Microbiome and virome on indoor surfaces of an Antarctic research ship.

BACKGROUND: Few studies have focused on microbial diversity in indoor environments of ships, as well as the role of the microbiome and its ecological interconnections. In this study, we investigated the microbiome and virome present on the internal surfaces of a polar ship in different stages (beginning, during, and at the end) of the Brazilian Antarctic expedition in order to evaluate abundance of microorganisms in different periods. OBJECTIVES AND METHODS: We used shotgun metagenomic analysis on pooled samples from sampling surfaces in the ship's interior to track the microbial diversity. FINDINGS: Considering the total fraction of the microbiome, the relative abundance of bacteria, eukaryotes, viruses, and archaea was 83.7%, 16.2%, 0.04%, and 0.002%, respectively. Proteobacteria was the most abundant bacterial phyla, followed by Firmicutes, Actinobacteria, and Bacteroidetes. Concerning the virome, the greatest richness of viral species was identified during the middle of the trip, including ten viral families after de novo assembly: Autographiviridae, Chrysoviridae, Genomoviridae, Herelleviridae, Myoviridae, Partitiviridae, Podoviridae, Potyviridae, Siphoviridae, and Virgaviridae. MAIN CONCLUSIONS: This study contributed to the knowledge of microbial diversity in naval transportation facilities, and variations in the abundance of microorganisms probably occurred due to factors such as the number of passengers and activities on the ship.

Molecular Detection of Histoplasma capsulatum in Antarctica.

We detected Histoplasma capsulatum in soil and penguin excreta in the Antarctic Peninsula by sequencing after performing species-specific PCR, confirming previous observations that this pathogen occurs more broadly than suspected. This finding highlights the need for surveillance of emerging agents of systemic mycoses and their transmission among regions, animals, and humans in Antarctica.

Coxiella and Bartonella spp. in bats (Chiroptera) captured in the Brazilian Atlantic Forest biome.

BACKGROUND: The role of bats as reservoirs of zoonotic agents, especially pathogenic bacteria such as Bartonella and Coxiella, has been discussed around the world. Recent studies have identified bats as potential hosts of species from the proteobacteria phylum. In Brazil, however, the role of bats in the natural cycle of these agents is poorly investigated and generally neglected. In order to analyze the participation of bats in the epidemiology of diseases caused by Bartonella, Coxiella, Rickettsia, Anaplasma and Ehrlichia, we conducted a descriptive epidemiological study in three biogeographic regions of the Brazilian Atlantic Forest. RESULTS: Tissues of 119 bats captured in preserved areas in the states of Rio de Janeiro, Bahia and Santa Catarina from 2014 to 2015 were submitted to molecular analysis using specific primers. Bartonella spp. was detected in 22 spleen samples (18.5%, 95% CI: 11.9-26.6), whose phylogenetic analysis revealed the generation of at least two independent clusters, suggesting that these may be new unique genotypes of Bartonella species. In addition, four samples (3.4%, 95% CI: 0.9-8.3) were positive for the htpAB gene of C. burnetii [spleen (2), liver (1) and heart (1)]. Rickettsia spp., Anaplasma and Ehrlichia were not identified. This is the first study reporting C. burnetii and Bartonella spp. infections in bats from the Atlantic Forest biome. CONCLUSIONS: These findings shed light on potential host range for these bacteria, which are characterized as important zoonotic pathogens.

Virome analysis in lakes of the South Shetland Islands, Antarctica - 2020.

Polar freshwater ecosystems are characterized by a distinct microbiota. However, little is known about viral diversity and abundance, especially regarding the ecology of RNA viruses. We used shotgun metagenomic analysis on samples from Antarctic ecosystems, and report here the characterization of the virome fraction, from different lakes located in the South Shetland Islands (Penguin, Ardley, Deception and King George Island) in the Peninsula Antarctica, in the summer season 2020. DNA viruses (99.4 %) prevailed over RNA viruses (0.6 %) in the lake samples. Six viral orders were identified in the metagenomic libraries: Caudovirales (dsDNA), which was prevalent in most lakes; Picornavirales (ssRNA+); Sobelivirales (ssRNA+); Tolivirales (ssRNA+); Petitvirales (ssDNA) and Baphyvirales (ssDNA), including eight viral families (Herelleviridae, Siphoviridae, Myoviridae, Microviridae, Marnaviridae, Bacilladnaviridae, Barnaviridae and Tombusviridae) and several other, mainly non-classified ssRNA(+) viruses in the lakes of Ardley Island. Bacteriophages (dsDNA) (Herelleviridae family) infecting the phylum Firmicutes and Siphoviridae were predominant in most lakes evaluated. Functional analysis demonstrated a prevalence of unknown proteins (68 %) in the virome. Our prospective study provides virome analysis data from different lakes in the South Shetland Islands, Antarctica, opening exploratory lines for future research related to the biodiversity and viral ecology in this extreme ecosystem.

