Depth plane adaptive integral imaging using a varifocal liquid lens array.

This paper proposes an enhanced integral imaging system with an electrically controllable image plane to address the issue of the limited depth problem in integral imaging. For implementation of the variable image plane, a varifocal liquid lens array and driving device are adopted instead of an ordinary solid lens array. The position of the central depth plane is varied by adjusting the focal length of the lens array. The proposed system enables matching between the object position and depth plane electrically, and thus an object moving from 5.15 to 11.72 cm is clearly displayed with this method.

The sagittal plane angle and tunnel-related complications in double-bundle anterior cruciate ligament reconstruction using the transportal technique: an in vivo imaging study.

PURPOSE: To evaluate the relation between the tunnel angle in the 3 orthogonal planes, especially the sagittal plane, which can be influenced by knee flexion during drilling, and the incidence of complications from the transportal technique using in vivo imaging data. METHODS: Fifty-one patients who underwent anatomic double-bundle anterior cruciate ligament reconstruction by the transportal technique were evaluated retrospectively. A 3-dimensional surface model was made using an axial computed tomography scan obtained after anterior cruciate ligament reconstruction. The tunnel length, posterior cortical damage, proximity between the outer orifice of the tunnel and lateral collateral ligament (LCL) origin, and medial femoral condyle cartilage damage were evaluated on a 3-dimensional computed tomography scan and 3-T magnetic resonance imaging. Correlations between those parameters and the tunnel angle in the coronal, axial, and sagittal planes were analyzed. RESULTS: A tunnel length of less than 30 mm developed in 4 cases (8%) in the anteromedial tunnel and in 1 case (2%) in the posterolateral (PL) tunnel. Posterior cortical damage developed in 12 cases (23%). A distance from the outer orifice of the tunnel to the LCL origin of less than 3 mm occurred in 18 cases (35.2%) in the PL tunnel. Medial femoral condyle cartilage damage was detected in 3 cases (6%). A positive correlation was observed between the sagittal angle and anteromedial tunnel length (P = .002, r = 0.547). The sagittal angle in the group with posterior cortical damage was lower than that in the group with no posterior cortical damage (P = .002). A negative correlation was observed between the distance from the outer orifice of the PL tunnel to the LCL origin and the sagittal angle (P = .002, r = -0.55). CONCLUSIONS: Drilling at a higher angle in the sagittal plane decreased the incidence of posterior cortical damage and a short anteromedial tunnel. However, drilling at a higher angle shortened the distance to the LCL origin for the PL tunnel. LEVEL OF EVIDENCE: Level IV, therapeutic case series.

The Effect of Hinge Position on Posterior Tibial Slope in Medial Open-Wedge High Tibial Osteotomy.

PURPOSE: To evaluate whether hinge position affects the change in posterior tibial slope in medial open-wedge high tibial osteotomy (HTO). METHODS: We retrospectively evaluated 19 knees from 17 patients who underwent medial open-wedge HTO by 3-dimensional computed tomography scan before and after surgery. A 3-dimensional image model was constructed by applying reverse-engineering software to the computed tomography DICOM (Digital Imaging and Communications in Medicine) files. The hinge axis (i.e., the position of the hinge compared with the anteroposterior axis on an axial view), posterior tibial slope, medial-proximal tibial angle, and gap ratio (i.e., the ratio of anterior gap to posterior gap in the opened wedge) were measured. RESULTS: The mean hinge axis was 4.92° ± 3.86°. Posterior tibial slope increased from 7.29° ± 2.56° preoperatively to 10.48° ± 3.01° postoperatively (P = .001). The mean medial-proximal tibial angle was 85.96° ± 1.97° preoperatively and 93.13° ± 3.17° postoperatively (P = .001). The mean gap ratio was 62.48% ± 7.26%. Linear regression analysis determined that the hinge axis (P = .0001) was a significant factor changing posterior tibial slope. CONCLUSIONS: Hinge position affected the change in posterior tibial slope in medial open-wedge HTO; in particular, a posterolateral hinge position led to an increase in posterior tibial slope. LEVEL OF EVIDENCE: Level IV, therapeutic study.

