RESUMO
The chromosomal distribution of mobile genetic elements is scarcely known in Arvicolinae species, but could be of relevance to understand the origin and complex evolution of the sex chromosome heterochromatin. In this work we cloned two retrotransposon sequences, L1 and SINE-B1, from the genome of Chionomys nivalis and investigated their chromosomal distribution on several arvicoline species. Our results demonstrate first that both retroelements are the most abundant repeated DNA sequences in the genome of these species. L1 elements, in most species, are highly accumulated in the sex chromosomes compared to the autosomes. This favoured L1 insertion could have played an important role in the origin of the enlarged heterochromatic blocks existing in the sex chromosomes of some Microtus species. Also, we propose that L1 accumulation on the X heterochromatin could have been the consequence of different, independent and rapid amplification processes acting in each species. SINE elements, however, were completely lacking from the constitutive heterochromatin, either in autosomes or in the heterochromatic blocks of sex chromosomes. These data could indicate that some SINE elements are incompatible with the formation of heterochromatic complexes and hence are necessarily missing from the constitutive heterochromatin.
Assuntos
Arvicolinae/genética , Genoma , Heterocromatina/genética , Elementos Nucleotídeos Longos e Dispersos/genética , Retroelementos/genética , Elementos Nucleotídeos Curtos e Dispersos/genética , Animais , Feminino , Hibridização in Situ Fluorescente , Masculino , Dados de Sequência Molecular , Análise de Sequência de DNA , Cromossomos Sexuais/genéticaRESUMO
SOX proteins form a large family of transcription factors related by a DNA-binding domain known as the HMG box. Some 30 Sox genes have been identified in mammals and orthologues have been found in a wide range of other metazoans. Sox genes are highly conserved and are known to play important roles in embryonic development, including roles in gonadal, central nervous system, neural crest and skeletal development. Several SOX genes have been implicated in human congenital diseases. We report here the isolation of Sox8 and its characterisation in mice and humans. This gene has a remarkably similar primary structure and genomic organisation to the campomelic dysplasia gene SOX9 and the Waardenburg-Shah syndrome gene SOX10. SOX8 protein is able to bind to canonical SOX target DNA sequences and activate transcription in vitro through two separate trans -activation regions. Further, Sox8 is expressed in the central nervous system, limbs, kidneys, gonads and craniofacial structures during mouse embryo development. Sox8 maps to the t complex on mouse chromosome 17 and to human chromosome 16p13.3, a region associated with the microphthalmia-cataract syndrome CATM and the alpha-thalassemia/mental retardation syndrome ATR-16.
Assuntos
Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Proteínas Nucleares , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Sequência de Aminoácidos , Animais , Cromossomos Humanos Par 16/genética , Clonagem Molecular , DNA/genética , DNA/metabolismo , Embrião de Mamíferos/metabolismo , Éxons/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas de Grupo de Alta Mobilidade/química , Proteínas de Grupo de Alta Mobilidade/genética , Humanos , Íntrons/genética , Camundongos , Dados de Sequência Molecular , Mapeamento Físico do Cromossomo , RNA Mensageiro/análise , RNA Mensageiro/genética , Elementos de Resposta/genética , Fatores de Transcrição SOX9 , Fatores de Transcrição SOXE , Alinhamento de Sequência , Proteína da Região Y Determinante do Sexo , Síndrome , Fatores de Transcrição/química , Ativação Transcricional/genéticaRESUMO
The Arvicolidae is a widely distributed rodent group with several interesting characteristics in their sex chromosomes. Here, we summarize the actual knowledge of some of these characteristics. This mammalian group has species with abnormal sex determination systems. In fact, some species present the same karyotype in both males and females, with total absence of a Y chromosome, and hence of SRY and ZFY genes. Other species present fertile, sex-reversed XY females, generally due to mutations affecting X chromosomes. Furthermore, in Microtus oregoni males and females are gonosomic mosaic (the females are XO in the soma and XX in the germ cells, while the males are XY in the soma and OY in the germ cells). Regarding sex chromosomes, some species present enlarged (giant) sex chromosomes because of the presence of large blocks of constitutive heterochromatin, which have been demonstrated to be highly heterogeneous. Furthermore, we also consider the alterations affecting composition and localization of sex-linked genes or repeated sequences. Finally, this rodent group includes species with synaptic and asynaptic sex chromosomes. In fact, several species with asynaptic sex chromosomes have been described. It is interesting to note that within the genus Microtus both types of sex chromosomes are present.
