[Retrospective study of patients diagnosed with Sweet syndrome in the health area of a tertiary hospital in the autonomous community of Valencia]. / Estudio retrospectivo de pacientes diagnosticados de síndrome de Sweet en el área de un hospital terciario de la Comunidad Valenciana.

Sweet syndrome is the most characteristic of the neutrophilic dermatoses. We performed a retrospective study of patients with Sweet syndrome seen in our department between 2001 and 2009, inclusive; the aims were to define the patient profile and to evaluate the clinical and epidemiological differences between subgroups. There were 24 patients (13 women and 11 men). The age distribution was similar in both sexes and showed 2 peaks, one in the fourth decade and the other in the eighth decade. The etiology was predominantly infectious or inflammatory, followed by the idiopathic form. There were 4 cases of paraneoplastic disease, 2 of which involved solid-organ tumors. One case was associated with the administration of infliximab. Symptoms persisted longer in cases that were idiopathic or that developed in the context of neoplastic disease.

Identification of a novel keratinocyte mitogen derived from bovine pituitary glands.

Bovine pituitary glands contain one or more factors that are important for keratinocyte proliferation in serum-free culture medium. We used a tissue culture system in which the growth of keratinocytes in basal medium (KBM) containing insulin was dependent upon exogenous growth factors. Using this experimental system, we began to purify and characterize the pituitary factor(s) necessary for clonal growth of human keratinocytes in serum-free medium. Proteins of approximately 150 kDa and 95 kDa bound specifically to living keratinocytes, and we suggest that the 95 kDa protein is a likely novel mitogen. Although prolactin has been previously identified as a pituitary hormone that may act as an in vitro mitogen for keratinocytes, imunoblots indicated that the 95 kDa protein was unrelated to prolactin. Furthermore, the 95 kDa protein showed high homology with a bovine 90 kDa heat shock protein in the limited sequencing of an internal peptide.

[Vulvar lymphedema: unusual manifestation of metastatic Crohn's disease]. / Linfedema vulvar: una manifestación infrecuente de la enfermedad de Crohn metastásica.

Cutaneous-mucosal lesions constitute one of the most frequent extraintestinal manifestations of Crohn's disease and in some cases may be the first symptom of intestinal disease. We describe the case of a 45-year-old female patient who sought medical help for genital tumefaction of 20 years' evolution. For the previous 15 years, she had been experiencing digestive symptomatology attributed to irritable bowel syndrome. Two months before the consultation, and coinciding with aggravation of the condition, the patient had been diagnosed with colonic Crohn's disease. Skin biopsy of the labia minora revealed sarcoid granulomas. The results of microbiological studies (staining for microorganisms and cultures) were negative. A diagnosis of metastatic vulvar Crohn's disease was made and, treatment with metronidazole was started, which improved the genital edema after 2 months. Genital lymphedema is an exceptionally rare manifestation of metastatic Crohn's disease that may appear several years before intestinal symptomatology develops. Treatment with metronidazole seems to be a good therapeutic option.

[Pseudocysts of the auricle. Clinical and histological studies of 3 cases]. / Pseudo-kystes du pavillon auriculaire. Etude clinique et histologique de trois cas.

Three cases of pseudocyst of the auricle were studied. All three occurred in male, 38, 54, and 23 years old; all had a solitary nodule of the anthelix. Such pseudocyst have a characteristic localization and are not caused by trauma and have a serous, cystic content. The cystic formations develop from degeneration and liquefaction within the auricular cartilage. We believe that the name proposed by Engel "pseudocyst of the auricle" can be appropriated.

[Immunohistochemical study of the distribution of carcinoembryonic antigen and keratin in tumors of epidermal origin. II: Keratoacanthoma and premalignant lesions]. / Estudio inmunohistoquímico de distribución de antígeno carcinoembrionario y queratina en tumores de origen epidérmico. Parte II: Queratoacantoma y lesiones premalignas.

Carcinoembryonic antigen (CEA) has been studied extensively, in association with visceral tumors and normal tissues. Although CEA has been demonstrated in sweat gland cutaneous tumors, little is known about its presence in skin and epithelial related tumors. We studied the presence of CEA and keratin in keratoacanthomas and precancerous skin lesions using immunohistochemical techniques. Its distribution related with differentiation, proliferation and malignant potential is observed. The expression of both antigens is correlated.

[Immunohistochemical study of the distribution of carcinoembryonic antigen and keratin in 2 cases of extramammary Paget disease]. / Estudio inmunohistoquímico de distribución de antígeno carcinoembrinario y queratina en dos casos de enfermedad de Paget extramamario.

Sweat gland origin of Paget disease is demonstrated by the presence of carcinoembryonic antigen (CEA). We studied the expression of CEA and keratin in two cases of extramammary Paget disease. Our results are commented.

[Immunohistochemical study of the distribution of carcinoembryonic antigen and keratin in tumors of epidermal origin. I: Squamous and basal cell epitheliomas]. / Estudio inmunohistoquímico de distribución de antígeno carcinoembrionario y queratina en tumores de origen epidérmico. Parte I: Epiteliomas espinocelulares y basocelulares.

Carcinoembryonic antigen (CEA) has been studied extensively, in association with visceral tumors and normal tissues. Although CEA has been demonstrated in sweat gland cutaneous tumors, little is known about its presence in skin and epithelial related tumors. We studied the presence of CEA and keratin in squamous cell and basal cell carcinoma using immunohistochemical techniques. Its distribution related with differentiation, proliferation and malignant potential is observed. The expression of both antigens is correlated.