Microbiome and virome on indoor surfaces of an Antarctic research ship

BACKGROUND Few studies have focused on microbial diversity in indoor environments of ships, as well as the role of the microbiome and its ecological interconnections. In this study, we investigated the microbiome and virome present on the internal surfaces of a polar ship in different stages (beginning, during, and at the end) of the Brazilian Antarctic expedition in order to evaluate abundance of microorganisms in different periods. OBJECTIVES AND METHODS We used shotgun metagenomic analysis on pooled samples from sampling surfaces in the ship's interior to track the microbial diversity. FINDINGS Considering the total fraction of the microbiome, the relative abundance of bacteria, eukaryotes, viruses, and archaea was 83.7%, 16.2%, 0.04%, and 0.002%, respectively. Proteobacteria was the most abundant bacterial phyla, followed by Firmicutes, Actinobacteria, and Bacteroidetes. Concerning the virome, the greatest richness of viral species was identified during the middle of the trip, including ten viral families after de novo assembly: Autographiviridae, Chrysoviridae, Genomoviridae, Herelleviridae, Myoviridae, Partitiviridae, Podoviridae, Potyviridae, Siphoviridae, and Virgaviridae. MAIN CONCLUSIONS This study contributed to the knowledge of microbial diversity in naval transportation facilities, and variations in the abundance of microorganisms probably occurred due to factors such as the number of passengers and activities on the ship.

Molecular and morphological characterization of Contracaecum pelagicum (Nematoda) parasitizing Spheniscus magellanicus (Chordata) from Brazilian waters.

Three new sequences of Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2) from C. pelagicum parasite of Spheniscus magellanicus, the Magelanicus penguin, were determined from Brazilian waters. The sequences presented 99 and 98% of similarity with C. pelagicum sequences from Argentina, deposited on GenBank for the same genetic region and with a strong statistical support inferred from the phylogenetic tree. The morphological and ultrastructural studies that were carried out confirmed the genetic analysis.

[Diversity of wild and domestic mammal's intestinal helminths from the Caatinga of the Parque Nacional Serra da Capivara, Southeast of Piauí, Brazil]. / Diversidade de helmintos intestinais em mamíferos silvestres e domésticos na Caatinga do Parque Nacional Serra da Capivara, Sudeste do Piauí, Brasil.

Biodiversity studies allow ecosystem assessment and monitoring of environmental changes and impacts. Parasite diversity could reflect the host/ parasite coevolutionary process and the environment changes that permit the loss, gain or maintenance of species. This survey used species/morphotypes of helminths eggs found in feces from seven wild mammal species (the groups Dasypodidae and Large Cats, and Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu) and from two domestic species (Canis familiaris and Sus scrofa), which occur within the Serra da Capivara National Park (PNSC) and surrounding areas in order to analise the diversity of mammal intestinal helminths. This work used the helminthological fauna findings of wild and domestic mammals, to consider a possible helminth flux between these two host groups using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) of the hosts based on helminthological fauna composition. The results indicate that the region of the PNSC still maintains environmental conditions that still keep wild mammal helminthological fauna composition different from the one found for domestic mammals.

Molecular and morphological characterization of Contracaecum pelagicum (Nematoda) parasitizing Spheniscus magellanicus (Chordata) from Brazilian waters / Caracterização molecular e morfológica de Contracaecum pelagicum (Nematoda) parasito de Spheniscus magellanicus (Chordata) em águas brasileiras

Three new sequences of Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2) from C. pelagicum parasite of Spheniscus magellanicus, the Magelanicus penguin, were determined from Brazilian waters. The sequences presented 99 and 98% of similarity with C. pelagicum sequences from Argentina, deposited on GenBank for the same genetic region and with a strong statistical support inferred from the phylogenetic tree. The morphological and ultrastructural studies that were carried out confirmed the genetic analysis.

Foram determinadas três novas sequências da região do Citocromo c-oxidase da subunidade II do DNA mitocondrial (cox-2 mtDNA) de Contracaecum pelagicum, parasito de Spheniscus magellanicus, pinguim Magalhães, de águas brasileiras. As sequências apresentaram 99 e 98% de similaridade com sequências de C. pelagicum da Argentina depositadas no GenBank da mesma região genética com forte suporte estatístico inferido pela arvore filogenética. Estudos morfológicos e ultraestruturais realizados confirmaram a identidade genética.

