Salidroside Promotes Sensitization to Doxorubicin in Human Cancer Cells by Affecting the PI3K/Akt/HIF Signal Pathway and Inhibiting the Expression of Tumor-Resistance-Related Proteins.

Salidroside (Sal), the major active constituent of Rhodiola rosea L., is considered as a potential pro-drug with various activities; however, its role in tumor therapy is not clear. Here, we demonstrated in vitro and in vivo that Sal enhanced the inhibitory activity of doxorubicin (DOX) in drug-resistant cancer cell lines. Our results showed that combination drug treatment (Sal and DOX) significantly decreased cell proliferation, migration, and motility. Besides biological validation, a luciferase-labeled animal tumor xenograft model and bioluminescence imaging (BLI) were applied for assessing the tumor progression. Sal combined with DOX inhibited the growth of HeLa-ADR-luc cells in vivo and downregulated the DOX-induced high expression of MDR1. Also, Sal downregulated the Bcl-2, MMP-2, MMP-9, PI3K, and AKT and upregulated BAX proteins. Sal demonstrated high safety and cardiac protection activity. We discovered that Sal enhances DOX sensitivity through the regulation of PI3K/Akt/HIF-1α and DOX-induced resistance pathways. Our results suggest that Sal could be a novel chemosensitization agent for the treatment of multi-drug-resistance tumors.

Impact Behavior of Hydrophilic Micron Particles on a Planar Gas-Liquid Interface.

The behavior of hydrophilic micron particles impacting on the gas-liquid interface has been further experimentally studied using a high-speed camera at different surface tensions and dynamic viscosities of liquids. The results show that the impact behavior exhibits suspension and submergence modes, whose boundary cannot be clearly identified because the overlap between the impact velocity ranges occurs because of the unstable pinning of the three-phase contact line on the surface of hydrophilic particles. The liquid properties have little effect on the wettability of hydrophilic particles but greatly influence the hydrodynamic and capillary force exerted on the particles, leading to the expansion of the suspension mode range. In addition, the penetration probability changes little with the decrease in surface tension, while it significantly reduces with the increase in dynamic viscosity. A penetration probability model is predicted as an exponential function of the inertial and supporting forces, and the experimental values agree well with the predicted values. The outcomes of this research will be helpful for understanding the mechanism of particle-interface interaction and providing guidance for enhancing the separation of hydrophilic fine ash via a bubble scrubbing system.

Manipulating Clusters by Use of Competing N,O-Chelating Ligands: A Combined Crystallographic, Mass Spectrometric, and DFT Study.

Two heptanuclear discs, [Co7 (L1)6 (CH3 O)6 ]2+ (Co7 -L1) and [Co7 (L2)12 ](ClO4 )2 (Co7 -L2), have been prepared by the reaction of [Co(H2 O)6 ](ClO4 )2 with Schiff base HL1 (HL1=2-methoxy-6-[(methylimino)methyl]phenolate) or benzimidazole derivative HL2 (HL2=(1H-benzo[d]imidazol-2-yl)methanolate), respectively, at room temperature. In contrast, the reaction of [Co(H2 O)6 ](ClO4 )2 with a mixture of the two ligands, having similar coordination ability but different shapes, resulted in the butterfly-structured tetranuclear cobalt cluster [Co4 (L1)2 (L2)4 ](ClO4 )2 ⋅2H2 O (Co4 ). Electrospray ionization mass spectrometry (ESI-MS) analysis in real time revealed the {Co1 }→{Co2 }→{Co4 }→{Co7 } stepwise assembly when a single ligand was employed, but when both ligands were used only {Co4 } was obtained, an intermediate of the former reactions. Interestingly, ligand competition was evidenced in the assembly process of the reaction with two ligands; for example, [Co4 (L1)4 (L2)2 ]2+ and [Co4 (L1)3 (L2)3 ]2+ were the primary clusters observed at the beginning of the reaction, but [Co4 (L1)2 (L2)4 ]2+ was the final product. This observation suggests ligand exchange is taking place, and DFT calculations confirmed that these transformations were possible. By varying the ratio of ligands, the abundance of a certain species in the competitive reaction could be controlled.