Effect of axial loading during knee flexion on ACL end-to-end distance in healthy and ACL-deficient knees.

PURPOSE: The purpose of this study was to determine the effect of physiological axial loading during knee flexion on changes in anterior cruciate ligament (ACL) end-to-end distance for normal and ACL-deficient knees. METHODS: Biomechanical tests were conducted on ten cadaveric knees using an Instron machine. We gathered positional data of the tibia and femur at low to middle flexion angles (0°, 15°, 30°, 45° and 60°) with/without axial loading. First, no external load was applied to the specimens at each angle, and then, a 1000-N axial load was applied to the knees. The same test protocols were repeated after transection of the ACL. Using computer software (Geomagic Studio 10), we regenerated positional data and calculated the end-to-end distances of the anteromedial, posterolateral and the entire ACL bundle at each angle. RESULTS: Compared with ACL-intact knees without axial loading, knees under axial loading did not show significant increases in end-to-end distance. Under axial loading, we found no significant differences in end-to-end distances between bundles in ACL-intact knees according to the increase in knee flexion angle. After ACL transection, axial loading significantly increased end-to-end distances of all three bundles (P < 0.001), and the distances increased significantly with flexion angle (P < 0.05 at all angles in all bundles). CONCLUSION: The changing patterns of the ACL end-to-end distance in ACL-deficient knees were different from those in healthy knees after applying physiological axial loading, and the ACL end-to-end distances in ACL-deficient knees increased remarkably as knee flexion angles increased.

Full-arch accuracy of five intraoral scanners: In vivo analysis of trueness and precision.

OBJECTIVE: To evaluate the trueness and precision of full-arch scans acquired using five intraoral scanners and investigate the factors associated with the dimensional accuracy of the intraoral scan data. METHODS: Nine adult participants (mean age, 34.3 ± 8.3 years) were recruited. Four zirconium spheres (Ø 6 mm) were bonded to the canines and the molars. Following acquisition of reference scans using an industrial-grade scanner, five intraoral scanners, namely i500, CS3600, Trios 3, iTero, and CEREC Omnicam, were used to scan the arches. Linear distances between the four reference spheres were automatically calculated, and linear mixed model analysis was performed to compare the trueness and precision of the intraoral scan data among the different scanners. RESULTS: The absolute mean trueness and precision values for all intraoral scanners were 76.6 ± 79.3 and 56.6 ± 52.4 µm, respectively. The type of scanner and the measured linear distances had significant effects on the accuracy of the intraoral scan data. With regard to trueness, errors in the intermolar dimension and the distance from the canine to the contralateral molar were greater with Omnicam than with the other scanners. With regard to precision, the error in the linear distance from the canine to the molar in the same quadrant was greater with Omnicam and CS3600 than with the other scanners. CONCLUSIONS: The dimensional accuracy of intraoral scan data may differ significantly according to the type of scanner, with the amount of error in terms of trueness being clinically significant.

Trophic Ecology of Endangered Gold-Spotted Pond Frog in Ecological Wetland Park and Rice Paddy Habitats.

The gold-spotted pond frog (Pelophylax chosenicus) is an endangered amphibian species in South Korea. In order to obtain ecological information regarding the gold-spotted pond frog's habitat environment and biological interactions, we applied stable isotope analysis to quantify the ecological niche space (ENS) of frogs including black-spotted pond frogs (P. nigromaculatus) and bullfrogs (Lithobates catesbeianus) within the food web of two different habitats-an ecological wetland park and a rice paddy. The gold-spotted pond frog population exhibited a broader ENS in the ecological wetland park than in the rice paddy. According to the carbon stable isotope ratios, gold-spotted pond frogs mainly fed on insects, regardless of habitat type. However, the results comparing the range of both carbon and nitrogen stable isotopes showed that gold-spotted pond frogs living in the rice paddy showed limited feeding behavior, while those living in the ecological wetland park fed on various food sources located in more varied trophic positions. Although the ENS of the gold-spotted pond frog was generally less likely to be overlapped by that of other frog species, it was predicted to overlap with a high probability of 87.3% in the ecological wetland park. Nevertheless, gold-spotted pond frogs in the ecological wetland park were not significantly affected by the prey competition with competitive species by feeding on other prey for which other species' preference was low. Since these results show that a habitats' food diversity has an effect on securing the ENS of gold-spotted pond frogs and prey competition, we recommend that the establishment of a food environment that considers the feeding behavior of gold-spotted pond frogs is important for the sustainable preservation of gold-spotted pond frogs and their settlement in alternative habitats.

