High-accuracy calibration method for an underwater one-mirror galvanometric laser scanner.

Three-dimensional (3D) perception of deep-sea targets is the key to autonomous operation of underwater equipment (e.g., underwater robots). Underwater one-mirror galvanometric line-laser scanner has advantages for short-range measurement, but it is difficult to achieve high calibration accuracy due to installation errors and refraction effects. For this reason, in this paper, a high-accuracy refraction-considered and installation-error-independent calibration method is proposed for the vision system. Firstly, to address the difficulty of aligning the incident light plane with the galvanometer shaft, a high-accuracy land-based installation-error-independent model is proposed, which avoids the influence of the installation errors and allows the real shaft axis and the light-plane cluster poses to be calculated using only three light planes. Subsequently, considering the underwater refraction, a 3D model is established for simulating refractive behaviors of the light-plane cluster, and then a partition-based method is proposed for calibrating the underwater light-plane cluster, which further improves the calibration accuracy of the scanner in underwater measurement scenarios. Finally, a one-mirror galvanometric laser scanner is developed in the laboratory to verify the calibration accuracy and to perform the 3D measurement experiments of underwater targets. The results show that the calibration accuracy of the proposed land-based installation-error-independent model is improved 2 times more compared with the traditional installation-error-dependent model. Additionally, the measurement accuracy of the scanner for the standard sphere is 11.98 µm and 12.75 µm in the air and underwater measurement scenarios, and the two measurements are in good agreement. The above results comprehensively verify the high accuracy of the calibration method proposed in this paper.

Single-lens multi-mirror laser stereo vision-based system for measuring internal thread geometrical parameters.

Oilfield pipes with out-of-tolerance internal thread can lead to failures, so the internal thread geometric parameters need to be measured. To tackle the problem of the low efficiency, poor accuracy, easy wear, and poor accessibility of existing methods, a single-lens multi-mirror laser stereo vision-based system for measuring geometric parameters of the internal thread is proposed, which allows the measurement of three parameters in one setup by completely reproducing the three-dimensional (3D) tooth profiles of the internal thread. In the system design, to overcome the incomplete representation of imaging parameters caused by insufficient consideration of dimensions and structural parameters of the existing models, an explicit 3D optical path model without a reflecting prism is first proposed. Then, considering the intervention of the reflecting prism, a calculation model for the suitable prism size and the final imaging parameters of the vision system is proposed, which ensures the measurement accessibility and accuracy by solving the problem that the existing system design only depends on experience without theoretical basis. Finally, based on the American Petroleum Institute standard, internal thread geometric parameters are obtained from the vision-reconstructed 3D tooth profiles. According to the optimized structural parameters, a vision system is built for measuring the internal thread geometric parameters of two types of oilfield pipes. Accuracy verification and typical internal thread measurement results show that the average measurement errors of the vision system proposed for the pitch, taper, and tooth height are 0.0051 mm, 0.6055 mm/m, and 0.0071 mm, respectively. Combined with the vision measurement time of 0.5 s for the three parameters, the above results comprehensively verify the high accuracy and high efficiency of the vision-based system.

Vision measurement system for geometric parameters of tubing internal thread based on double-mirrored structured light.

Geometric parameter measurement of tubing internal thread is critical for oil pipeline safety. In response to the shortcomings of existing methods for measuring internal thread geometric parameters, such as low efficiency, poor accuracy, and poor accessibility, this paper proposes a vision system for measuring internal thread geometric parameters based on double-mirrored structured light. Compared to previous methods, our system can completely reproduce the internal thread tooth profiles and allows multi-parameter measurement in one setup. To establish the correlation between the structural and imaging parameters of the vision system, three-dimensional (3D) optical path models (OPMs) for the vision system considering the mirror effect of the prism is proposed, which extends the scope of the optical path analysis and provides a theoretical foundation for designing the structural parameters of the vision system. Moreover, modeling and three-step calibration methods for the vision system are proposed to realize high-accuracy restoration from the two-dimensional (2D) virtual image to the actual 3D tooth profiles. Finally, a vision measurement system is developed, and experiments are carried out to verify the accuracy and measure the three geometric parameters (i.e., taper, pitch, and tooth height) of typical internal threads. Based on the validation results using the reference system, the vision measurement accuracy and efficiency are 6.7 and 120 times that of the traditional system, which verifies the measurement effectiveness and accuracy of the vision system proposed in this paper.