Ligand Effect on the Single-Molecule Magnetism of Tetranuclear Co(II) Cubane.

A clear dependence on the ligand has been observed for the magnetic properties of a closely related series of Co(II) cubane structures, viz. [Co4(mbm or bm)4(ROH)4Br4] (1-MeOH, 1-EtOH, 2-MeOH, and 2-EtOH, where 1 = [Co4(mbm)4Br4], 2 = [Co4(bm)4Br4], bm = (1H-benzo[d]imidazol-2-yl)methanolate. and mbm = 1-Me-bm.) The [Co4(OR)4] cubane core consists of an octahedral CoII center chelated by the alkoxide oxygen and imidazole nitrogen atoms from monoanionic bm or mbm and coordinated by methanol/alcohol and bromine. Interestingly, electrospray ionization mass spectrometry (ESI-MS) indicates that 1-MeOH and 2-MeOH are unstable in methanol and transformed to the butterfly [Co4L6]2+ but that 1-EtOH and 2-EtOH are stable in ethanol. Their magnetic susceptibilities suggest ferromagnetic coupling between the nearest cobalt centers to give a theoretical S = 4 × 3/2 ground state with considerable magneto-crystalline behavior. The packing and intermolecular interactions appear to influence the geometry of the cubes and thus the anisotropy of cobalt, which leads to different blocking temperatures (TB). Consequently, the compounds with mbm, 1-MeOH and 1-EtOH, exhibit TB > 2 K as shown by the relaxation of magnetization in zero applied dc field where the barriers Ueff/kB are respectively 27 and 21 K and relaxation times are τ0 = 1.3 × 10-9 and 9.7 × 10-9 s. However, the compounds with bm, 2-MeOH and 2-EtOH, remain paramagnetic above 2 K and do not show nonlinear response of the ac susceptibilities. These findings reaffirm the subtle dependence of single-molecule magnetism on coordination geometry and intermolecular interaction.

Hierarchical Assembly and Aggregation-Induced Enhanced Emission of a Pair of Isostructural Zn14 Clusters.

Information of solid-state and solution structures is crucial in the characterization of molecular clusters and in advancing the understanding of their diverse properties. [Et3NH]2[Zn14(hmq)8(OH)4X10] [X = Cl and Br; H2hmq = 2-(hydroxymethyl)quinolin-8-ol] consist of a peanut-shaped Zn10O12 core, in which the Zn atoms occupy the faces and corners of an octahedron and are protected by bonded halogen atoms and bulky organic ligands. Observation of the [Zn14(hmq)8(OH)4X10]2- fragment in electrospray ionization mass spectrometry (ESI-MS) suggests that the cluster is stable in solution. ESI-MS analyses from dissolved crystals and mother liquors reveal that Zn(hmq) self-assembles to Zn5(hmq)4Cl, then dimerizes through four [OH]- bridges to Zn10(hmq)8(OH)4Cl2, and progressively captures four ZnCl2 one-by-one to [Zn14(hmq)8(OH)4Cl10]2-. Because the supramolecular interactions between the anion and cation in the solid suppress the rotation/vibration of the halogen atoms and confine the movable organic ligands on the rigid Zn-O core, both crystal phases exhibit intense photoluminescence, much stronger than that in solution. This is the first coordination cluster to exhibit "aggregation-induced enhanced emission". In addition, preliminary tests indicate that these coordination clusters are promising for organic-light-emitting-diode applications.

[Study on Biochar Properties Analysis with Scanning Electron Microscope-EnergyDispersive X-Ray Spectroscopy (SEM-EDX)].

