The Evaluation of a 5-miRNA Panel in Patients with Periodontitis Disease.

INTRODUCTION: Side by side with tooth decay, periodontitis remains one of the most common oral diseases and is increasingly recognized as a serious public health concern worldwide. OBJECTIVES: The present study aims at comparing the levels of 5 specific miRNAs (miR-29b-3p, miR-34a-5p, miR-155-5p, miR-181a-5p, and miR-192-5p) in patients with periodontal disease and healthy controls. METHODS: The pathogenic mechanism is related to the activation of immune response and significant alteration of coding and noncoding genes, including miRNA. The study includes 50 subjects (17 with periodontal disease and 33 healthy controls) with a mean age of 45.3 y. In both periodontitis patients and healthy controls, a panel of 5 miRNAs (miR-29b-3p, miR-34a-5p, miR-155-5p, miR-181a-5p, and miR-192-5p) is examined by determining their expression levels with quantitative reverse transcription polymerase chain reaction. RESULTS: The periodontitis patients express high levels of all the investigated miRNAs. Receiver operating characteristic curve analysis shows an area under the curve (AUC) of 0.69 to 0.74 for individual transcripts with the highest AUC value observed for miR-192, followed by miR-181a. CONCLUSIONS: The study indicates that the 5-miRNA panel can be used as biomarker for periodontitis. In this way, all implantology procedures and treatment options for patients diagnosed with periodontitis can be improved for better long-term results, predictability, and follow-up frequency. KNOWLEDGE TRANSFER STATEMENT: The discovery of a miRNA panel as a potential biomarker for periodontitis offers major opportunities for practical application. Our study can improve diagnostic accuracy; researchers can develop new theories on molecular mechanisms and biomarker discovery.

In Vivo Evaluation of Teeth Shade Match Capabilities of a Dental Intraoral Scanner.

Intraoral scanners were introduced in order to increase patient comfort and improve dentist lab communication. Acquiring optical impressions of the prepared teeth eliminates the need for conventional impression procedures and improves patient comfort. Intraoral scanner software offers since 2017, color shade determination, by analyzing the tooth shade of the obtained 3D model. In this study we tested the accuracy of an intraoral scanner color selection capabilities compared with a dental spectrophotometer, considered as reference. Statistical differences were found between the two system tested when the results were expressed in both Vita Classical and Vita 3D Master shade tabs codification.

Male infertility rate: a retrospective study.

INTRODUCTION: Worldwide, male responsible infertility is on the rise. In this retrospective study, we evaluated the infertility rate of this area. MATERIALS AND METHODS: Cases reported from 1989 to 1993 to the Institute for Post Graduate Teaching and Research in Ayurved University were studied. Semen reports were also scrutinized. RESULTS: A total number of 1,17,979 cases were examined. Male infertility rate was 45%. Patients' semen report showed that they were with oligozoospermia (22%), asthenozoospermia (11%) and azoospermia (12%). DISCUSSION: The reported value (45%) for male infertility is high, which is compared and discussed with that of authors from worldwide.