RESUMO
Direct product sequestration of extracellular proteins from microbial batch cultures can be achieved by continuous or intermittent broth recycle through an external extractive loop. Here, we describe the development of a fluidisable, mixed mode adsorbent, designed to tolerate increasing ionic strength (synonymous with extended productive batch cultures). This facilitated operations for the integrated recovery of an extracellular acid protease from cultures of Yarrowia lipolytica. Mixed mode adsorbents were prepared using chemistries containing hydrophobic and ionic groups. Matrix hydrophobicity and titration ranges were matched to the requirements of integrated protease adsorption. A single expanded bed was able to service the productive phase of growth without recourse to the pH adjustment of the broth previously required for ion exchange adsorption. This resulted in increased yields of product, accompanied by further increases in enzyme specific activity. A step change from pH 4.5 to 2.6, across the isoelectric point of the protease, enabled high resolution fixed bed elution induced by electrostatic repulsion. The generic application of mixed mode chemistries, which combine the physical robustness of ion-exchange ligands in sanitisation and sterilisation procedures with a selectivity, which approaches that of affinity interactions, is discussed.
Assuntos
Ácido Aspártico Endopeptidases/isolamento & purificação , Proteínas Fúngicas , Leveduras/enzimologia , Adsorção , Ácido Aspártico Endopeptidases/metabolismo , Cromatografia/métodos , Eletroforese em Gel de Poliacrilamida , Fermentação , Concentração de Íons de HidrogênioRESUMO
A new form of protein chromatography, hydrophobic charge induction, is described. Matrices prepared by attachment of weak acid and base ligands were uncharged at absorption pH. At low ligand densities, protein adsorption was typically promoted with lyotropic salts. At higher ligand densities, chymosin, chymotrypsinogen and lysozyme were adsorbed independently of ionic strength. A pH change released the electrostatic potential of the matrix and weakened hydrophobic interactions, inducing elution. Matrix hydrophobicity and titration range could be matched to protein requirements by ligand choice and density. Both adsorption and elution could be carried out within the pH 5-9 range.
Assuntos
Proteínas/isolamento & purificação , Adsorção , Aminas/química , Soluções Tampão , Ácidos Carboxílicos/química , Fenômenos Químicos , Físico-Química , Cromatografia , Quimosina/química , Quimosina/isolamento & purificação , Concentração de Íons de Hidrogênio , Ligantes , Proteínas/química , Reagentes de SulfidrilaRESUMO
The role of chromatography in capture is reviewed in terms of the special requirements imposed by the processing of very crude feedstocks. Adsorption methods which are not significantly affected by variations of feedstock ionic strength are highlighted. Methods are compared in terms of simplicity, robustness, selectivity and ease of elution. The application of such methods to enzyme and antibody purifications is summarised. Particular emphasis is placed on high ligand density methods, which have potential for broad-spectrum application.
Assuntos
Cromatografia de Afinidade/métodos , Proteínas/isolamento & purificação , Adsorção , Anticorpos/isolamento & purificação , Enzimas/isolamento & purificação , Ligantes , Reprodutibilidade dos Testes , Sais , Sensibilidade e EspecificidadeRESUMO
In a study with 15 neonatal foals (5 per treatment group), foals were fed within 4 hours of birth as follows: 250 ml of colostrum, 250 ml of lyophilized serum reconstituted at 5 times the original concentration, or 250 ml of a mixture (1:1) of colostrum and lyophilized serum. Foal serum samples were tested for immunoglobulin (Ig)G concentration and titrated for anti-equine rhinovirus 1 and anti-equine influenza A1 and A2 antibodies at 0 and 24 hours after foals were born. Except in a foal which had suckled the dam before treatment, there was no evidence of IgG or specific viral antibodies in the samples taken at birth. There were no significant differences found in the serum IgG concentrations and antibody titers among the 3 treatment groups. Seemingly, IgG was absorbed efficiently from both serum and colostrum, so that the use of reconstituted lyophilized serum as a prophylactic measure of conferring passive immunity to a newborn foal deserves serious consideration.