Scanning Electron Microscope-Energy Dispersive X-ray spectroscopy (SEM-EDX) was applied to analyze the chemical and structural properties of biochars produced under different temperatures. Results showed that average carbon content (SEM C aver.) and maximum carbon content (SEM C max.) of miscanthus (MS) biochar increased as temperature increasing. There were significant and positive relationships between SEM C max., SEM C aver. and highest treatment temperature (HTT) (r were 0.76 and 0.86). SEM C max., SEM C aver. and dry combustion total carbon content had significant and positive relationships (r were 0.83 and 0.91), SEM C max. which was better than SEM C aver. So the carbon content of MS biochar which had good correlationship with temperature analyzed by SEM-EDX, SEM C max. could be used for composition analysis of biochar, scanning results could analyze structural properties of biochar effectively. This method is rapid, simple and stable. It also could analyze structure and composition of biochar simultaneously. It is a promising method that would be useful to study the structure and composition of biochar and other materials.

Safety profile of 0.0015% tafluprost eye drops in China: a post-marketing observational study.

AIM: To investigate the treatment pattern and safety of tafluprost for glaucoma and ocular hypertension (OH) in clinical practice in China. METHODS: This post-marketing observational study included patients who received tafluprost to lower intraocular pressure (IOP) within 30d between September 2017 and March 2020 in 20 hospitals in China. Adverse drug reactions (ADRs) during tafluprost treatment and within 30d after the treatment were collected. RESULTS: A total of 2544 patients were included in this study, of them 58.5% (1488/2544) had primary open angle glaucoma (POAG), 21.9% (556/2544) had OH and 19.7% (500/2544) used tafluprost for other reasons. Of 359 ADRs occurred in 10.1% (258/2544) patients, and no serious adverse event occurred. The most common ADR was conjunctival hyperemia (128 ADRs in 124 patients, 4.9%). Totally 1670 participants (65.6%) combined tafluprost with carbonic anhydrase inhibitors (CAIs; 37.1%, 620/1670), sympathomimetics (33.5%, 559/1670), ß-blockers (33.2%, 555/1670), other prostaglandin analogs (PGAs; 15.6%, 260/1670) and other eye drops (15.1%, 253/1670). The highest incidence of conjunctival hyperemia was noted in patients who received tafluprost in combination with other PGAs (23 ADRs in 23 patients, 8.8%, 23/260) and the lowest was in combination with CAIs (16 ADRs in 16 patients, 2.6%, 16/620). Tafluprost was applied in primary angle-closure glaucoma (41.6%, 208/500), after glaucoma surgery (17.8%, 89/500) and after non-glaucoma surgery (15.8%, 79/500). CONCLUSION: Tafluprost is safe for POAG and OH, and tolerable when combined with other eye drops and under various clinical circumstances.

[The analgesic effect of plexus anesthesia with buprenorphine in patients].

OBJECTIVE: To study the anesthetic effect of brachial plexus block by adding buprenorphine in local anesthetics and patients with intramuscular injection, and observe the anesthesia effects, the anesthesia maintenance time, postoperative analgesia effects. METHODS: 60 cases of upper limb to line, hand surgery patients from Sep. 2009 to Dec. 2009 in Tianjin Hospital were randomly divided into 3 groups. A (local anesthetics without buprenorphine, n = 20); B group (plus 2 µg/kg buprenorphine in local anesthetics, n = 20); C group (intramuscular injection 2 µg/kg buprenorphine before anesthesia, n = 20). With B/BRAMN-STIMMPLEX-DIG nerve stimulator guided positioning of brachial plexus block of axillary line. 3 groups of patients recorded (1) the onset time of narcotic; (2) the duration of anesthesia; (3) the surgery time; (4) the pain score of postoperative 4, 8, 12, 24, 36, 48, 72 h; (5) the incidence of nausea and vomiting; (6) observed other side effects. RESULTS: the patients' age, weight, sex, operation time of the 3 groups had no significant difference between the comparison (P > 0.05); anesthesia onset time between the 3 groups showed no significant difference (P > 0.05). Duration of anesthesia: A, C group was significantly shorter than the B group (P < 0.01); pain score at 4 h, A and B, C no significant difference between groups (P > 0.05); 8, 12, 24 h, when group A was significantly higher than the B, C group (P < 0.01), 36, 48, 72 h when the A group and B, C no significant difference between groups (P > 0.05); the incidence of nausea A group 10%, B group 20%, C group 20%. Vomiting, the incidence of A, B group was 0, C group 30%. CONCLUSION: Buprenorphine adding local anesthetics in brachial plexus block or intramuscular injection buprenorphine before a block can be to achieve a satisfactory effect of postoperative analgesia, buprenorphine adding local anesthetics in brachial plexus block Narcotic maintenance of anesthesia time can be extended and have a significant effect and fewer adverse reactions.