Assuntos
Arvicolinae/genética , Cromossomos Sexuais , Processos de Determinação Sexual , Animais , Sequência de Bases , Mapeamento Cromossômico , Pareamento Cromossômico , Elementos de DNA Transponíveis , Proteínas de Ligação a DNA/genética , Feminino , Masculino , Sequências Repetitivas de Ácido Nucleico , Cromossomos Sexuais/química , Cromossomos Sexuais/ultraestrutura , Cromossomo X , Cromossomo YRESUMO
The satellite DNA Msat-160 has been previously characterized in several species of the genus Microtus. Here we present the characterization of Msat-160 from Chionomys nivalis, a species with a very primitive karyotype. As in other Microtus species analyzed, C. nivalis Msat-160 is AT rich, has a monomer length of 160 bp, is undermethylated and is mainly located in all the pericentromeric heterochromatin of all autosomes and the X chromosome, but is completely absent from the Y chromosome. Hence, our results support the hypothesis that Msat-160 was initially distributed in the pericentromeric heterochromatin of all autosomes and the X chromosome. The taxonomic status of the genus Chionomys in relation to the genus Microtus is a very interesting issue, so we constructed phylogenetic dendrograms using Msat-160 sequences from several Microtus species. Although the results were not informative about this issue, the presence of Msat-160 in C. nivalis and Microtus species suggested that both genera are closely related and that this satellite DNA was present in the common ancestor. Studies of Msat-160 in different arvicoline species could help to determine the origin of this satellite and, perhaps, to establish the phylogenetic relationships of some arvicoline groups.
Assuntos
Arvicolinae/genética , DNA Satélite/análise , Animais , Sequência de Bases , Feminino , Hibridização in Situ Fluorescente , Masculino , Dados de Sequência Molecular , Filogenia , Mapeamento por Restrição , Homologia de Sequência do Ácido NucleicoRESUMO
Long interspersed nuclear elements (L1 or LINE-1) are the most abundant and active retroposons in the mammalian genome. Traditionally, the bulk of L1 sequences have been explained by the 'selfish DNA' hypothesis; however, recently it has been also argued that L1s could play an important role in genome and gene organizations. The non-random chromosomal distribution of these retroelements is a striking feature considered to reflect this functionality. In the present study we have cloned and analyzed three different L1 fragments from the genome of the rodent Microtus cabrerae. In addition, we have examined the chromosomal distribution of this L1 in several species of Microtus, a very interesting group owing to the presence in some species of enlarged ('giant') sex chromosomes. Interestingly, in all species analyzed, L1-retroposons have preferentially accumulated on both the giant- and the normal-sized sex chromosomes compared with the autosomes. Also we have demonstrated that L1-retroposons are not similarly distributed among the heterochromatic blocks of the giant sex chromosomes in M. cabrerae and M. agrestis, which suggest that L1 retroposition and amplification over the sex heterochromatin have been different and independent processes in each species. Finally, we proposed that the main factors responsible for the L1 distribution on the mammalian sex chromosomes are the heterochromatic nature of the Y chromosome and the possible role of L1 sequences during the X-inactivation process.
Assuntos
Arvicolinae/genética , Evolução Molecular , Elementos Nucleotídeos Longos e Dispersos , Retroelementos , Cromossomos Sexuais , Animais , Clonagem Molecular , Feminino , Heterocromatina/genética , Hibridização in Situ Fluorescente , Elementos Nucleotídeos Longos e Dispersos/genética , Masculino , Metilação , Modelos Genéticos , Retroelementos/genética , Cromossomos Sexuais/genética , Cromossomos Sexuais/metabolismo , Especificidade da EspécieRESUMO
We have studied the spatial dynamics of Sry transcription in the genital ridges of mouse embryos. We find that Sry is expressed in a dynamic wave that emanates from the central and/or anterior regions, extends subsequently to both poles, and ends in the caudal pole. This dynamism may explain the relative positioning of ovarian and testicular tissue seen in ovotestes in mice. Since direct regulatory targets of SRY ought to be expressed in a corresponding or complimentary wave, our observations pave the way for identification of target genes. Sry is expressed in internal cells but not in coelomic surface epithelial cells, indicating that its effect on proliferation of surface cells is achieved non-cell-autonomously. The cellular dynamism of Sry expression revealed in this study thus provides important insights into both the cellular and molecular mode of action of SRY, and how perturbations in Sry expression can lead to anomalies of sexual development.