A digital approach to dynamic jaw tracking using a target tracking system and a structured-light three-dimensional scanner.

PURPOSE: This technical procedure describes a method for tracking mandibular movement using a three-dimensional (3D) optical scanner and target tracking system to digitally portray the motion of the mandible and temporomandibular joints by merging cone beam computed tomography (CBCT) data. METHODS: Four nonreflective targets were attached to the labial surface of the incisors in a noncolinear arrangement. Mandibular movement was tracked directly using a 3D facial scanner and target tracking software after merging facial scanning data, digital data obtained from a diagnostic cast, and CBCT scan data based on several landmarks of the anterior teeth. The moving path of the subjects' mandible was converted to CBCT-based data to confirm the actual movement of the mandible and temporomandibular joints. CONCLUSIONS: The digital implementation of mandibular movement using a 3D optical scanner and target tracking system is not prone to the same restrictions and limitations inherent in mechanical equipment; therefore, it is possible to reconstruct more realistic movement(s). This technique can be used in a wide variety of dental applications involving movement of the mandibular jaw, such as fabrication of dental prostheses, or for the diagnosis and treatment of temporomandibular joint disease.

Comparison of anterior cruciate ligament volume after anatomic double-bundle anterior cruciate ligament reconstruction.

BACKGROUD: To determine whether anatomic double-bundle anterior cruciate ligament reconstruction (DB-ACLR) can restore the native ACL volume, and whether the volume change after reconstruction affects clinical outcomes and re-rupture rates following the contemporary techniques. METHODS: Eighty patients undergoing anatomic DB-ACLR using transportal or outside-in technique were prospectively evaluated with magnetic resonance imaging (MRI) before and after surgery. The ACL volumes were determined from 3-D models constructed by applying reverse engineering software. In all participants, measured reconstructed ACL volume were compared with the ACL on the opposite uninjured side. Participants were divided into two groups according to the volume of reconstructed graft; larger volume than native ACL of contra-lateral side (Group 1) or smaller (Group 2). RESULTS: The mean ACL volume on the reconstructed side (1726.5mm3, 982.1 - 2733.8) was significantly smaller than that on the uninjured opposite side (1857.6mm3, 958.2 - 2871.5) (P<0.001). A total of 31 patients in Group 1 and 49 in Group 2 showed no significant difference of improvement in the clinical outcome scales at the postoperative two-year follow-up (Lysholm knee score, P=0.830, Tegner activity score, P=0.848). Four patients with ACL re-rupture during the two-year follow-up after reconstruction had smaller reconstructed ACL volumes than native ligament on the opposite site. CONCLUSION: Anatomic DB-ACLR technique restored the graft volume rather smaller than the volume of the native ACL. Based on the volumetric consideration, graft reconstructed by anatomic DB-ACLR might have increased probability of re-rupture due to its smaller volume related to native ACL on the contralateral side.

Three-Dimensional Evaluation of Similarity of Right and Left Knee Joints.