Molecular and morphological characterization of Contracaecum pelagicum (Nematoda) parasitizing Spheniscus magellanicus (Chordata) from Brazilian waters

Foram determinadas três novas sequências da região do Citocromo c-oxidase da subunidade II do DNA mitocondrial (cox-2 mtDNA) de Contracaecum pelagicum, parasito de Spheniscus magellanicus, pinguim Magalhães, de águas brasileiras. As sequências apresentaram 99 e 98% de similaridade com sequências de C. pelagicum da Argentina depositadas no GenBank da mesma região genética com forte suporte estatístico inferido pela arvore filogenética. Estudos morfológicos e ultraestruturais realizados confirmaram a identidade genética.

Helmintos de mamíferos da região do Parque Nacional da Capivara, sudeste do Piauí: diversidade e influências antrópicas / Heminthes of mammals of the region of the National Park of the Capivara, southeast of the Piauí: diversity and influences anthropics

Este trabalho teve como objetivo contribuir para o conhecimento da fauna de helmintos intestinais dos mamíferos silvestres e domésticos que coabitam o Parque Nacional Serra da Capivara, no semi-árido do Piauí, e seu entorno. Este diagnóstico permite avaliar a possibilidade de emergência de patógenos nocivos ao homem e aos animais domésticos, que a cada dia estão mais próximos de ambientes naturais pela expansão das fronteiras agrícolas. (...) Estes dados contribuem para o conhecimento de formas imaturas de helmintos intestinais principalmente com a apresentação de fotos e amplitude de medidas dos ovos, média e desvio padrão de todas as medidas encontradas, documentado na forma de um apêndice. (AU)

Helmintos de mamíferos da região do Parque Nacional da Capivara, sudeste do Piauí: diversidade e influências antrópicas / Heminthes of mammals of the region of the National Park of the Capivara, southeast of the Piauí: diversity and influences anthropics

Este trabalho teve como objetivo contribuir para o conhecimento da fauna de helmintos intestinais dos mamíferos silvestres e domésticos que coabitam o Parque Nacional Serra da Capivara, no semi-árido do Piauí, e seu entorno. Este diagnóstico permite avaliar a possibilidade de emergência de patógenos nocivos ao homem e aos animais domésticos, que a cada dia estão mais próximos de ambientes naturais pela expansão das fronteiras agrícolas. Foram utilizadas fezes de sete espécies de mamíferos silvestres (os grupos Dasypodidae e Grandes felinos, e as espécies: Tamandua tetradactyla, Cebus apella, Alouatta caraya, Cerdocyon thous, Pecari tajacu) e duas espécies de mamíferos domésticos (Canis familiares e Sus scrofa). As amostras de fezes foram coletadas, entre outubro de 2004 a março de 2007, nas trilhas de trabalho no interior do PNSC, bem como nas estradas de acesso ao Parque em um raio de 10 km ao longo de seus limites. Estas fezes, depois de identificadas, foram analisadas pela técnica de sedimentação espontânea de Lutz (1919) para a busca de ovos (formas imaturas) de helmintos intestinais. Os ovos encontrados foram identificados no menor táxon possível, porém muitas vezes isso significou a identificação apenas da Superfamília ou Filo. Estes dados contribuem para o conhecimento de formas imaturas de helmintos intestinais principalmente com a apresentação de fotos e amplitude de medidas dos ovos, média e desvio padrão de todas as medidas encontradas, documentado na forma de um apêndice.

Helmintos de mamíferos da região do Parque Nacional da Capivara, sudeste do Piauí: diversidade e influências antrópicas / Heminthes of mammals of the region of the National Park of the Capivara, southeast of the Piauí: diversity and influences anthropics

Este trabalho teve como objetivo contribuir para o conhecimento da fauna de helmintos intestinais dos mamíferos silvestres e domésticos que coabitam o Parque Nacional Serra da Capivara, no semi-árido do Piauí, e seu entorno. Este diagnóstico permite avaliar a possibilidade de emergência de patógenos nocivos ao homem e aos animais domésticos, que a cada dia estão mais próximos de ambientes naturais pela expansão das fronteiras agrícolas. (...) Estes dados contribuem para o conhecimento de formas imaturas de helmintos intestinais principalmente com a apresentação de fotos e amplitude de medidas dos ovos, média e desvio padrão de todas as medidas encontradas, documentado na forma de um apêndice. (AU)