Highly dispersed and stabilized Co3O4/C anchored on porous biochar for bisphenol A degradation by sulfate radical advanced oxidation process.

Cobalt oxide and porous carbon materials are desirable catalysts for peroxymonosulfate (PMS) advanced oxidation reaction in the degradation of organic pollutants. Herein, carbon-coated Co3O4 (Co3O4/C) mounted biochar (BC) composites (Co3O4/C-BC) with a three-dimensional spongy-like network were constructed by driving a dual-precursors of zeolitic imidazolate framework (ZIF-67) and Eichhornia crassipes. Considering the unique structures and compositions, the Co3O4/C-BC composites enhanced the dispersion of Co3O4/C nanoparticles, minimized the Co leaching, mediated the size of Co3O4/C nanoparticles, and enhanced the overall catalytic activity. Co3O4/C-BC effectively activated PMS for bisphenol A (BPA) degradation along with satisfactory reusability in advanced oxidation reaction. Electron paramagnetic resonance and radical quenching tests revealed that the generation and effect of sulfate and hydroxyl radicals in reaction process. This work not only provided a promising catalyst for the degradation of organic pollutants but also expanded BPA degradation pathway and PMS activation mechanism.

PLG inhibits Hippo signaling pathway through SRC in the hepatitis B virus-induced hepatocellular-carcinoma progression.

PURPOSE: Hepatitis B virus (HBV) infection is one main cause of hepatocellular carcinoma (HCC), but the mechanisms of pathogenesis still remain unclear. METHODS: We screened the 1351 differentially expressed genes related to HBV-induced HCC by bioinformatics analysis from databases and found that Plasminogen (PLG) may be a key gene in HBV-induced HCC progression. Then, we used a series of experiments in vivo and in vitro to explore the roles of PLG in HBV-HCC progression, such as qRT-PCR, western blot, ELISA, flow cytometry and TUNEL assay, subcutaneous xenografts and histopathological analysis to reveal the underlying mechanisms. RESULTS: PLG was over-expressed in HBV positive hepatocellular carcinoma tissues and cells. PLG silencing promoted HBV-HCC cell apoptosis in vitro and suppressed the growth of HBV-induced HCC xenografts in vivo both through inhibiting HBV replication. Then, GO and KEGG analysis of these differentially expressed genes revealed that the Hippo pathway was the key pathway involved in HBV-induced HCC, and SRC, a downstream target gene of PLG, was highly expressed in HBV-induced HCC and related to the Hippo pathway. Thus, we speculated that PLG promoted HBV-induced HCC progression through up-regulating and activating the expression of SRC and promoting Hippo signaling pathway function on HBV-HCC cell survival. CONCLUSION: Our study suggests PLG may be an activator of HBV-infected hepatocellular carcinoma development, as a novel prognostic biomarker and therapeutic target for HBV-HCC.

Multifunctional Textiles/Metal-Organic Frameworks Composites for Efficient Ultraviolet Radiation Blocking and Noise Reduction.