PURPOSE: The purpose of this study was to evaluate the anatomical similarity of three-dimensional (3D) morphometric parameters between right and left knees. MATERIALS AND METHODS: Ten fresh-frozen paired cadaveric knees were tested. Following dissection, footprint areas of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) were measured. Surface scanning was performed using a 3D scanner. Scanned data were reproduced and morphometric parameters were measured on specialized software. After making mirror models, we compared footprint center positions of the ACL and PCL of both sides and calculated the average deviation of 3D alignment between the right- and left-side models. RESULTS: No significant side-to-side differences were found in any morphometric parameters. Bony shapes displayed a side-to-side difference of 〈1 mm. Distal femoral and proximal tibial volumes did not present side-to-side differences, either; the average 3D deviations of alignment between the right and left sides were 0.8±0.4/1.1±0.6 mm (distal femur/proximal tibia). Center-to-center distances between the right and left ACL footprints were 2.6/2.7 mm (femur/tibia) for the anteromedial bundle and 2.4/2.8 mm for the posterolateral bundle. They were 1.9/1.5 mm for the anterolateral bundle and 2.2/1.8 mm for the posteromedial bundle of the PCL. CONCLUSIONS: There was a remarkable 3D morphometric similarity between right and left knees. Our results might support the concept of obtaining morphologic reference data from the uninvolved contralateral knee.

Evaluation of the accuracy and precision of four intraoral scanners with 70% reduced inlay and four-unit bridge models of international standard.

The aims of this study were to evaluate the feasibility of 70% reduced inlay and 4-unit bridge models of International Standard (ISO 12836) assessing the accuracy of laboratory scanners to measure the accuracy of intraoral scanner. Four intraoral scanners (CS3500, Trios, Omnicam, and Bluecam) and one laboratory scanner (Ceramill MAP400) were used in this study. The height, depth, length, and angle of the models were measured from thirty scanned stereolithography (STL) images. There were no statistically significant mean deviations in distance accuracy and precision values of scanned images, except the angulation values of the inlay and 4-unit bridge models. The relative errors of inlay model and 4-unit bridge models quantifying the accuracy and precision of obtained mean deviations were less than 0.023 and 0.021, respectively. Thus, inlay and 4-unit bridge models suggested by this study is expected to be feasible tools for testing intraoral scanners.

Three-dimensional relationships between secondary changes and selective osteotomy parameters for biplane medial open-wedge high tibial osteotomy.

BACKGROUND: To assess the axial rotational change of distal tibia and posterior tibial slope (PTS) change after OWHTO in 3-D planes and to identify the causal relationship on the effect of variation in the posterior slope angle and rotational errors. METHODS: A total of 21 patients (23 knees) underwent OWHTO and were evaluated with 3D-CT before and after surgery. Medial proximal tibial angle in the coronal plane, PTS in the sagittal plane, and rotational axis in axial plane were evaluated and compared between pre- and post-operative 3D models constructed by applying reverse-engineering software. As a selective osteotomy parameter, hinge axis and gap ratio were measured in the postoperative 3D models RESULTS: The increasing tendency of internal rotation of the distal tibia after OWHTO was positively related to hinge axis (ß=0.730, p=0.001, R2=0.546) and gap ratio (ß=-0.283, p=0.001, R2=0.520), which also showed statistically significant linear correlations to PTS changes after multivariate regression analysis that controlled for the rotational change of the distal tibia (hinge axis: ß=0.443, p=0.006; gap ratio: ß=0.144, p=0.017). CONCLUSION: Hinge axis more posterolaterally was related to a greater increase in internal rotation after biplane medial open-wedge HTO, and hinge axis and gap ratio were significant predictors of PTS change after rotational change was controlled for. Hinge axis has to be considered an important independent variable for limiting unintended axial rotation change as well as PTS change as secondary. CLINICAL RELEVANCE: The relationship of the hinge axis with the rotational change and its influence to PTS change, acknowledged from by-product of the statistical analysis, might provide a deeper understanding of HTO, and should have constitutional effects on the development of HTO procedures and implants.

Mitochondrial genome sequence of Sibynophis chinensis (Squamata, Colubridae).