Assuntos
Proteínas de Ligação a DNA/biossíntese , Genitália/embriologia , Proteínas Nucleares , Fatores de Transcrição , Animais , Divisão Celular , Células Epiteliais/metabolismo , Ligação Genética , Proteínas de Fluorescência Verde , Hibridização In Situ , Proteínas Luminescentes/metabolismo , Camundongos , Proteína da Região Y Determinante do Sexo , Fatores de Tempo , Distribuição Tecidual , Cromossomo XRESUMO
Sry, a gene from the Y chromosome, is known to initiate testis formation and subsequent male differentiation in mammals. A related gene, Sox9, also plays a critical role in testis determination, possibly in all vertebrates. A number of models have been presented regarding the molecular modes of action of these two genes. However, details regarding their regulation, regulatory target genes, and interacting protein factors and co-factors have not been established with any certainty. In this review, we examine new evidence and re-examine existing evidence bearing on these issues, in an effort to build up an integrative model of the network of gene activity centred around Sry and Sox9. J. Exp. Zool. 290:463-474, 2001.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas de Grupo de Alta Mobilidade/genética , Proteínas Nucleares , Proteínas Repressoras , Caracteres Sexuais , Diferenciação Sexual/genética , Fatores de Transcrição/genética , Animais , Receptor Nuclear Órfão DAX-1 , Proteínas de Ligação a DNA/fisiologia , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Proteínas de Grupo de Alta Mobilidade/fisiologia , Humanos , Masculino , Camundongos , Modelos Biológicos , Receptores do Ácido Retinoico/genética , Receptores do Ácido Retinoico/fisiologia , Sequências Reguladoras de Ácido Nucleico , Fatores de Transcrição SOX9 , Processos de Determinação Sexual , Proteína da Região Y Determinante do Sexo , Testículo/embriologia , Fatores de Transcrição/fisiologiaRESUMO
Little is known of the mechanisms whereby the mammalian indifferent gonad develops into a testis or ovary. In XY individuals, Sry, the mammalian testis-determining gene, is expressed in the pre-Sertoli cells, which then differentiate into Sertoli cells. Other cell types, which include the germ cells, the steroidogenic cells and the connective tissue cells, must then be instructed to develop in a male-specific manner. Although some genes involved in sex-determination and differentiation processes have been identified, we know little of how they interact and cooperate to orchestrate the development of a testis or ovary. We have initiated an expression-screening program designed to identify additional genes, known or novel, which play a role in these processes. This approach is based on our belief that many of the genes we seek will be expressed in a sex-specific manner during the period of sex-determination and differentiation. Most of the genes identified previously are transcription factors and so we aim, in particular, to find genes involved in cell-to-cell communication, signal transduction, and transcriptional regulation, downstream of the differentiation of Sertoli cells. We have used a suppression subtractive-hybridization method to generate male- and female-enriched probes and libraries. Clones are validated as being sex-specific in their expression patterns by array screening and in situ hybridization. Here we report on our progress to date and the general applicability of the approach for studies in other systems. J. Exp. Zool. 290:517-522, 2001.
Assuntos
Gônadas/embriologia , Processos de Determinação Sexual , Diferenciação Sexual/genética , Animais , Feminino , Expressão Gênica , Biblioteca Gênica , Masculino , Mamíferos , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase/métodos , Diferenciação Sexual/fisiologiaRESUMO
The molecular pathways leading from indifferent mammalian gonad to either testis or ovary are not well understood. A number of genes, including the Y-linked sex determining gene SRY, have been shown to play roles in sex determination or differentiation, but there are clearly many missing elements to be found. We used suppression-subtractive hybridization to construct normalized cDNA libraries enriched for male-specific or female-specific transcripts in mouse fetal gonads. We describe the strategy used to efficiently screen these libraries for candidate sex-determination and gonadogenesis genes. One gene arising from these screens is vanin-1, which encodes a protein implicated in the induction of cell migration into the thymus. We find that vanin-1 is expressed male-specifically in Sertoli cells of the developing testis and may be involved in inducing cell migration from the adjacent mesonephros, a process known to be critical for testis development. This screening approach is likely to be applicable to the isolation and study of genes involved in a variety of developmental systems.