[Chemical constituents from fruits of Vitex trifolia var. simplicifolia].

The present study is to investigate the chemical constituents from the dried ripe fruits of Vitex trifolia var. simplicifolia The compounds were isolated by using a variety of chromatographic methods including silicagel, ODS, Sephadex LH-20, reversed-phase HPLC, and other methods. Their structures were identified by NMR, and MS date. As a result, 18 compounds were isolated and identified as ent-2-oxo15,16,19-trihydroxypimar-8(14)-ene (1), chrysosplenol D (2), casticin (3), luteolin (4), eupatrin (5), apigenin (6), 5,4'-dihydroxy-3,6,7-trimethoxyflavone (7), luteolin-4'-O-glucoside (8), hypolaetin-7-O-ß-D-glucopyranoside (9), swertisin (10), agestricin D (11), 5,3'-dihydroxy-6,7,4'-trimethoxyflavanone (12), tomentic acid (13), 2α,3ß,23-trihydroxyolean-12-en-28-oic acid (14), 3'-acetoxy-4'-angeloyloxy-3',4'-dihydroseselin (15), dihydrodehydrodiconiferyl alcohol (16), 3,5'-dimethoxy-4',7-epoxy-8,3'-neolignane-5,9,9'-triol (17) and salicifoliol (18). Among them, compounds 1, 2, 5-15, 17 and 18 were obtained from V. trifolia var. simplicifolia Cham for the first time and compounds 1, 5, 7-11, 15, 17 and 18 were isolated from thegenus Vitex for the first time.

[Sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi].

To study sesquiterpenes with anti-metastasis breast cancer activity from Chloranthus henryi, ten sesquiterpenes ,zedoarofuran (1), chlorajapolide D (2), 4ß, 8ß-dihydroxy-5α(H)-eudesm-7(11)-en-8, 12-olide (3), curcolonol (4), lasianthuslactone A (5), chlomultin C (6), (1E,4Z)-8-hydroxy-6-oxogermacra-1(10), 4, 7(11) -trieno-12, 8-lactone (7), shizukanolide E (8) , shizukanolide F (9) , 9α-hydroxycurcolonol (10), and five bis-sesquiterpenes, shizukaol B (11), shizukaol C (12) , cycloshizukaol A (13) , sarcandrolide B (14) , henriol A(15), were isolated by using different kinds of column chromatography methods from the ethyl acetate part of Ch.henryi and their structures were identified based on spectroscopic methods. Compounds 2, 8, 9, and 10 were obtained from the genus Chloranthus for the first time. Compounds 2, 5, 8-10, 12,and 14 were obtained from this plant for the first time. Some isolated compounds were subjected to evaluate the anti-metastasis breast cancer activity by using pharmacological methods, and only compounds 4, 11, and 12 were potent active.

[Study on chemical constituents from Chloranthus multistachys].

To investigate the chemical constituents from the shoots of Chloranthus multistachys.All compounds wereisolated by using a combination of various chromatographic techniques including silica gel, ODS, Sephadex LH-20, reversed-phase HPLC, and other methods.Their structures were elucidated by the nuclear magnetic resonance (NMR), mass spectrometry, and other modernspectroscopies.As a result, 19 compounds were isolated from the shoots of C.multistachys and identified as zederoneepoxide(1), chlomultin C(2), curcolonol(3), sarcaglaboside A(4), zedoarofuran(5), (1E,4Z)-8-hydroxy-6-oxogermacra-1(10), 4,7(11)-trieno-12,8-lactone(6), chloranoside A(7), istanbulin A(8), (8α)-6,8-dihydroxycadina-7(11),10(15)-dien-12-oicacid-γ-lactone(9), codonolactone(10), lasianthuslactone A(11), 12,15-epoxy-5αH,9ßH-labda-8(17),13-dien-19-oicacid(12), 12R,15-dihydroxylabda-8(17),13E-dien-19-oicacid(13), N-transcinnamoyltyramine(14), trans-N-p-coumaroyltyramine(15), dibutyl phthalate (16), flavokawain A(17), bergenin(18), and enedione(19).Compounds 1, 2, 4, 7-10, 12-19 were isolated from C.multistachys for the first time and compounds 14-19 were obtained from the genus Chloranthus for the first time.

