Pneumocystis jirovecii among patients with cystic fibrosis and their household members.

We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection. LAY SUMMARY: This study suggests for the first time the possible transmission of Pneumocystis in the family environment of patients with cystic fibrosis, indicating that patients and their household members are reservoirs and possible sources of this infection.

Mass animal sacrifice at casas del Turuñuelo (Guareña, Spain): A unique Tartessian (Iron Age) site in the southwest of the Iberian Peninsula.

Zooarchaeological analyses of the skeletal remains of 52 animals unearthed in the courtyard of an Iron Age Tartessian building known as Casas del Turuñuelo (Badajoz, Spain) shed light on a massive sacrifice forming part of a series of rituals linked to the site's last period of activity and final abandonment. The rites took place towards the end of the 5th century BCE when both the building (intentionally destroyed) and the sacrificed animals were intentionally buried under a tumulus 90 m in diameter and 6 m high. The main objective of the zooarchaeological and microstratigraphic analyses was to determine the phasing of the sacrificial depositions. Evidence gathered from taphonomic assessments and a series of radiocarbon datings indicate that the sacrifices fall into three consecutive phases spanning several years. The findings of the zooarchaeological analyses clearly point to a selection of equid and cattle males. Adult equids predominate (MNI = 41) followed by adult and sub-adult cattle (MNI = 6). Pigs, in turn, are only represented by a few adults and sub-adult females (MNI = 4). Among the animals is a single dog of undetermined sex between 3 and 4 years of age. The fact that the animals are mostly adults discards the likelihood that they died from natural causes or an epidemic. In addition, the scenographic deposition of certain equids in pairs, as well as evidence of the burning of plant offerings, suggest an intentional ritualistic sacrifice. Nine of the initial depositions of Phase 1 in the SE quadrant were scattered and certain of their bones bear marks characteristic of both prolonged open air exposure and scavengers. Another 31 animals from Phases 1 and 2 are represented by almost complete, articulated skeletons, indicating they were promptly covered. Phase 3, by contrast, reveals both almost complete and partial animals bearing clear signs of processing for human consumption. This study thus sheds light on both the sequence of the animal sacrifices and the protocols linked to rites accompanied by the celebration of banquets. Certain features associated with the sealing of this building under a tumulus offer evidence of the decline of the Tartessian Culture. This study thus advances notions serving to contextualize ritual animal sacrifices in the framework of practice observed at other Iron Age sites in the Iberian Peninsula and elsewhere throughout Europe.

Long-term analysis of Zostera noltei: A retrospective approach for understanding seagrasses' dynamics.

Long-term studies are necessary to establish trends and to understand seagrasses' spatial and temporal dynamic. Nevertheless, this type of research is scarce, as the required databases are often unavailable. The objectives of this study are to create a method for mapping the seagrass Zostera noltei using remote sensing techniques, and to apply it to the characterization of the meadows' extension trend and the potential drivers of change. A time series was created using a novel method based on remote sensing techniques that proved to be adequate for mapping the seagrass in the emerged intertidal. The meadows seem to have a decreasing trend between 1984 and the early 2000s, followed by an increasing tendency that represents a recovery in the extension area of the species. This 30-year analysis demonstrated the Z. noltei's recovery in the study site, similar to that in other estuaries nearby and contrary to the worldwide decreasing behavior of seagrasses.

Long-term dynamics of a floodplain shallow lake in the Pantanal wetland: Is it all about climate?

Hydrological variability over seasonal and multi-annual timescales strongly shapes the ecological structure and functioning of floodplain ecosystems. The current IPCC climate scenario foresees an increase in the frequency of extreme events. This, in conjunction with other anthropogenic disturbances (e.g., river regulation or land-use changes) poses a serious threat to the natural functioning of these ecosystems. In this study we aimed to i) evaluate the long-term variability of the flooded area of the third largest floodplain lake in the Brazilian Pantanal using remote sensing techniques, and ii) analyze the possible factors influencing this variability. Changes in open-water and riparian floodplain-wetland vegetation areas were mapped by applying an ad hoc-developed remote-sensing method (including a newly developed normalized water index, NWI) to 221 Landsat-Thematic Mapper (TM)/Enhanced Thematic Mapper Plus (ETM+) images, acquired between 1984 and 2011. Added to the lake's natural swing between riparian floodplain-wetland vegetation expansion and retraction, our analyses revealed large interannual changes, grouped into three main periods within the studied time interval. Moreover, our results indicate that this floodplain-lake system is losing open-water area, paired with an increase in riparian floodplain-wetland vegetation. The system's long-term dynamics are not all climate related, but are the result of a combination of drivers. The start of the Manso dam's operation upstream of the studied system, and the subsequent river regulation because of the dam operation, coupled with climatic oscillation appear to be responsible for the observed changes. However, other factors which were not considered in this study might also be important in this process and contributing to the reduction of the system's resilience to droughts (e.g., land-use changes). This study illustrates the serious conservation risks that the Pantanal faces in the near future, given the current climate-change scenario and the accumulation of dam building projects in this region.