Assuntos
Moléculas de Adesão Celular/genética , Regulação da Expressão Gênica no Desenvolvimento , Testículo/embriologia , Amidoidrolases , Animais , Moléculas de Adesão Celular/fisiologia , DNA Complementar , Proteínas de Ligação a DNA/genética , Feminino , Proteínas Ligadas por GPI , Biblioteca Gênica , Hibridização In Situ , Masculino , Camundongos , Fator 3 de Transcrição de Octâmero , Processos de Determinação Sexual , Fatores de Transcrição/genéticaRESUMO
Cytogenetic studies of Erinaceus europaeus from a Spanish population, including C-banding, simultaneous Ag-staining of nucleolus organizer regions (NORs) and G-banding, and chromomycin A3 staining, are described. With regard to the number and morphology of chromosomes, Spanish hedgehogs showed a karyotype very similar to that described by Mandal for Northern Europe specimens, referred to as the WII karyotype. Clear differences were observed in the presence or absence of centromeric C-bands and the number and position of NORs. A new karyotypic form, provisionally designated the S (south) karyotype, is proposed.
Assuntos
Variação Genética/genética , Ouriços/genética , Cariotipagem , Animais , Cromomicina A3 , Bandeamento Cromossômico , Região Organizadora do Nucléolo , Coloração pela Prata , EspanhaRESUMO
SOX genes are a family of genes that encode for proteins which are characterised by the presence of a HMG-domain related to that of the mammalian sex-determining gene (SRY). By definition, the DNA binding domain of SOX genes is at least 50% identical to the 79 amino acid HMG domain of the SRY gene. We report here two HMG-box sequences from two microbat species (R. ferrumequinum and P. Pipistrellus) which were PCR amplified using a primer pair specific to the mouse Sry HMG-box. The high percentage of identity of this sequences with the human and mouse SOX30 HMG-box suggests that they are the SOX30 HMG-box for these two bat species.
Assuntos
Quirópteros/genética , Proteínas de Ligação a DNA/genética , Domínios HMG-Box/genética , Proteínas de Grupo de Alta Mobilidade/genética , Proteínas de Peixe-Zebra , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA/genética , Primers do DNA/genética , Feminino , Genes sry , Humanos , Masculino , Camundongos , Dados de Sequência Molecular , Fatores de Transcrição SOXB2 , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
Sex determination in mammals is controlled by the Y-linked SRY gene, which encodes a transcription factor with a DNA-binding motif of the HMG type. The only conserved region in this gene is the HMG-box, whose nucleotide sequence is currently available in a number of mammalian taxa. However, nothing is known about this gene in bats. Here, we report partial sequences of the SRY HMG-box from four microbat and four megabat species. We used the SRY HMG- box sequences from micro- and megabats to test the phylogenetic relationships between microbats, megabats, and primates. In maximum parsimony and maximum-likelihood trees, mega- and microbat branches start in the same internal node, which is consistent with a monophyletic origin of this mammalian group.
Assuntos
Quirópteros/genética , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/genética , Proteínas de Grupo de Alta Mobilidade/química , Proteínas Nucleares , Filogenia , Fatores de Transcrição , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Feminino , Humanos , Funções Verossimilhança , Masculino , Camundongos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Primatas/genética , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Proteína da Região Y Determinante do Sexo , Cromossomo Y/genéticaRESUMO
Sex determination in mammals is controlled by SRY (sex-determining region of the Y chromosome), a single-copy gene located on the Y-specific region. Several exceptions to this rule have been described: some rodent species present Y-specific multiple copies (either mono- or polymorphic) of this gene, and two Ellobius species and one Tokudaia species determine sex without a Y chromosome or the SRY gene. Recently, we have described multiple polymorphic copies of the SRY gene in both males and females of the vole species Microtus cabrerae. The female location and the presence of stop codons in some copies from males and females also suggest that they are nonfunctional copies of this gene (pseudogenes). We have investigated the SRY HMG-box in nine species of the family Microtidae; we report here the presence, in eight of these species, of multiple mono- or polymorphic copies of the SRY gene located on the Y chromosome.