The complete mitochondrial (mt) genome of the Chinese many-toothed snake, Sibynophis chinensis, was sequenced and found to be 17,163 bp in length. The arrangement of 13 protein-coding genes, tRNAs and rRNAs was identical to that of other common snake mt genomes. The mt protein-coding genes of S. chinensis utilized ATA, ATG, ATA and GTG as initiation codons and AGA, AGG, TAA, TAG and T as termination codons. Among three tRNA clusters (LQM, WANCY and HSL), LQM was found instead of IQM, which is common in other vertebrates. We also identified two control regions that contained several conserved elements known as conserved sequence blocks and termination-associated sequences related to mt replication and transcription.

Volume and contact surface area analysis of bony tunnels in single and double bundle anterior cruciate ligament reconstruction using autograft tendons: in vivo three-dimensional imaging analysis.

BACKGROUND: Regarding reconstruction surgery of the anterior cruciate ligament (ACL), there is still a debate whether to perform a single bundle (SB) or double bundle (DB) reconstruction. The purpose of this study was to analyze and compare the volume and surface area of femoral and tibial tunnels during transtibial SB versus transportal DB ACL reconstruction. METHODS: A consecutive series of 26 patients who underwent trantibial SB ACL reconstruction and 27 patients with transportal DB ACL reconstruction using hamstring autograft from January 2010 to October 2010 were included in this study. Three-dimensional computed tomography (3D-CT) was taken within one week after operation. The CT bone images were segmented with use of Mimics software v14.0. The obtained digital images were then imported in the commercial package Geomagic Studio v10.0 and SketchUp Pro v8.0 for processing. The femoral and tibial tunnel lengths, diameters, volumes and surface areas were evaluated. A comparison between the two groups was performed using the independent-samples t-test. A p-value less than the significance value of 5% (p < 0.05) was considered statistically significant. RESULTS: Regarding femur tunnels, a significant difference was not found between the tunnel volume for SB technique (1,496.51 ± 396.72 mm(3)) and the total tunnel volume for DB technique (1,593.81 ± 469.42 mm(3); p = 0.366). However, the total surface area for femoral tunnels was larger in DB technique (919.65 ± 201.79 mm(2)) compared to SB technique (810.02 ± 117.98 mm(2); p = 0.004). For tibia tunnels, there was a significant difference between tunnel volume for the SB technique (2,070.43 ± 565.07 mm(3)) and the total tunnel volume for the DB technique (2,681.93 ± 668.09 mm(3); p ≤ 0.001). The tibial tunnel surface area for the SB technique (958.84 ± 147.50 mm(2)) was smaller than the total tunnel surface area for the DB technique (1,493.31 ± 220.79 mm(2); p ≤ 0.001). CONCLUSIONS: Although the total femoral tunnel volume was similar between two techniques, the total surface area was larger in the DB technique. For the tibia, both total tunnel volume and the surface area were larger in DB technique.

In vivo three-dimensional imaging analysis of femoral and tibial tunnel locations in single and double bundle anterior cruciate ligament reconstructions.

BACKGROUND: Anatomic footprint restoration of anterior cruciate ligament (ACL) is recommended during reconstruction surgery. The purpose of this study was to compare and analyze the femoral and tibial tunnel positions of transtibial single bundle (SB) and transportal double bundle (DB) ACL reconstruction using three-dimensional computed tomography (3D-CT). METHODS: In this study, 26 patients who underwent transtibial SB ACL reconstruction and 27 patients with transportal DB ACL reconstruction using hamstring autograft. 3D-CTs were taken within 1 week after the operation. The obtained digital images were then imported into the commercial package Geomagic Studio v10.0. The femoral tunnel positions were evaluated using the quadrant method. The mean, standard deviation, standard error, minimum, maximum, and 95% confidence interval values were determined for each measurement. RESULTS: The femoral tunnel for the SB technique was located 35.07% ± 5.33% in depth and 16.62% ± 4.99% in height. The anteromedial (AM) and posterolateral (PL) tunnel of DB technique was located 30.48% ± 5.02% in depth, 17.12% ± 5.84% in height and 34.76% ± 5.87% in depth, 45.55% ± 6.88% in height, respectively. The tibial tunnel with the SB technique was located 45.43% ± 4.81% from the anterior margin and 47.62% ± 2.51% from the medial tibial articular margin. The AM and PL tunnel of the DB technique was located 33.76% ± 7.83% from the anterior margin, 45.56% ± 2.71% from the medial tibial articular margin and 53.19% ± 3.74% from the anterior margin, 46.00% ± 2.48% from the medial tibial articular margin, respectively. The tibial tunnel position with the transtibial SB technique was located between the AM and PL tunnel positions formed with the transportal DB technique. CONCLUSIONS: Using the 3D-CT measuring method, the location of the tibia tunnel was between the AM and PL footprints, but the center of the femoral tunnel was at more shallow position from the AM bundle footprint when ACL reconstruction was performed by the transtibial SB technique.