Exploring the Therapeutic Potential of Triptonide in Salivary Adenoid Cystic Carcinoma: A Comprehensive Approach Involving Network Pharmacology and Experimental Validation.

BACKGROUND: Salivary Adenoid Cystic Carcinoma (ACC) is characterized by a highly invasive and slow-growing pattern, and its etiology remains unidentified. Triptonide (TN) has demonstrated efficacy as a pharmacotherapeutic agent against ACC. Nonetheless, the specific targets and mechanism of molecular action underlying the effectiveness of TN in treating ACC have not been elucidated. OBJECTIVES: By integrating network pharmacology with in-laboratory experiments, this research delves into the prospective targets and molecular mechanisms associated with the application of TN in treating ACC. METHODS: Initially, pertinent targets associated with TN against ACC were acquired from public databases. Subsequently, a combination of network pharmacology and bioinformatics analysis was utilized to screen the top 10 hub targets and key signal pathways of TN-treating ACC. Finally, in vitro experiments involving various molecular assays were conducted to evaluate the biological phenotypes of cells following TN treatment, encompassing assessments of apoptosis levels, plate migration, and other parameters, thereby validating pivotal genes and pathways. RESULTS: A total of 23 pertinent targets for TN in relation to ACC were identified, with the top 10 hub genes being MAPK8, PTGS2, RELA, MAPK14, NR3C1, HDAC1, PPARG, NFKBIA, AR, and PGR. There was a significant correlation between the TNF signaling pathway and the treatment of ACC with TN. In vitro experiments demonstrated that TN treatment elevated RELA phosphorylation while concurrently reducing MAPK14 phosphorylation and inducing G2/M arrest. TN exhibited the ability to enhance the apoptosis rate through increased caspase-3 activity, elevated levels of Reactive Oxygen Species (ROS), mitochondrial dysfunction, and inhibition of cell migration. CONCLUSION: There is a potential therapeutic role for TN in the treatment of ACC through the activation of the TNF signaling pathway. Among the identified candidates, MAPK8, HDAC1, PTGS2, RELA, NR3C1, PPARG, NFKBIA, AR, and PGR emerge as the most pertinent therapeutic targets for TN in the context of ACC treatment.

TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis.

Tribbles pseudokinase 3 (TRIB3) has been identified recently as a novel oncogene in several cancers. Still, further extensive research is imperative to elucidate its function and the molecular mechanisms underlying its involvement in the progression of head and neck squamous cell carcinoma (HNSCC). In our study, we found that TRIB3 silencing significantly promoted cell death by inducing ferroptosis. The interaction of TRIB3 with Transcription Factor 4 (TCF4) and ß-catenin created a heterotrimeric complex, which directly interacts with the ALOXE3 promoter, detrimentally impacting its activation. The consequential partial neutralization of ferroptosis induced by TRIB3 deficiency is observed through the implementation of ALOXE3 knockdown. Furthermore, the study demonstrated that the molecular inhibitor hesperidin, targeting TRIB3, not only reduced cell malignancy but also induced ferroptosis, thereby suppressing tumor growth. Overall, our findings unequivocally validate the proposition that TRIB3 deficiency precipitates the iron death mechanism, thereby indicating that the strategic targeting of TRIB3 could emerge as an innovative therapeutic strategy for HNSCC.

Evaluation of Biological Effects and Transcriptome Changes Induced by LED-Based Narrow-Band UVB Phototherapy.

Ultraviolet (UV), particularly UVB, is widely used in the treatment of skin diseases including psoriasis, atopic dermatitis, vitiligo, mycosis fungoides and pruritus. Recently, there has been a trend of replacing broad-band UVB (BB-UVB) units with narrow-band UVB (NB-UVB), as studies have demonstrated that NB-UVB is more efficacious in the treatment of psoriasis. The purpose of this study is to evaluate the biological effects and transcriptome changes induced by light-emitting diode-based NB-UVB (NB-UVB LED) phototherapy. Cell viability and the cell migration ability were significantly decreased posttreatment, as well as apoptosis and ROS levels were remarkably increased. NB-UVB-induced S phase arrest was observed 12 h postirradiation. Bioinformatics analysis of transcriptome sequencing data revealed that NB-UVB LED irradiation induced dose-depended changes in multiple key signaling pathways, such as PI3K and cytoskeletal-related pathways. The depolymerization of cytoskeleton induced by NB-UVB was observed 24 h posttreatment. In addition, the expression levels of cytoskeleton-related proteins FN1, ITGB4, ITGA1, RAC2 and DOCK1 decreased significantly 12 h after irradiation. Our results indicated that NB-UVB LED may serve as a novel option for the development of NB-UVB phototherapy devices.