Assuntos
Animais Recém-Nascidos/imunologia , Anticorpos Antivirais/análise , Cavalos/imunologia , Soros Imunes/imunologia , Administração Oral , Animais , Colostro/imunologia , Liofilização , Soros Imunes/administração & dosagem , Imunoglobulina G/análise , Orthomyxoviridae/imunologia , Picornaviridae/imunologiaRESUMO
Although the advent of atypical, second-generation antipsychotics (SGAs) has resulted in reduced likelihood of akathisia, this adverse effect remains a problem. It is known that extrapyramidal adverse effects are associated with increased drug occupancy of the dopamine 2 receptors (DRD2). The A1 allele of the DRD2/ANKK1, rs1800497, is associated with decreased striatal DRD2 density. The aim of this study was to identify whether the A1(T) allele of DRD2/ANKK1 was associated with akathisia (as measured by Barnes Akathisia Rating Scale) in a clinical sample of 234 patients who were treated with antipsychotic drugs. Definite akathisia (a score ≥ 2 in the global clinical assessment of akathisia) was significantly less common in subjects who were prescribed SGAs (16.8%) than those prescribed FGAs (47.6%), p < 0.0001. Overall, 24.1% of A1+ patients (A1A2/A1A1) who were treated with SGAs had akathisia, compared to 10.8% of A1- (thus, A2A2) patients. A1+ patients who were administered SGAs also had higher global clinical assessment of akathisia scores than the A1- subjects (p = 0.01). SGAs maintained their advantage over FGAs regarding akathisia, even in A1+ patients who were treated with SGAs. These results strongly suggested that A1+ variants of the DRD2/ANKK1 Taq1A allele do confer an associated risk for akathisia in patients who were treated with SGAs, and these variants may explain inconsistencies found across prior studies, when comparing FGAs and SGAs.
Assuntos
Acatisia Induzida por Medicamentos/genética , Antipsicóticos/efeitos adversos , Polimorfismo de Nucleotídeo Único , Proteínas Serina-Treonina Quinases/genética , Receptores de Dopamina D2/genética , Esquizofrenia/tratamento farmacológico , Antagonistas do Receptor 5-HT2 de Serotonina/efeitos adversos , Adulto , Acatisia Induzida por Medicamentos/epidemiologia , Acatisia Induzida por Medicamentos/metabolismo , Antidepressivos de Segunda Geração/efeitos adversos , Antidepressivos de Segunda Geração/uso terapêutico , Antipsicóticos/uso terapêutico , Centros Comunitários de Saúde Mental , Estudos Transversais , Manual Diagnóstico e Estatístico de Transtornos Mentais , Antagonistas dos Receptores de Dopamina D2 , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Hospitais de Ensino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Proteínas Serina-Treonina Quinases/metabolismo , Queensland/epidemiologia , Receptores de Dopamina D2/metabolismo , Antagonistas do Receptor 5-HT2 de Serotonina/uso terapêuticoRESUMO
Mixed mode Sepharose and Perloza bead cellulose matrices were prepared using various chemistries. These matrices contained hydrophobic (aliphatic and/or aromatic) and ionic (carboxylate or alkylamine) groups. Hydrophobic amine ligands were attached to epichlorohydrin activated Sepharose (mixed mode amine matrices). Hexylamine, aminophenylpropanediol and phenylethylamine were the preferred ligands, on the basis of cost and performance. Other mixed mode matrices were produced by incomplete attachment (0-80%) of the same amine ligands to carboxylate matrices. The best results were obtained using unmodified or partially ligand-modified aminocaproic acid Sepharose and Perloza. High ligand densities were used, resulting in high capacity. Furthermore, chymosin was adsorbed at high and low ionic strengths, which reduced sample preparation requirements. Chymosin, essentially homogeneous by electrophoresis, was recovered by a small pH change. The methods described were simple, efficient, inexpensive and provided very good resolution of chymosin from a crude recombinant source. The carboxylate matrices had the best combination of capacity and regeneration properties. The performance of Sepharose and Perloza carboxylate matrices was similar, but higher capacities were found for the latter. Because it is cheaper and can be used at higher flow rates, Perloza should be better suited to large scale application. High capacity chymosin adsorption was found with carboxymethyl ion exchange matrices, but low ionic strength was essential for adsorption and the purity was inferior to that of the mixed mode matrices.