Ultraviolet radiation (UVR) and noise are the ubiquitous environmental hazards with considerable detrimental effects on the physiological and psychological health of humans. Exploiting efficient protective materials that can be extensively used in daily life for simultaneous anti-UVR and noise mitigation will be of crucial importance, but it is still a significant challenge in materials design. Herein, we developed a series of protective textiles for efficient anti-UVR and noise reduction via MOFs nanocrystal-modified cotton textiles. The formation of MOFs@cotton textiles was confirmed by using electron microscopy, X-ray diffraction, infrared spectroscopy, and X-ray photoelectron spectroscopy. The fabricated MOFs@cotton textiles exhibited substantial improvement in the UVR blocking and acoustic absorption properties compared to blank cotton textiles. Therefore, this work provides a good strategy for designing and preparing multifunctional protective textiles.

[Trabeculotomy on primary congenital glaucoma: a retrospective study of 164 cases (257 eyes)].

OBJECTIVE: To study the relationship of long-term effect of trabeculotomy on primary congenital glaucoma and the related risk factors. METHOD: 164 consecutive patients with primary congenital glaucoma (257 eyes), underwent initial surgery of trabeculotomy between 1996 and 2007. Follow-up was conducted for 30.9 (8.6 - 58.3) months, with a follow-up rate of 89.02%. Multivariate analysis by Logistic regression was conducted to analyze the relation of the factors including age of onset, time between onset and operation, preoperative intraocular pressure, clarity of cornea, and corneal diameter to the failure of surgery. Cox proportional hazards regression modeling was used to analyze the factors related to success of surgery. RESULT: Multivariate logistic regression showed that the preoperative intraocular pressure (IOP) and clarity of cornea were independent risk factors for final outcome (OR(IOP) = 1.408, P = 0.047, and OR(CLA) = 1.691, P = 0.019). Cox regression showed that clarity of cornea was the factor related to the surgery success time (OR(CLA) = 1.632, P = 0.008). CONCLUSION: Clarity of cornea reflexes the condition of primary congenital glaucoma more stably than IOP. It is possible to prognosticate the surgical outcome to combine the clarity of cornea with the IOP value before operation.

Nanoscale Zero-Valent Iron and Chitosan Functionalized Eichhornia crassipes Biochar for Efficient Hexavalent Chromium Removal.

Sorption is widely used for the removal of toxic heavy metals such as hexavalent chromium (Cr(VI)) from aqueous solutions. Green sorbents prepared from biomass are attractive, because they leverage the value of waste biomass and reduce the overall cost of water treatment. In this study, we fabricated biochar (BC) adsorbent from the biomass of water hyacinth (Eichhornia crassipes), an invasive species in many river channels. Pristine BC was further modified with nanoscale zero-valent iron (nZVI) and stabilized with chitosan (C) to form C-nZVI-BC. C-nZVI-BC adsorbent showed high hexavalent chromium sorption capacity (82.2 mg/g) at pH 2 and removed 97.34% of 50 mg/L Cr(VI) from aqueous solutions. The sorption capacity of chitosan-nZVI-modified biochar decreased while increasing the solution pH value and ionic strength. The results of a sorption test indicated that multiple mechanisms accounted for Cr(VI) removal by C-nZVI-BC, including complexation, precipitation, electrostatic interactions, and reduction. Our study suggests a way of adding value to biomass waste by considering environmental treatment purposes.

Monitoring fragmentation and oligomerization of a di-µ-methoxo bridged copper(ii) complex: structure, mass spectrometry, magnetism and DFT studies.