Long Noncoding RNA CRYBG3 Blocks Cytokinesis by Directly Binding G-Actin.

The dynamic interchange between monomeric globular actin (G-actin) and polymeric filamentous actin filaments (F-actin) is fundamental and essential to many cellular processes, including cytokinesis and maintenance of genomic stability. Here, we report that the long noncoding RNA LNC CRYBG3 directly binds G-actin to inhibit its polymerization and formation of contractile rings, resulting in M-phase cell arrest. Knockdown of LNC CRYBG3 in tumor cells enhanced their malignant phenotypes. Nucleotide sequence 228-237 of the full-length LNC CRYBG3 and the ser14 domain of ß-actin is essential for their interaction, and mutation of either of these sites abrogated binding of LNC CRYBG3 to G-actin. Binding of LNC CRYBG3 to G-actin blocked nuclear localization of MAL, which consequently kept serum response factor (SRF) away from the promoter region of several immediate early genes, including JUNB and Arp3, which are necessary for cellular proliferation, tumor growth, adhesion, movement, and metastasis. These findings reveal a novel lncRNA-actin-MAL-SRF pathway and highlight LNC CRYBG3 as a means to block cytokinesis and to treat cancer by targeting the actin cytoskeleton.Significance: Identification of the long noncoding RNA LNC CRYBG3 as a mediator of microfilament disorganization marks it as a novel therapeutic antitumor strategy. Cancer Res; 78(16); 4563-72. ©2018 AACR.

Long non-coding RNA CRYBG3 regulates glycolysis of lung cancer cells by interacting with lactate dehydrogenase A.

Cancer cells usually utilize glucose as a carbon source for aerobic glycolysis, a phenomenon known as the Warburg effect. And a high rate of glycolysis has been observed in lung cancer cells. The growing evidence indicates that long non-coding RNAs (lncRNAs) are important players in lung cancer initiation and progression. However, the correlation between lncRNAs and glycolysis remains unclear. In this study, we recognized a lncRNA, LNC CRYBG3, which can interact with lactate dehydrogenase A (LDHA), a vital enzyme of glycolysis, is highly upregulated in both clinical lung cancer tissues and in vitro cultured lung cancer cell lines. A positive correlation between the expression level of LNC CRYBG3 and LDHA expression levels is observed. In another hand, LNC CRYBG3 is a regulator of glycolysis and its overexpression promoted the uptake of glucose and the production of lactate whereas the knockdown of LNC CRYBG3 led to opposite results and suppressed cell proliferation. These results indicated that LNC CRYBG3 might be a novel target for lung cancer treatment.

[Clinical application and evaluation of the custom shade guide of tetracycline stained teeth].

OBJECTIVE: To evaluate the clinical application of the custom shade guide of tetracycline stained teeth in color matching. METHODS: Forty-two patients with 59 tetracycline stained teeth were included in this study. Color matching was performed with Shofu shade guide and custom shade guide of tetracycline stained teeth. According to the two results, two porcelain fused to metal crowns were fabricated for each tooth. Evaluations were made both visually by dentists and patients and with ShadeEye NCC. RESULTS: Color difference between prostheses made according to custom shade guide and natural teeth was (7.80 +/- 4.70). Color difference between prostheses made according to Shofu shade guide and natural teeth was (10.68 +/- 4.70). Both visual evaluation and ShadeEye NCC evaluation showed that the custom shade guide provided a more accurate shade selection than the Shofu shade guide did, and the difference between the two shade guides was significant (t = 7.328, P < 0.001). CONCLUSIONS: The custom shade guide of tetracycline stained teeth provided a standard for clinical shade matching for the tetracycline stained teeth and could be a supplement to Shofu shade guide.