Are navigation systems accurate enough to predict the correction angle during high tibial osteotomy? Comparison of navigation systems with 3-dimensional computed tomography and standing radiographs.

BACKGROUND: Unpredicted overcorrection of the mechanical axis can occur during navigation-assisted high tibial osteotomy (HTO). It is not clear whether the erroneous overcorrection stems from the navigation system itself or from other causes. PURPOSE: To evaluate the accuracy of the navigation system in HTO by comparing the change in the femorotibial angle provided by the navigation system with the bony correction angle of the proximal tibia on 3-dimensional computed tomography (3D CT) and with the change in mechanical femorotibial alignment on standing whole-leg radiographs. STUDY DESIGN: Cohort study (diagnosis); Level of evidence, 2. METHODS: A total of 16 knees underwent navigation-assisted HTO, and their alignment data were obtained before and after correction. For comparison, preoperative and follow-up standing whole-leg anteroposterior radiographs, lateral knee radiographs, and preoperative and postoperative 3D CT scans were taken. The medial proximal tibial angle (MPTA), posterior tibial slope, and mechanical femorotibial angle (mFTA) were measured in these images, and the 3 coronal and 3 sagittal correction angles were compared with each other. RESULTS: In the coronal plane, the mean correction angle of the navigation system was 9.3° ± 2.0° valgus (range, 6°-13°), and the mean MPTA on 3D CT increased 9.7° ± 2.0° (range, 6.7°-13.8°) after correction. The mean correction angle of the mFTA on standing radiographs was 11.9° ± 3.2° valgus (range, 6.9°-16.5°). There was no statistical significance between the navigation system and 3D CT (P = .187), but there was a statistically significant difference between the navigation system and standing radiographs (P = .001). The results of the correction angle in the sagittal plane were similar to those in the coronal plane. CONCLUSION: The correction of the femorotibial angle by the navigation system was not different from the bony correction angle on 3D CT. There was a discrepancy between the correction angle of the navigation system and that of the standing radiograph. CLINICAL SIGNIFICANCE: Surgeons must be cautious about the tension of soft tissue, even when using the navigation system during HTO.

Anatomic double-bundle reconstruction techniques result in graft obliquities that closely mimic the native anterior cruciate ligament anatomy.