Analyses of the structural information of molecular fragments from the mass spectra of the solid-state products and their reaction solutions allow for the understanding of their formation and of their diverse properties. The reaction of CuCl2 and (1-methyl-1H-benzo[d]imidazole-2-yl)methanol (HL) led only to crystals containing molecular dimers of [Cu2(L)2Cl2] (Cu2). The CuII-CuII distance and Cu-OR-Cu angle in the structure are 3.044 Å and 104.8°, respectively. The magnetic susceptibility (3-400 K) is characterized by a very strong intradimer antiferromagnetic interaction of J = -465 and interdimer zj = -0.83 cm-1. But mass spectrometry of a dissolved single crystal in different source energies identifies both its fragmentation and oligomerization to [Cu] and [Cu]. DFT calculations give the relative stabilization energies of the fragments observed in ESI-MS to provide a formation process.

[Comparison on fungal molecular ecological networks of agricultural soils with different latitudes in the black soil region of Northeast China]. / 东北黑土区不同纬度农田土壤真菌分子生态网络比较.

To investigate the differences of fungal network structures and interaction among fungal species of in black soil region of Northeast China, Illumina MiSeq sequencing was used to reveal the fungal communities in the three long-term fertilization experimental fields. Fungal molecular ecological networks were constructed based on random matrix theory (RMT). The results demonstrated that Ascomycota, Basidiomycota and Zygomycota were the dominant phyla and Hypocreales, Pleosporales and Sordariales were the dominant order, but the relative abundance of some dominant taxa significantly varied in different locations. Fungal molecular ecological network structures in three locations showed significant difference, with more complex fungal network being observed in north location with more competitive relations among species. The fungal network in south location was more easily disturbed by environmental perturbations with less stability. Only seven shared nodes were detected among three fungal molecular ecological networks. There were large differences in connectivity of shared nodes within individual fungal network. The subnetwork of Hypocreales was gradual complex from south to north location while subnetwork of Pleosporales presented reversed trend. The key species of south, middle and north locations were Chaetomiaceae, Pleosporales and Penicillium coralligerum, respectively. Soil pH and total N content were the main soil properties simultaneously influencing three fungal networks.

Sensitized near infrared emission through supramolecular d → f energy transfer within an ionic Ru(ii)-Er(iii) pair.

The newly synthesized ionic triple salt Ru-Er, {[RuII(bpy)2(dbim)][ErIII(hfac)4][CF3COO]·H2O} (bpy = 2,2'-bipyridine; hfac- = hexafluoroacetylacetonate; dbim = 2,2'-dibenzimidazole) exhibits near-infrared (NIR) emission at 1535 nm by intermolecular Ru → Er (d → f) energy transfer across supramolecular interactions when pumped within the Ru(ii) 3MLCT band. It is the first such observation for a transition metal-lanthanide ionic pair.

Positive PCNA and Ki-67 Expression in the Testis Correlates with Spermatogenesis Dysfunction in Fluoride-Treated Rats.

The present study aimed to evaluate the effect of fluoride (F) on spermatogenesis in male rats. F- at 50 and 100 mg/L was administered for 70 days, after which the testicular and epididymis tissues were collected to observe the histopathological structure under a light microscope. The ultrastructure of the testis and sperm was also examined via transmission electron microscopy. The apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki-67, in the testicular and epididymis tissues, were assayed through immunohistochemistry. F- at 50 and 100 mg/L significantly damaged the structure of the testis and epididymis, and the testis and sperm ultrastructure exhibited various changes, including mitochondrial swelling and vacuolization, and apsilated and raised sperm membrane. F treatment significantly increased spermatogenic cell apoptosis in the testis. PCNA (P < 0.01) and Ki-67 (P < 0.01) also presented positive expression in the testis. By comparison, no significant changes occurred in the epididymis. In summary, excessive F intake results in spermatogenesis dysfunction by damaging the testicular structure and inducing spermatogenic cell apoptosis in male rats. The positive expression level of PCNA and Ki-67 was a good response to spermatogenesis dysfunction.

Regulating structural dimensionality and emission colors by organic conjugation between SmIII at a fixed distance.