[Determination of B lymphocytes: immunofluorescence and peroxidase dyes (preliminary note)]. / Determinación de linfocitos B: inmunofluorescencia y tinción de peroxidasas. (Nota previa)

A determination of the lymphocyte levels in blood and cutaneous infiltrates of 60 patients complaining of different dermatoses were carried out by the authors. The method used in the experience was that of Preud'homme and Flandrin (1974). In most of the investigated cases the results could be considered as normal (8-22%). In one case of actinic reticuloid and 2 cases of plane xanthoma with photosensitivity the levels were considerably raised. The authors belleve that the B-lymphocytes determination could be worth investigation in the photosensitive dermatoses.

[Effect of bornaprine in localized hyperhidrosis]. / Efecto de la bornaprina en las hiperhidrosis localizadas.

Therapeutic action of bornaprine on localized forms of hyperhidrosis is studied in 12 patients. 50% improved after a week treatment. Other patients showed an acceptable change in the third or fourth week. Side effects only appeared in one patient.

[Argyria]. / Argiria.

A case of generalized argyria is reported a clinical study and literature review is made. The ultraestructural findings permit to establish a hypothesis on the silver grains morphology.

[Differentiation of human epidermis cultured in vitro]. / La diferenciación de la epidermis humana cultivada in vitro.

The authors studied human epidermal differentiation in normal skin and skin cultured according to Green's method. No differences was observed except for the filaggrin and involucrin patterns; the lectins revealed identical patterns in normal and cultured human epidermis. The ultrastructural study verified a similar morphology in both types of epidermis.

[Penile horn]. / Penile horn.

Two cases of penile horn are reported. This infrequent condition appears usually after a surgical circumcision. One of the cases presented with generalized metastasis. The nosology, prognosis and treatment of this entity is discussed.

[Study of the circulating lymphocytes populations in systemic scleroderma, chronic discoid lupus erythematosus and dermatomyositis]. / Estudio de las poblaciones linfocitarias circulantes en esclerodermia sistémica, lupus eritematoso discoide crónico y dermatomiositis.

The authors studied the peripheral blood lymphocytes in any autoimmune diseases. In scleroderma the T-lymphocytes and ratio T4/T8 are decreased and B-lymphocytes are normal. In the LEDC the T-lymphocytes are decreased and B-lymphocytes are increased. In dermatomyositis the B-lymphocytes are increased and T-lymphocytes are decreased, the ratio T4/T8 is normal.

[Immunologic parameters in patients with acuminata condylomas]. / Estudio de parámetros inmunológicos en pacientes con condilomas acuminados.

A study is made of the state of cell and humoral immunity in patients with genital warts. The results show an increase in the T-suppressor lymphocyte population (T8+) (p less than 0.011) of these patients, which reduces the T-helper/T suppressor ratio (T4 +/T8 +). No alterations are observed in the humoral immunity on analyzing seric immunoglobulin concentration (IgA, IgG, IgM), complement factors (C3, C4), or their functionality (CH50).

[Spinocellular epithelioma. Risk factors in the occurrence of metastasis. Study of 650 cases]. / Epitelioma espinocelular. Factores de riesgo en la aparición de metástasis. Estudio de 650 casos.

An evaluation is made of the clinical and histological parameters of 650 cases of spinocellular epitheliomas. The statistical study shows metastasis to be related to tumor location, tumor size, Broder's grading and acantholysis.

[Marking disorders of keratinization by means of lectins. II. Keratosis pilaris, lichen spinulosus, porokeratosis, lichen striatus and pityriasis rubra pilaris]. / Marcaje mediante lectinas de trastornos en la queratinización. II. Queratosis pilaris, liquen espinuloso, poroqueratosis, liquen estriado y pitiriasis rubra pilaris.

Lectins are used to study four cases of keratosis pilaris, four of lichen spinulosus, 11 of porokeratosis, 8 of striated lichen and one case of pityriasis rubra pilaris, with the aim of providing data to improve knowledge of the histogenesis of these processes.

[Lectin staining of a Merkel cell tumor]. / Marcaje mediante lectinas de un tumor de células de Merkel.

The authors studied a Merkel's cell tumor with lectins. There is pattern of normal epidermis, but we see the N-acetyl-glucosamine an oligosaccharide not present in epidermis.

[Lectin staining of disorders of keratinization. I. Ichthyosis, Darier's disease and acrokeratosis verruciformis]. / Marcaje mediante lectinas de transtornos de la queratinización. I. Ictiosis, enfermedad de Darier y acroqueratoris verruciforme.

Lectin application is used to study 12 cases of ichthyosis, 8 cases of Darier's disease and two of acrokeratosis verruciformis, establishing comparative patterns with normal skin.

[Plantar myofibromatosis]. / Miofibromatosis plantar.

A case of recurrent plantar myofibromatosis is described, in which myofibroblasts are shown ultrastructurally. The characteristics and properties of this presence in different types of fibromatosis are discussed.

[Expression of HLA-Dr and CD1+ cells in malignant and benign pigmentary tumors]. / Expresión del HLA-Dr y células CD1+ en tumores pigmentarios malignos y benignos.

The authors studied the expression of CD1 and HLA-Dr antigen in benign and malignant melanic tumors to known if were the melanoma's cells or Langerhans cells, presents on the tumor, which exhibited the HLA-Dr antigens, and if the HLA-Dr expression was related to volume, depth and metastatic characteristics. These antigens were studied with the monoclonal antibodies labelled to avidin-biotin-peroxidase system. The results shows than the proportion between the number of Langerhans cells and HLA-Dr positive cells is inverse. No differences were found between the expression of the HLA-Dr antigens and number of Langerhans cells and volume, depth or metastatic characteristics.