A Testis-Specific DMRT1 (Double Sex and Mab-3-Related Transcription Factor 1) Plays a Role in Spermatogenesis and Gonadal Development in the Hermaphrodite Boring Giant Clam Tridacna crocea.

The testis-specific double sex and mab-3-related transcription factor 1 (DMRT1) has long been recognized as a crucial player in sex determination across vertebrates, and its essential role in gonadal development and the regulation of spermatogenesis is well established. Here, we report the cloning of the key spermatogenesis-related DMRT1 cDNA, named Tc-DMRT1, from the gonads of Tridacna crocea (T. crocea), with a molecular weight of 41.93 kDa and an isoelectric point of 7.83 (pI). Our hypothesis is that DMRT1 machinery governs spermatogenesis and regulates gonadogenesis. RNAi-mediated Tc-DMRT1 knockdown revealed its critical role in hindering spermatogenesis and reducing expression levels in boring giant clams. A histological analysis showed structural changes, with normal sperm cell counts in the control group (ds-EGFP) but significantly lower concentrations of sperm cells in the experimental group (ds-DMRT1). DMRT1 transcripts during embryogenesis exhibited a significantly high expression pattern (p < 0.05) during the early zygote stage, and whole-embryo in-situ hybridization confirmed its expression pattern throughout embryogenesis. A qRT-PCR analysis of various reproductive stages revealed an abundant expression of Tc-DMRT1 in the gonads during the male reproductive stage. In-situ hybridization showed tissue-specific expression of DMRT1, with a positive signal detected in male-stage gonadal tissues comprising sperm cells, while no signal was detected in other stages. Our study findings provide an initial understanding of the DMRT1 molecular machinery controlling spermatogenesis and its specificity in male-stage gonads of the key bivalve species, Tridacna crocea, and suggest that DMRT1 predominantly functions as a key regulator of spermatogenesis in giant clams.

A Review on Automatic Facial Expression Recognition Systems Assisted by Multimodal Sensor Data.

Facial Expression Recognition (FER) can be widely applied to various research areas, such as mental diseases diagnosis and human social/physiological interaction detection. With the emerging advanced technologies in hardware and sensors, FER systems have been developed to support real-world application scenes, instead of laboratory environments. Although the laboratory-controlled FER systems achieve very high accuracy, around 97%, the technical transferring from the laboratory to real-world applications faces a great barrier of very low accuracy, approximately 50%. In this survey, we comprehensively discuss three significant challenges in the unconstrained real-world environments, such as illumination variation, head pose, and subject-dependence, which may not be resolved by only analysing images/videos in the FER system. We focus on those sensors that may provide extra information and help the FER systems to detect emotion in both static images and video sequences. We introduce three categories of sensors that may help improve the accuracy and reliability of an expression recognition system by tackling the challenges mentioned above in pure image/video processing. The first group is detailed-face sensors, which detect a small dynamic change of a face component, such as eye-trackers, which may help differentiate the background noise and the feature of faces. The second is non-visual sensors, such as audio, depth, and EEG sensors, which provide extra information in addition to visual dimension and improve the recognition reliability for example in illumination variation and position shift situation. The last is target-focused sensors, such as infrared thermal sensors, which can facilitate the FER systems to filter useless visual contents and may help resist illumination variation. Also, we discuss the methods of fusing different inputs obtained from multimodal sensors in an emotion system. We comparatively review the most prominent multimodal emotional expression recognition approaches and point out their advantages and limitations. We briefly introduce the benchmark data sets related to FER systems for each category of sensors and extend our survey to the open challenges and issues. Meanwhile, we design a framework of an expression recognition system, which uses multimodal sensor data (provided by the three categories of sensors) to provide complete information about emotions to assist the pure face image/video analysis. We theoretically analyse the feasibility and achievability of our new expression recognition system, especially for the use in the wild environment, and point out the future directions to design an efficient, emotional expression recognition system.