The conjugation of bridging bis(diphenylphosphine oxide) alkane or arene ligands was found to control the structural dimensionality and the emission color of complexes from reactions with SmIII(hfac)3(H2O)2 (hfac- = hexafluoroacetylacetonato) while retaining the SmSm distances. Bis(diphenylphosphine oxide)-1,4-butane (L1) affords a one-dimensional (1D) ribbon {Sm(hfac)3(L1)}∞ (1) that emits red color, while bis(diphenyl-phosphinoyl)-1,4-benzene (L2) results in a two-dimensional (2D) network {Sm(hfac)2(CF3COO)(L2)3}∞ (2) and near-white emission, but bis(diphenyl-phosphinoyl)-9,10-anthracene (L3) forms a zero-dimensional (0D) cyclic structure {Sm(hfac)3(L3)}2 (3) with strong ππ interactions that emit green color. Noticeably, the conjugation change is accompanied by a configurational change of coordination from trans for 1 and 2 to cis for 3. The color change is associated with the superposition of ligand and Sm based electronic band energies and their intensities. Such white light emission by a single compound having contributions from different building components is quite rare.

Ferromagnetic coupling in copper benzimidazole chloride: structural, mass spectrometry, magnetism, and DFT studies.

Herein, quasi-square planar CuII(Hmbm)Cl2 (CBC, Hmbm = (1-methyl-1H-benzo[d]imidazol-2-yl)methanol) was arranged in a pseudo orthogonal way to form Cl-bridged chains, and further ππ interactions resulted in distorted hexagonal layers. DFT calculations reveal a bond strength order of Cu-Cl > Cu-O/N ≫ CuCl. ESI-MS data reveal several small fragments from CBC, but oligomeric [Cu], [Cu], and [Cu] for non-zero in-source energies; MS data indicates the occurrence of several chemical processes, viz. splitting of the ligand, oligomerization, and redox reaction of alcohol to aldehyde and CuII to CuI. Gibbs free energies for the fragments were estimated using DFT. The magnetic susceptibility was modeled with the ferromagnetic coupling J(Cu-Cl2aCu) = +0.99(30) cm-1 and J'(ππ) = +0.35(16) cm-1 and g = 2.38(2). HF-EPR determined the anisotropic g-values, gx = 2.24, gy = 2.16, and gz = 2.09, and a hyperfine constant of Az = 450 G. DFT calculations from crystal structure data reveal a J(Cu-Cl2aCu) of +3.6 at 296 K and +4.1 cm-1 at 90 K that dominates the magnetic properties, whereas J'(ππ) = 0.04 cm-1 is negligibly small.

Evaluating the association between pathological myopia and SNPs in RASGRF1. ACTC1 and GJD2 genes at chromosome 15q14 and 15q25 in a Chinese population.

BACKGROUND: This study investigated the association of the 27 SNPs located in RASGRF1. GJD2, and ACTC1 genes with pathological myopia in a Chinese Han population. METHODS: Myopia patients were stratified according to whether they did (n = 274) or did not (n = 131) have myopic macular degeneration (MMD). The SNPbrowser software was used to identify specific SNPs for analysis and minimal allele frequency of >20%, and a pairwise r(2) < 0.85 were genotyped using MALDI-TOF mass spectrometry. RESULTS: Before controlling for false discovery rate, the frequency of the rs1867315 C/C genotype compared with healthy controls was lower in the myopia group (p = 0.006) and in myopia patients without macular degeneration (p = 0.019). The frequency of the rs670957A/A genotype was also lower in patients without MMD compared with controls (p = 0.045). For rs2070664, the frequency of the A allele was higher in the patients with MMD compared to those without MMD (p = 0.032). After controlling for a false discovery rate of 5%, there was no significant difference in genotype and allele frequencies between these groups. CONCLUSION: In this study, there was no association of the analyzed SNPs located in RASGRF1. GJD2, and ACTC1 with pathological myopia, suggesting that SNPs included in our study have no or a limited role in causing pathologic myopia in this Chinese Han population.