Assuntos
Arvicolinae/genética , Proteínas de Ligação a DNA/genética , Dosagem de Genes , Proteínas Nucleares , Mapeamento Físico do Cromossomo , Polimorfismo Genético/genética , Fatores de Transcrição , Cromossomo Y/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , Animais , Arvicolinae/classificação , Sequência de Bases , Southern Blotting , Códon de Terminação/genética , Proteínas de Ligação a DNA/química , Feminino , Amplificação de Genes/genética , Masculino , Dados de Sequência Molecular , Mutação , Reação em Cadeia da Polimerase , Pseudogenes/genética , Alinhamento de Sequência , Proteína da Região Y Determinante do SexoRESUMO
We studied the anatomical, histological, and genetic features of the sexual tract in four European mole species of the genus Talpa (Insectivora, mammalia): T. occidentalis, T. europaea, T. romana, and T. stankovici. All XY individuals had a normal male phenotype, whereas all XX individuals in all four species had features that identified them as intersexes. These individuals were nonetheless presumed to be functionally fertile females. Intersexuality was manifested mainly as gonadal hermaphroditism, with all females possessing bilateral ovotestes. The gonads were composed of a small portion of histologically normal ovarian tissue and a variably sized, generally large mass of disgenetic testicular tissue, accompanied by a small, rudimentary epididymis. The rest of the sexual tract was typically female, including oviducts, uterus, and vagina of normal appearance. Polymerase chain reaction (PCR) and Southern blotting analyses showed that the mammalian testis-determining gene SRY is present in males but not in females. Part of the conserved sequence of the mole SRY gene was cloned and sequenced after PCR amplification in two of the four mole species (T. occidentalis from Spain and T. romana from Italy). Sequences were identical in these two species and were very similar to those of the human and mouse SRY gene. Our findings constitute the first evidence of the existence of a genus-specific case of true hermaphroditism, probably due to a very ancient mutation that fixed in populations of the ancestral species from which contemporary moles evolved. The possible nature of this mutation is discussed with regard to the cytologic, histologic, and genetic features of the gonads in Talpa females.
Assuntos
Transtornos do Desenvolvimento Sexual/genética , Toupeiras/genética , Animais , Sequência de Bases , Feminino , Humanos , Masculino , Camundongos , Biologia Molecular , Dados de Sequência Molecular , Toupeiras/anatomia & histologia , Homologia de Sequência do Ácido NucleicoRESUMO
In mammals, sex determination is controlled by the Y-linked gene SRY. Although SRY is male-specific in most eutherian and marsupial species, with a single copy on the Y chromosome, several rodent species have multiple Y-linked copies of SRY, and two mole-vole species of the genus Ellobius determine sex without the Y chromosome or the SRY gene. We searched for homologs of SRY in three vole species of the genus Microtus and concluded that this gene is not male-specific in M. cabrerae, as it is present in multiple, polymorphic copies in both males and females. In contrast, SRY is male-specific in the related species M. agrestis and M. nivalis. Up to 15 different partial sequences of the SRY gene were found in M. cabrerae. Southern blots suggest that most of the extra copies of SRY are X-linked. One of the copies observed only in males has a sequence identical to that of the SRY gene in M. agrestis and may represent a functional copy of the gene in this species. The rest are probably nonfunctional pseudogenes.
Assuntos
Arvicolinae/genética , Proteínas de Ligação a DNA/genética , Proteínas Nucleares , Fatores de Transcrição , Animais , Sequência de Bases , Feminino , Humanos , Masculino , Camundongos , Dados de Sequência Molecular , Sequências Repetitivas de Ácido Nucleico , Alinhamento de Sequência , Proteína da Região Y Determinante do SexoRESUMO
Repetitive DNA sequences represent a substantial component of eukaryotic genomes. These sequences have been described and characterized in many mammalian species. However, little information about repetitive DNA sequences is available in bat species. Here we describe an EcoRI family of repetitive DNA sequences present in the species Miniopterus schreibersi. These repetitive sequences are 57.85%, A-T rich, organized in tandem, and with a monomer unit length of 904 bp. Methylation analysis using the isoesquizomer pair MspI and HpaII indicates that the cytosines present in the sequences CCGG are partially methylated. Furthermore, Southern blot analysis demonstrated that these DNA sequences are absent in the genomes of four related microbat species and suggest that it could be specific to the M. schreibersi genome.