BACKGROUND: It has been reported previously that single-bundle anterior cruciate ligament (ACL) reconstruction with more accurate restoration of the footprint of the native ACL fails to restore the graft obliquity of the native ACL in the coronal plane. Whether double-bundle ACL reconstruction restores the graft obliquity of each bundle of the native ACL has not yet been determined. HYPOTHESIS: Anatomic double-bundle ACL reconstruction using transportal (TP) and outside-in (OI) techniques can restore the graft obliquities of both anteromedial (AM) and posterolateral (PL) bundles in the native ACL in both sagittal and coronal planes. STUDY DESIGN: Cohort study; Level of evidence, 2. METHODS: Seventy-six patients underwent anatomic double-bundle ACL reconstruction. Patients were randomized to 2 groups to undergo either TP or OI tibial tunnel-independent anatomic ACL reconstruction. All participants underwent postoperative magnetic resonance imaging of both knees, and the native ACL obliquity was determined using the participant's contralateral knee. Graft obliquities were determined by measuring the angles between the center line of the graft and the surface line of the tibial plateau, and the obliquities of paired knees were compared. Graft obliquities were analyzed in each group. RESULTS: In the sagittal plane, the mean AM graft obliquity was 54.2° on the operated side and 54.6° on the nonoperated side, and the difference between sides was not statistically significant (P = .352). The mean PL graft obliquity in the sagittal plane was 54.1° on the operated side and 53.0° on the nonoperated side, and the difference between sides was also not statistically significant (P = .228). In the coronal plane, the mean AM graft obliquity was 73.8° on the operated side and 73.4° on the nonoperated side, and the mean PL graft obliquity was 65.5° on the operated side and 66.4° on the nonoperated side. There were no statistically significant differences between sides (P = .418 for AM graft; P = .328 for PL graft). Differences in the paired graft obliquities in each group were also statistically insignificant between the TP and OI groups. CONCLUSION: Both TP and OI anatomic double-bundle reconstruction techniques can result in graft obliquities in both bundles that resemble the native ACL in both sagittal and coronal planes. CLINICAL RELEVANCE: Double-bundle ACL reconstruction techniques might more closely restore the normal kinematics of the native ACL by restoring the normal obliquity of both ACL bundles.

Migration route estimation of the Jeju striped field mouse Apodemus agrarius chejuensis (Rodentia, Muridae).

The taxonomic relationship between two Korean field mice species, Apodemus agrarius coreae and A. a. chejuensis, as well as their possible historic migration routes, was examined by molecular genetic analysis of the complete mitochondrial cytochrome b gene of 73 mice collected from the Korean Peninsula and Jeju Island. Our findings suggest that A. a. coreae and A. a. chejuensis populations expanded and dispersed rapidly. Bayesian and network analysis showed that A. a. chejuensis is a clearly distinct population, and that A. a. chejuensis originated from the ancestral lineage of A. a. coreae. Based on our data, we hypothesize that the A. a. coreae population originated from eastern China or elsewhere. After the last glacial epoch, the lineage isolated from A. a. coreae had adapted to the new environment of Jeju Island, and with the reproductive isolation caused by the geographic barrier, this lineage eventually became a distinct population.

Mitochondrial genome sequences of the striped field mice Apodemus agrarius coreae and Apodemus agrarius chejuensis.

We determined the complete mitochondrial (mt) genome sequences of the striped field mice Apodemus agrarius coreae and Apodemus agrarius chejuensis. The mt genomes of A. a. coreae and A. a. chejuensis are 16,260 and 16,261 base pairs in length, respectively. The general features of the 13 protein-coding genes of the two species are similar to those of other rodents. The TAG termination codon for NADH dehydrogenase subunit (ND) 3 is unique to Apodemus in the Muroidea. The L-strand replication origin has the potential to form a stable stem-loop structure. Within the control region, a termination-associated sequence and several conserved sequence blocks were observed. The diversity of the 13 protein-coding genes, 2 rRNAs, and 1 control region between the two species ranged between 0.005 (ATP8) and 0.027 (ND4L).

The mitochondrial genome of Apodemus peninsulae (Rodentia, Muridae).

The complete mitochondrial (mt) genome of the Korean field mouse Apodemus peninsulae was sequenced and found to be 16,266 bp in length. The mt protein-coding genes of A. peninsulae had ATG, GTG, ATC, and ATA as initiation codons and TAA, TAG, TA, and T as termination codons. Two forms each of trnL and trnS and the three tRNA clusters, IQM, WANCY, and HSL were identified, as in the typical Rodentia mt genome. Among tRNAs, abnormal cloverleaf structure of trnS((AGY)) was identified in DHU arm. The l-strand replication origin has the potential to form a stable stem-loop structure and control region has several conserved sequence elements.