDA. HDA electrophoresis profiles were reproducible, and allowed the discrimination of blood origins at the species level. However, in some cases, intraspecific variability of Cytb gene generated different HDA profiles. The HDA based on comparison of electrophoresis profiles is a very useful tool for screening large samples to determine blood origins; the subsequent sequencing of PCR products of Cytb corresponding to different HDA profiles allowed the identification of species whatever the biotope in which the vectors were captured.
Assuntos
Sangue , Citocromos b/genética , Triatominae/química , Animais , Fragmentação do DNA , Primers do DNA , Análise Heteroduplex , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
The originally wild species of the Meccus complex are important vectors of Chagas disease in Mexico. In West Mexico, Meccus longipennis plays an important epidemiological role. To understand the genetic structure of the domestic and wild populations of this species, a preliminary study with five polymorphic microsatellite loci was conducted. The population genetics analysis showed high structuring between peridomestic biotopes, with breeding subunits detected in a single peridomestic structure. In the wild environment, two genetic patterns were observed according to the biotope, possible breeding subunits in large rocky formations and a larger panmictic unit in agropastoral areas, suggesting considerable dispersal of bugs in this biotope. Moreover, the discovery of two foci of wild populations at the edge of Guadalajara city raises the question of new urban areas where the phenomenon of bug incursions into households could constitute a risk of transmission of Chagas disease.
Assuntos
Doença de Chagas/epidemiologia , Insetos Vetores/genética , Triatominae/genética , Animais , Doença de Chagas/transmissão , Evolução Molecular , Variação Genética , Genótipo , Humanos , México/epidemiologia , Repetições de Microssatélites , Filogenia , População/genética , Triatominae/classificaçãoRESUMO
Trypanosoma cruzi, the agent of Chagas disease, is usually subdivided into six discrete typing units (DTUs), TcI to TcVI, among which TcI and TcV are most common in human infections in Bolivia. Multilocus microsatellite typing (MLMT) was selected to further explore the structure of the natural populations belonging to these DTUs. The analysis showed that microsatellite clustering does not fully match the six DTUs, but it is relevant for the within DTUs analyses. Population genetics analysis was conducted on 11 relevant subsamples of stocks from Bolivia and Peru, belonging to TcI (6) and TcV (5), defined by four criterions: DTU, vector species, geographic origin, and date of isolation. Most TcV strains presented the same multilocus genotype over all subsamples with the puzzling characteristic that five loci were heterozygous and the other five homozygous. In TcI, four clusters were defined according to the vector species. Most of them appeared in agreement with clonal propagation (stocks isolated from Triatoma infestans and Triatoma sordida), while a few highly homozygous stocks (e.g. those isolated from Rhodnius stali) suggested that scarce sex events can occur. The poor role played by spatio-temporal factors in describing the observed genetic diversity suggested that ecology, in particular as regard to host played a significant role. These results highlight the extreme heterogeneity of T. cruzi and suggest that further population genetics surveys will need to target the most possible precise spatio-temporal and ecological scales.
Assuntos
Doença de Chagas/parasitologia , Repetições de Microssatélites , Trypanosoma cruzi/classificação , Trypanosoma cruzi/genética , Animais , Bolívia/epidemiologia , Doença de Chagas/epidemiologia , DNA de Protozoário/genética , Variação Genética , Genética Populacional , Genótipo , Humanos , Epidemiologia Molecular , Peru/epidemiologia , FilogeniaRESUMO
Triatoma infestans is the main and most widespread vector of Chagas disease in South America. For the first time, a large sample of sylvatic populations of T. infestans was analyzed by ITS-2 and mtCytB sequencing. ITS-2 showed a low level of polymorphism but revealed a dichotomy between the Andean and non-Andean sylvatic populations. On the contrary, mtCytB sequences showed a high polymorphism (19 haplotypes determined by 35 variable sites) revealing a strong structuring between most of the sylvatic populations and possible ancient isolation and bottleneck in the Northern Andes. The dichotomy Andean vs. non-Andean populations was not observed with this marker. Moreover, mtCytB haplotype genealogies showed that the non-Andean haplotypes would have derived from the Andean ones, supporting somewhat an Andean origin of the species. Nevertheless, a non-Andean origin could not be discarded because a remarkable genetic diversity was found in the non-Andean sample. The comparison of the sylvatic haplotypes with the domestic ones from GenBank suggested multiple events of T. infestans domestication in Andean and non-Andean areas, instead of a major and unique domestication event in the Bolivian Andes, as previously proposed.