[Primary culture and identification of mouse renal proximal tubular epithelial cells].

The present study was aimed to establish a modified method for culturing mouse renal proximal tubular epithelial cells (TECs). Renal cortex was isolated from mouse kidney and scissored into pieces. TECs were separated by digesting scissored renal cortex in type II collagenase combined with strainer filtration, and then cultured in DMEM. The morphology of TECs was observed under inverted microscopy. The cell proliferative ability was assessed by flow cytometry, and cell viability was analyzed by CCK-8 assay. The purity of TECs was identified by immunofluorescence. Immunofluorescence observation showed that more than 95% cells were epithelial marker CK18 positive and more than 90% cells expressed renal proximal TECs marker proteins, Villin, AQP1, and SGLT2. The cells could be subcultured for about 5 times. The cell proliferative ability declined following the repeated passage. This study introduced a modified efficient method for culturing highly purified mouse renal proximal TECs.

[Regional difference of land use/cover change in farming-pasturing zone of Naiman Banner in Inner Mongolia].

Based on the four TM images of Naiman Banner in Inner Mongolia in 1975, 1985, 1995 and 2005, the extent and relative rate of land use change were used as the indices to analyze the regional difference of land use/cover change in the farming-pasturing zone of Naiman Banner, and the indices abundance and importance value were adopted to analyze the spatial distribution features of land use/cover change in the study area. The results showed that from 1975 to 2005, the types of land use/cover became diversified. The annual change rate was high, and the regional difference was significant. In the northern alluvial plain sub-area, woodland area increased rapidly and largely, while sandy land area decreased obviously. The changing speed of the areas of meadow and sandy land was the fastest, while that of residential area was the slowest. The main forms of land conversion were the conversion from sandy land to cropland and woodland. In the middle sandy land sub-area, sandy land had a wide distribution. The changes of other land use types were comparatively small, and the main form of land conversion was the inter-conversion between cropland and sandy land. In southern loess sub-area, cropland was the dominant land use type and had the smallest change, meadow and sandy land changed most quickly, and the conversion from meadow to cropland and woodland was the most important land conversion form. Natural factors determined the principal characteristics of land use structure in each sub-area of the study area, and artificial factors determined the changing trends of each land use type in each sub-area.